LOCUS       sequence-213716-        9551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gamma-Retroviral transfer vector for expressing glutminase isoform 
            KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Sandra Martha Gomes Dias
  TITLE     HuR (ELAVL1) silencing sensitizes triple-negative breast cancer to 
            glutaminase-targeted therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQC hKGA.wt V5-His IRES G418
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             586..761
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    824..1023
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1224..1640
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1570..1592
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1608..1624
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1677..1980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1981..2184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2181..2205
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4426..4467
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4477..4494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4539..5105
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4699..4716)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4926..4945
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5119..5913
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5173..5192)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5783..5802
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6330..6755
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6858..6880)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7016..7036)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7021..7350
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7201..7336
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7372..7391
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7472..7489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7643..8231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7723..7742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8402..9193,9194..9262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9025..9044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9263..9367)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9435..9453
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca
      601 caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc
      661 ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc
      721 tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt
      781 tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt
      841 atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact
      901 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac
      961 ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct
     1021 gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg
     1081 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc
     1141 gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1201 ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag
     1261 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1321 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1381 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1441 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1501 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1561 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1621 tcactccttc tctaggcgcc ggaattgaag atctggggga tcgatcctct agagtccgtt
     1681 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1741 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1801 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     1861 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1921 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1981 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2041 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2101 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2161 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2221 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
     2281 cggtgcattg gaacggacct gcaggaattg atccgcggcc gcaccgggga tccagtaccc
     2341 ttcaccatga tgcggctgcg aggctcgggg atgctgcggg acctgctcct gcggtcgccc
     2401 gccggcgtga gcgcgactct gcggcgggca cagcccttgg tcaccctgtg ccggcgtccc
     2461 cgaggcgggg gacggccggc cgcgggcccg gctgccgccg cgcgactcca cccgtggtgg
     2521 ggcgggggcg gctggccggc ggagcccctc gcgcggggcc tgtccagctc tccttcggag
     2581 atcttgcagg agctgggcaa ggggagcacg catccgcagc ccggggtgtc gccacccgct
     2641 gccccggcgg cgcccggccc caaggacggc cccggggaga cggacgcgtt tggcaacagc
     2701 gagggcaaag agctggtggc ctcaggtgaa aataaaataa aacagggtct gttacctagc
     2761 ttggaagatt tgctgttcta tacaattgct gaaggacaag agaaaatacc tgttcataaa
     2821 tttattacag cactcaaatc tacaggattg cgaacgtctg atcccaggtt gaaagagtgt
     2881 atggatatgt taagattaac tcttcaaaca acatcagatg gtgtcatgct agacaaagat
     2941 ctttttaaaa aatgtgttca gagcaacatt gttttgttga cacaagcatt tagaagaaag
     3001 tttgtgattc ctgactttat gtcttttacc tcacacattg atgagttata tgaaagtgct
     3061 aaaaagcagt ctggaggaaa ggttgcagat tatattcctc aactggccaa attcagtccc
     3121 gatttgtggg gtgtgtctgt ttgtacagta gatggacaga ggcattctac tggagatacc
     3181 aaagttccct tctgtcttca gtcctgtgta aaacctttga aatatgccat tgctgttaat
     3241 gatcttggaa ctgaatatgt gcatcgatat gttggaaaag agccgagtgg actaagattc
     3301 aacaaactat ttttgaatga agatgataaa ccacataatc ctatggtaaa tgctggagca
     3361 attgttgtga cttcactaat aaagcaagga gtaaataatg ctgaaaaatt tgactatgtc
     3421 atgcagtttt tgaataagat ggctggtaat gaatatgttg gattcagtaa tgcaacgttt
     3481 cagtctgaaa gagaaagtgg agatcgaaat tttgcaatag gatattactt aaaagaaaag
     3541 aagtgttttc cagaaggcac agacatggtt ggtatattag acttctactt ccagctgtgc
     3601 tccattgaag tgacttgtga atcagccagt gtgatggctg cgacactggc taatggtggt
     3661 ttctgcccaa ttactggtga aagagtactg agccctgaag cagttcgaaa tacattgagt
     3721 ttgatgcatt cctgtggcat gtatgacttc tcagggcagt ttgctttcca tgttggtctt
     3781 cctgcaaaat ctggagttgc tgggggcatt cttttagttg tccccaatgt tatgggtatg
     3841 atgtgctggt ctcctcctct ggataagatg ggcaacagtg ttaagggaat tcacttttgt
     3901 cacgatcttg tttctctgtg taatttccat aactatgata atttgagaca ctttgcaaaa
     3961 aaacttgatc ctcgaagaga aggtggtgat caaagggtaa agtcagtgat aaatcttttg
     4021 tttgctgcat atactggaga tgtgtctgca cttcgaagat ttgctttgtc agctatggac
     4081 atggaacagc gggactatga ttctagaaca gcactccatg tagctgctgc agagggtcat
     4141 gttgaagttg ttaaattttt gctggaagcc tgcaaagtaa accctttccc caaggacagg
     4201 tggaataaca ctcccatgga tgaagcactg cactttggac accatgatgt atttaaaatt
     4261 ctccaagaat accaagtcca gtacacacct caaggagatt ctgacaacgg gaaggaaaat
     4321 caaaccgtcc ataagaatct tgatggattg ttgaagggtc aagacaattc tgcagatatc
     4381 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     4441 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     4501 taaactccaa agcatcgatg gtagcggccg cggtgaattc cgcccccccc cctaacgtta
     4561 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4621 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     4681 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     4741 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     4801 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4861 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4921 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4981 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     5041 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     5101 gataagcttg ccacaaccat ggctgaacaa gatggattgc acgcaggttc tccggccgct
     5161 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5221 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5281 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5341 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5401 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5461 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5521 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5581 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5641 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5701 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5761 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5821 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5881 gccttctatc gccttcttga cgagttcttc tgagcgggat cggctagagt cgacccggac
     5941 gggacgctcc tgcgcctgat acagaacgaa ttgcttgcag gcatctcatg agtgtgtctt
     6001 cccgttttcc gcctgaggtc actgcgtgga tggagcgctg gcgcctgctg cgcgacggcg
     6061 agctgctcac cacccactcg agggctgcag cgctgcagag gccgagtgca gaactgctcc
     6121 aaagggacct caaggctttc cgagggacac taggctgact ccatcgagcc agtgtagaga
     6181 taagcttatc gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt
     6241 ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag
     6301 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     6361 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     6421 ttcagatcaa ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt
     6481 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     6541 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     6601 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     6661 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     6721 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     6781 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc
     6841 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     6901 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     6961 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat
     7021 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7081 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7141 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7201 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7261 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7321 ggcttttttg gaggcctagg cttttgcaaa aagcttactg gcttaactat gcggcatcag
     7381 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     7441 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8041 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8701 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9001 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9241 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9301 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9361 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9421 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttagcttggc
     9481 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     9541 taccgccatg t
//
