LOCUS       sequence-213717-        9562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gamma-Retroviral transfer vector for expressing glutminase isoform 
            KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Sandra Martha Gomes Dias
  TITLE     HuR (ELAVL1) silencing sensitizes triple-negative breast cancer to 
            glutaminase-targeted therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQC hKGA.S286A V5-His IRES G418
FEATURES             Location/Qualifiers
     source          1..9562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4353..4394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4404..4421
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(4470..5044,5045..5047,5048..5056)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4637..4654)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4864..4883
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5057..5851
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5111..5130)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5721..5740
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6268..6693
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6796..6818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6954..6974)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6959..7288
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7139..7274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7310..7329
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7410..7427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7581..8169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7661..7680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8340..9131,9132..9200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8963..8982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9201..9305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9373..9391
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9491..9562,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggggatcc agtacccttc accatgatgc
     2281 ggctgcgagg ctcggggatg ctgcgggacc tgctcctgcg gtcgcccgcc ggcgtgagcg
     2341 cgactctgcg gcgggcacag cccttggtca ccctgtgccg gcgtccccga ggcgggggac
     2401 ggccggccgc gggcccggct gccgccgcgc gactccaccc gtggtggggc gggggcggct
     2461 ggccggcgga gcccctcgcg cggggcctgt ccagctctcc ttcggagatc ttgcaggagc
     2521 tgggcaaggg gagcacgcat ccgcagcccg gggtgtcgcc acccgctgcc ccggcggcgc
     2581 ccggccccaa ggacggcccc ggggagacgg acgcgtttgg caacagcgag ggcaaagagc
     2641 tggtggcctc aggtgaaaat aaaataaaac agggtctgtt acctagcttg gaagatttgc
     2701 tgttctatac aattgctgaa ggacaagaga aaatacctgt tcataaattt attacagcac
     2761 tcaaatctac aggattgcga acgtctgatc ccaggttgaa agagtgtatg gatatgttaa
     2821 gattaactct tcaaacaaca tcagatggtg tcatgctaga caaagatctt tttaaaaaat
     2881 gtgttcagag caacattgtt ttgttgacac aagcatttag aagaaagttt gtgattcctg
     2941 actttatgtc ttttacctca cacattgatg agttatatga aagtgctaaa aagcagtctg
     3001 gaggaaaggt tgcagattat attcctcaac tggccaaatt cagtcccgat ttgtggggtg
     3061 tgtctgtttg tacagtagat ggacagaggc attctactgg agataccaaa gttcccttct
     3121 gtcttcaggc ctgtgtaaaa cctttgaaat atgccattgc tgttaatgat cttggaactg
     3181 aatatgtgca tcgatatgtt ggaaaagagc cgagtggact aagattcaac aaactatttt
     3241 tgaatgaaga tgataaacca cataatccta tggtaaatgc tggagcaatt gttgtgactt
     3301 cactaataaa gcaaggagta aataatgctg aaaaatttga ctatgtcatg cagtttttga
     3361 ataagatggc tggtaatgaa tatgttggat tcagtaatgc aacgtttcag tctgaaagag
     3421 aaagtggaga tcgaaatttt gcaataggat attacttaaa agaaaagaag tgttttccag
     3481 aaggcacaga catggttggt atattagact tctacttcca gctgtgctcc attgaagtga
     3541 cttgtgaatc agccagtgtg atggctgcga cactggctaa tggtggtttc tgcccaatta
     3601 ctggtgaaag agtactgagc cctgaagcag ttcgaaatac attgagtttg atgcattcct
     3661 gtggcatgta tgacttctca gggcagtttg ctttccatgt tggtcttcct gcaaaatctg
     3721 gagttgctgg gggcattctt ttagttgtcc ccaatgttat gggtatgatg tgctggtctc
     3781 ctcctctgga taagatgggc aacagtgtta agggaattca cttttgtcac gatcttgttt
     3841 ctctgtgtaa tttccataac tatgataatt tgagacactt tgcaaaaaaa cttgatcctc
     3901 gaagagaagg tggtgatcaa agggtaaagt cagtgataaa tcttttgttt gctgcatata
     3961 ctggagatgt gtctgcactt cgaagatttg ctttgtcagc tatggacatg gaacagcggg
     4021 actatgattc tagaacagca ctccatgtag ctgctgcaga gggtcatgtt gaagttgtta
     4081 aatttttgct ggaagcctgc aaagtaaacc ctttccccaa ggacaggtgg aataacactc
     4141 ccatggatga agcactgcac tttggacacc atgatgtatt taaaattctc caagaatacc
     4201 aagtccagta cacacctcaa ggagattctg acaacgggaa ggaaaatcaa accgtccata
     4261 agaatcttga tggattgttg aagggtcaag acaattctgc agatatccag cacagtggcg
     4321 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     4381 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa actccaaagc
     4441 atcgatggta gcggccgcgg tgaattccgc ccctctccct cccccccccc taacgttact
     4501 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     4561 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     4621 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     4681 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     4741 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4801 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4861 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4921 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     4981 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5041 taagcttgcc acaaccatgg ctgaacaaga tggattgcac gcaggttctc cggccgcttg
     5101 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5161 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5221 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5281 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5341 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5401 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5461 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5521 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5581 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5641 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5701 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5761 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5821 cttctatcgc cttcttgacg agttcttctg agcgggatcg gctagagtcg acccggacgg
     5881 gacgctcctg cgcctgatac agaacgaatt gcttgcaggc atctcatgag tgtgtcttcc
     5941 cgttttccgc ctgaggtcac tgcgtggatg gagcgctggc gcctgctgcg cgacggcgag
     6001 ctgctcacca cccactcgag ggctgcagcg ctgcagaggc cgagtgcaga actgctccaa
     6061 agggacctca aggctttccg agggacacta ggctgactcc atcgagccag tgtagagata
     6121 agcttatcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     6181 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     6241 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     6301 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     6361 cagatcaagg tcaggaacag atggaacagg gtcgacccta gagaaccatc agatgtttcc
     6421 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
     6481 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
     6541 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
     6601 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
     6661 tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc
     6721 cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg
     6781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6901 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tagatccggc tgtggaatgt
     6961 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7021 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7081 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7141 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7201 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7261 cttttttgga ggcctaggct tttgcaaaaa gcttactggc ttaactatgc ggcatcagag
     7321 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7381 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8641 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8941 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     9361 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt agcttggcca
     9421 ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta
     9481 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     9541 gttcatagcc catatatgga gt
//
