LOCUS       sequence-21372-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Isakoff SJ, Engelman JA, Irie HY, Luo J, Brachmann SM, Pearline RV, 
            Cantley LC, Brugge JS
  TITLE     Breast cancer-associated PIK3CA mutations are oncogenic in mammary 
            epithelial cells.
  JOURNAL   Cancer Res. 2005 Dec 1. 65(23):10992-1000.
  PUBMED    16322248
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2005 Dec 1. 65(23):10992-1000."
COMMENT     Sequence Label: JP1520 PIK3CA WT HA
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(111..144)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccaagcttcg ataacttcgt
      121 atagcataca ttatacgaag ttatcagtcg acggtaccgg acatatgccc gggaattcct
      181 gcaggatccg ctcgagaagc ttggtaccga gctcggatct cgagatgcct ccaagaccat
      241 catcaggtga actgtggggc atccacttga tgcccccaag aatcctagta gaatgtttac
      301 taccaaatgg gatgatagtg actttagaat gcctccgtga ggctacgtta ataacgataa
      361 agcatgaact atttaaagaa gcaagaaaat accctctcca tcaacttctt caagatgaat
      421 cttcttacat tttcgtaagt gttacccaag aagcagaaag ggaagaattt tttgatgaaa
      481 caagacgact ttgtgacctt cggctttttc aacccttttt aaaagtaatt gaaccagtag
      541 gcaaccgtga agaaaagatc ctcaatcgag aaattggttt tgctatcggc atgccagtgt
      601 gtgaattcga tatggttaaa gatccagaag tacaggactt ccgaagaaat attctcaatg
      661 tttgtaaaga agctgtggat cttagggatc ttaattcacc tcatagtaga gcaatgtatg
      721 tttatcctcc aaatgtagaa tcttcaccag aactgccaaa gcacatatat aataaattgg
      781 ataaagggca aataatagtg gtgatttggg taatagtttc tccaaataat gacaaacaga
      841 agtatactct gaaaatcaac catgactgtg tgccagaaca agtaattgct gaagcaatca
      901 ggaaaaaaac tcgaagtatg ttgctatcat ctgaacaact aaaactctgt gttttagaat
      961 atc
//
