LOCUS       sequence-213727-        8082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 1-956).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 1-956
FEATURES             Location/Qualifiers
     source          1..8082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3465..3485)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3465..3483)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3504..3520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3504..3520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3517..3539)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3528..3544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3552..3558,3559..3576,3577..3582))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3597..3618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3735..3752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3906..4494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3986..4005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4665..5456,5457..5525))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5288..5307
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5526..5630)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5667..6170
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     6211..6229
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6267..6289)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6389..6408
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6429..6709
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6710..7384
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7487..7942)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7624..7645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7836..7855
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(8068..8082,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(8082,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgag tcatccgatt caatttgtaa acgctaataa
      361 tagcgataag agccaccaat taggcggtca atattctatt cctcaggatt tgagggaaaa
      421 cctgcagaaa gaagctgcac gtattggtga gaacgaaaaa gatgtgctac aggaaaaaat
      481 ggaaactagg acagtgcaaa atcgagagga ttcataccac aaaagaaggt ttgatatgaa
      541 gtttgagccg gattccgata cacaaactgt tacgtcttca gaaaataccc aagacgctgt
      601 cgtgccaagg aaaagaaaat ctcgttggga tgtaaaaggg tacgagccac ctgatgaatc
      661 ttcaacagcg gtcaaagaaa actctgatag tgctttggtt aatgtagaag gaatccatga
      721 tttgatgttt tttaaacctt ctgaccataa atatttcgcc gacgttatat ccaaaaaacc
      781 catagatgag ttaaataagg atgaaaagaa agaaagaacc ctgtcgatgt tacttctgaa
      841 aataaaaaat ggcaatacag cgagtaggag gacttctatg cggattttaa ccgataaagc
      901 tgttacattt ggtccagaaa tgatatttaa ccgcttattg cccatattac tagacagaag
      961 tttggaagac caggaaagac atctaatgat caagaccata gatcgcgtcc tttaccagct
     1021 aggagattta acaaaacctt acgttcacaa aatacttgta gtagctgccc cattgctaat
     1081 agatgaagat ccaatggttc gttcaacagg ccaggagata atcaccaatc tgtctacagt
     1141 cgctggccta aaaaccattc taacagtaat gagacctgat attgagaatg aggatgagta
     1201 tgttagaaat gtaacttcaa gagcggcggc tgtggtggca aaagcccttg gtgttaatca
     1261 attgcttcct ttcatcaatg cagcatgcca ctcacggaag tcttggaaag caaggcatac
     1321 tggcataaaa attgtgcagc aaataggtat acttttggga ataggtgtct tgaatcatct
     1381 tactgggctg atgagttgta tcaaagactg cctaatggat gatcacgttc cggtgcgaat
     1441 tgttacagca catactttat caacactagc agaaaattct tatccatatg gtatcgaagt
     1501 tttcaacgtt gtactggagc cattatggaa gggtataaga agtcatcgtg gtaaagtgct
     1561 atcttctttt ttaaaagctg ttggatctat gatcccattg atggacccag agtatgccgg
     1621 ttattatacc acagaagcaa tgagaataat tagacgtgaa ttcgactctc ccgatgatga
     1681 aatgaaaaag accattcttt tagtattaca aaaatgtagc gctgtagaat ccataacgcc
     1741 aaaatttctg agagaagaaa tcgcgccaga attctttcaa aaattttggg tcagacgagt
     1801 ggcattggat agaccattaa acaaagttgt tacatatact actgttacat tagcaaagaa
     1861 attaggttgt tcttatacca ttgataaatt attaacacca ttgagagatg aagctgagcc
     1921 gttcagaaca atggctgtcc atgctgttac tagaacagta aacttattag ggacagcaga
     1981 tttagatgag agattagaaa cgaggcttat tgatgcgctc ttgatagcat ttcaagaaca
     2041 aacaaatagt gattctataa tctttaaagg gtttggggca gtgacggtgt cacttgacat
     2101 ccgtatgaaa ccgtttttag cacccattgt gagtacgatt ttaaatcatt taaagcataa
     2161 aactcccttg gttcgtcaac atgcagcaga tctatgtgcc atactaatac cagtcatcaa
     2221 gaactgtcac gaattcgaaa tgttgaataa attgaacatt atattgtatg aatccttagg
     2281 cgaagtatat ccagaggtat taggttctat aatcaacgct atgtactgta ttactagcgt
     2341 aatggatttg gataaactac aacctcctat taatcaaatt ttaccaacct taactccaat
     2401 tttaagaaac aagcatagaa aagtcgaagt aaacactatc aaatttgttg gacttattgg
     2461 taaattagca cctacctatg cacctcctaa agaatggatg agaatatgtt ttgaactatt
     2521 agaacttttg aaaagtacaa ataaagaaat aagaagatca gcaaatgcca cgtttggatt
     2581 cattgctgaa gcaattggac ctcacgatgt tcttgttgcg ttattgaaca atttaaaggt
     2641 tcaagaacgt cagttacgtg tatgcacggc tgttgctatc ggtatcgttg ctaaagtgtg
     2701 tggtccttac aacgtgctgc cagtaatcat gaatgaatac acaacaccag agactaatgt
     2761 tcaaaacggt gttctcaagg ccatgtcatt catgtttgag tacattggta acatgtctaa
     2821 ggattacata tacttcataa caccattatt agaagatgca ctcaccgata gggatttagt
     2881 tcatcgtcag acagcgtcga acgtaataac ccacttagct ttaaactgtt caggtacagg
     2941 tcacgaagat gccttcattc atttaatgaa tcttctaata cccaatattt ttgaaacttc
     3001 accacatgct attatgcgta ttttagaagg tttggaggca ctgagtcaag ctcttggccc
     3061 tggactattc atgaactaca tatgggcagg attgttccat ccagcgaaga atgtaaggaa
     3121 agcattttgg agggtgtata ataacatgta cgtaatgtac caggacgcca tggtaccttt
     3181 ttaccccgtt acatgaatat atatagagca tataaataac cacaacttgt aagaatattt
     3241 tacttgacac acctatatca tcacgtcacg atgccttcat agattatgat gaaatataaa
     3301 cagaacctgc tattggaaaa aaaagggcag gaaaaaattt tgtgaaagtg agtaaaagaa
     3361 agatgaagag cgagacatgg ataataagaa atagatgtca ctaatggaca attggaagac
     3421 tgatatggaa agttacgatg aaggaggagc tccagctttt gttcccttta gtgagggtta
     3481 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3541 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3601 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3661 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3721 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3781 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3841 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3901 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3961 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4021 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4081 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4141 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4201 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4261 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4321 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4381 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4441 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4501 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4561 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4621 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4681 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4741 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4801 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4861 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4921 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4981 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5041 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5101 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5161 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5221 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5281 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5341 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5401 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5461 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5521 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5581 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5641 cgaaaagtgc cacctgggtc cttttcatca cgtgctataa aaataattat aatttaaatt
     5701 ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata
     5761 gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt
     5821 atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa
     5881 gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct
     5941 atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt
     6001 ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta
     6061 ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt
     6121 attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc cgtctaagaa
     6181 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6241 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6301 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6361 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6421 catagatcac gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta
     6481 atatatgtgt actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg
     6541 aaaaatagct tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt
     6601 aagaattaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact
     6661 attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat
     6721 taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc
     6781 cgcagaatgt gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag
     6841 aaagagaaca attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa
     6901 aaatagttca ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga
     6961 tgttttggct ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc
     7021 gtggcaagaa taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa
     7081 agactgcaac atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt
     7141 tgattcagaa gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt
     7201 tggaaggcaa gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga
     7261 aaatgttggt gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt
     7321 ggagacaaat ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa
     7381 ataggttatt actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg
     7441 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     7501 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     7561 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     7621 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     7681 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     7741 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     7801 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     7861 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     7921 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     7981 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     8041 gattaagttg ggtaacgcca gggttttccc agtcacgacg tt
//
