LOCUS       sequence-213713-        7080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 351-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 351-972
FEATURES             Location/Qualifiers
     source          1..7080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2463..2483)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2463..2481)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2502..2518)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2502..2518)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2515..2537)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2526..2542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2550..2556,2557..2574,2575..2580))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2595..2616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2733..2750)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2904..3492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2984..3003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3663..4454,4455..4523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4286..4305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4524..4628)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4665..5168
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5209..5227
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5265..5287)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5387..5406
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5427..5707
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5708..6382
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6485..6940)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6622..6643)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6834..6853
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7066..7080,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7080,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgca tcttactggg ctgatgagtt gtatcaaaga
      361 ctgcctaatg gatgatcacg ttccggtgcg aattgttaca gcacatactt tatcaacact
      421 agcagaaaat tcttatccat atggtatcga agttttcaac gttgtactgg agccattatg
      481 gaagggtata agaagtcatc gtggtaaagt gctatcttct tttttaaaag ctgttggatc
      541 tatgatccca ttgatggacc cagagtatgc cggttattat accacagaag caatgagaat
      601 aattagacgt gaattcgact ctcccgatga tgaaatgaaa aagaccattc ttttagtatt
      661 acaaaaatgt agcgctgtag aatccataac gccaaaattt ctgagagaag aaatcgcgcc
      721 agaattcttt caaaaatttt gggtcagacg agtggcattg gatagaccat taaacaaagt
      781 tgttacatat actactgtta cattagcaaa gaaattaggt tgttcttata ccattgataa
      841 attattaaca ccattgagag atgaagctga gccgttcaga acaatggctg tccatgctgt
      901 tactagaaca gtaaacttat tagggacagc agatttagat gagagattag aaacgaggct
      961 tattgatgcg ctcttgatag catttcaaga acaaacaaat agtgattcta taatctttaa
     1021 agggtttggg gcagtgacgg tgtcacttga catccgtatg aaaccgtttt tagcacccat
     1081 tgtgagtacg attttaaatc atttaaagca taaaactccc ttggttcgtc aacatgcagc
     1141 agatctatgt gccatactaa taccagtcat caagaactgt cacgaattcg aaatgttgaa
     1201 taaattgaac attatattgt atgaatcctt aggcgaagta tatccagagg tattaggttc
     1261 tataatcaac gctatgtact gtattactag cgtaatggat ttggataaac tacaacctcc
     1321 tattaatcaa attttaccaa ccttaactcc aattttaaga aacaagcata gaaaagtcga
     1381 agtaaacact atcaaatttg ttggacttat tggtaaatta gcacctacct atgcacctcc
     1441 taaagaatgg atgagaatat gttttgaact attagaactt ttgaaaagta caaataaaga
     1501 aataagaaga tcagcaaatg ccacgtttgg attcattgct gaagcaattg gacctcacga
     1561 tgttcttgtt gcgttattga acaatttaaa ggttcaagaa cgtcagttac gtgtatgcac
     1621 ggctgttgct atcggtatcg ttgctaaagt gtgtggtcct tacaacgtgc tgccagtaat
     1681 catgaatgaa tacacaacac cagagactaa tgttcaaaac ggtgttctca aggccatgtc
     1741 attcatgttt gagtacattg gtaacatgtc taaggattac atatacttca taacaccatt
     1801 attagaagat gcactcaccg atagggattt agttcatcgt cagacagcgt cgaacgtaat
     1861 aacccactta gctttaaact gttcaggtac aggtcacgaa gatgccttca ttcatttaat
     1921 gaatcttcta atacccaata tttttgaaac ttcaccacat gctattatgc gtattttaga
     1981 aggtttggag gcactgagtc aagctcttgg ccctggacta ttcatgaact acatatgggc
     2041 aggattgttc catccagcga agaatgtaag gaaagcattt tggagggtgt ataataacat
     2101 gtacgtaatg taccaggacg ccatggtacc tttttacccc gttacaccag acaacaatga
     2161 agaatatata gaagaactgg atttagttct gtgaatatat atagagcata taaataacca
     2221 caacttgtaa gaatatttta cttgacacac ctatatcatc acgtcacgat gccttcatag
     2281 attatgatga aatataaaca gaacctgcta ttggaaaaaa aagggcagga aaaaattttg
     2341 tgaaagtgag taaaagaaag atgaagagcg agacatggat aataagaaat agatgtcact
     2401 aatggacaat tggaagactg atatggaaag ttacgatgaa ggaggagctc cagcttttgt
     2461 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2521 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2581 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2641 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2701 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2761 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2821 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2881 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2941 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3001 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3061 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3121 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3181 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3241 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3301 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3361 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3421 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3481 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3541 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3601 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3661 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3721 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3781 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3841 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3901 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3961 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4021 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4081 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4141 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4201 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4261 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4321 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4381 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4441 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4501 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4561 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4621 gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa
     4681 ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa
     4741 gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc
     4801 caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt
     4861 catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg
     4921 ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt
     4981 acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc
     5041 ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac
     5101 agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc
     5161 aagcgatccg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5401 attgtactga gagtgcacca tagatcacga cattactata tatataatat aggaagcatt
     5461 taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga tggcagtagt
     5521 ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg atccggagct
     5581 tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga
     5641 gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc
     5701 ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg
     5761 cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat
     5821 tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag
     5881 tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg
     5941 taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca
     6001 actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa
     6061 aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca
     6121 ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa
     6181 ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc
     6241 tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt
     6301 tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt
     6361 cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg
     6421 cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     6481 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     6541 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     6601 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     6661 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     6721 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     6781 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     6841 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     6901 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     6961 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     7021 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
//
