LOCUS       sequence-213715-        6717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 472-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 472-972
FEATURES             Location/Qualifiers
     source          1..6717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2100..2120)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2100..2118)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2139..2155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2139..2155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2152..2174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2163..2179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2187..2193,2194..2211,2212..2217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2232..2253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2370..2387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2541..3129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2621..2640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3300..4091,4092..4160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3923..3942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4161..4265)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4302..4805
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     4846..4864
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4902..4924)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5024..5043
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5064..5344
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5345..6019
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6122..6577)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6259..6280)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6471..6490
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(6703..6717,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6717,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgcc aaaatttctg agagaagaaa tcgcgccaga
      361 attctttcaa aaattttggg tcagacgagt ggcattggat agaccattaa acaaagttgt
      421 tacatatact actgttacat tagcaaagaa attaggttgt tcttatacca ttgataaatt
      481 attaacacca ttgagagatg aagctgagcc gttcagaaca atggctgtcc atgctgttac
      541 tagaacagta aacttattag ggacagcaga tttagatgag agattagaaa cgaggcttat
      601 tgatgcgctc ttgatagcat ttcaagaaca aacaaatagt gattctataa tctttaaagg
      661 gtttggggca gtgacggtgt cacttgacat ccgtatgaaa ccgtttttag cacccattgt
      721 gagtacgatt ttaaatcatt taaagcataa aactcccttg gttcgtcaac atgcagcaga
      781 tctatgtgcc atactaatac cagtcatcaa gaactgtcac gaattcgaaa tgttgaataa
      841 attgaacatt atattgtatg aatccttagg cgaagtatat ccagaggtat taggttctat
      901 aatcaacgct atgtactgta ttactagcgt aatggatttg gataaactac aacctcctat
      961 taatcaaatt ttaccaacct taactccaat tttaagaaac aagcatagaa aagtcgaagt
     1021 aaacactatc aaatttgttg gacttattgg taaattagca cctacctatg cacctcctaa
     1081 agaatggatg agaatatgtt ttgaactatt agaacttttg aaaagtacaa ataaagaaat
     1141 aagaagatca gcaaatgcca cgtttggatt cattgctgaa gcaattggac ctcacgatgt
     1201 tcttgttgcg ttattgaaca atttaaaggt tcaagaacgt cagttacgtg tatgcacggc
     1261 tgttgctatc ggtatcgttg ctaaagtgtg tggtccttac aacgtgctgc cagtaatcat
     1321 gaatgaatac acaacaccag agactaatgt tcaaaacggt gttctcaagg ccatgtcatt
     1381 catgtttgag tacattggta acatgtctaa ggattacata tacttcataa caccattatt
     1441 agaagatgca ctcaccgata gggatttagt tcatcgtcag acagcgtcga acgtaataac
     1501 ccacttagct ttaaactgtt caggtacagg tcacgaagat gccttcattc atttaatgaa
     1561 tcttctaata cccaatattt ttgaaacttc accacatgct attatgcgta ttttagaagg
     1621 tttggaggca ctgagtcaag ctcttggccc tggactattc atgaactaca tatgggcagg
     1681 attgttccat ccagcgaaga atgtaaggaa agcattttgg agggtgtata ataacatgta
     1741 cgtaatgtac caggacgcca tggtaccttt ttaccccgtt acaccagaca acaatgaaga
     1801 atatatagaa gaactggatt tagttctgtg aatatatata gagcatataa ataaccacaa
     1861 cttgtaagaa tattttactt gacacaccta tatcatcacg tcacgatgcc ttcatagatt
     1921 atgatgaaat ataaacagaa cctgctattg gaaaaaaaag ggcaggaaaa aattttgtga
     1981 aagtgagtaa aagaaagatg aagagcgaga catggataat aagaaataga tgtcactaat
     2041 ggacaattgg aagactgata tggaaagtta cgatgaagga ggagctccag cttttgttcc
     2101 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     2161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4261 ccgcgcacat ttccccgaaa agtgccacct gggtcctttt catcacgtgc tataaaaata
     4321 attataattt aaatttttta atataaatat ataaattaaa aatagaaagt aaaaaaagaa
     4381 attaaagaaa aaatagtttt tgttttccga agatgtaaaa gactctaggg ggatcgccaa
     4441 caaatactac cttttatctt gctcttcctg ctctcaggta ttaatgccga attgtttcat
     4501 cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt ttacatttta cttatcgtta
     4561 atcgaatgta tatctattta atctgctttt cttgtctaat aaatatatat gtaaagtacg
     4621 ctttttgttg aaatttttta aacctttgtt tatttttttt tcttcattcc gtaactcttc
     4681 taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa taaacacaga
     4741 gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag
     4801 cgatccgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4861 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4921 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4981 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     5041 gtactgagag tgcaccatag atcacgacat tactatatat ataatatagg aagcatttaa
     5101 tagaacagca tcgtaatata tgtgtacttt gcagttatga cgccagatgg cagtagtgga
     5161 agatattctt tattgaaaaa tagcttgtca ccttacgtac aatcttgatc cggagctttt
     5221 ctttttttgc cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg
     5281 agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg
     5341 gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag tttgcggctt
     5401 gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc tgggtattat
     5461 atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa tttcaagtct
     5521 tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa
     5581 tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata tcgtccaact
     5641 gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag ttattaaaag
     5701 actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga aacctcattc
     5761 gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg attggaactc
     5821 gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta tgttagctgg
     5881 tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta ttggtgttga
     5941 tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag caaatttcgt
     6001 caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt gtttgtgcac
     6061 ttgccgatct atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6121 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     6181 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     6241 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     6301 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     6361 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     6421 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     6481 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     6541 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca
     6601 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6661 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgtt
//
