LOCUS       sequence-213738-        7422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 237-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 237-972
FEATURES             Location/Qualifiers
     source          1..7422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2805..2825)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2805..2823)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2844..2860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2844..2860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2857..2879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2868..2884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2892..2898,2899..2916,2917..2922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2937..2958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3075..3092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3246..3834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3326..3345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4005..4796,4797..4865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4628..4647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4866..4970)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5007..5510
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5551..5569
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5607..5629)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5729..5748
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5769..6049
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6050..6724
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6827..7282)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6964..6985)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7176..7195
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7408..7422,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7422,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgaa accttacgtt cacaaaatac ttgtagtagc
      361 tgccccattg ctaatagatg aagatccaat ggttcgttca acaggccagg agataatcac
      421 caatctgtct acagtcgctg gcctaaaaac cattctaaca gtaatgagac ctgatattga
      481 gaatgaggat gagtatgtta gaaatgtaac ttcaagagcg gcggctgtgg tggcaaaagc
      541 ccttggtgtt aatcaattgc ttcctttcat caatgcagca tgccactcac ggaagtcttg
      601 gaaagcaagg catactggca taaaaattgt gcagcaaata ggtatacttt tgggaatagg
      661 tgtcttgaat catcttactg ggctgatgag ttgtatcaaa gactgcctaa tggatgatca
      721 cgttccggtg cgaattgtta cagcacatac tttatcaaca ctagcagaaa attcttatcc
      781 atatggtatc gaagttttca acgttgtact ggagccatta tggaagggta taagaagtca
      841 tcgtggtaaa gtgctatctt cttttttaaa agctgttgga tctatgatcc cattgatgga
      901 cccagagtat gccggttatt ataccacaga agcaatgaga ataattagac gtgaattcga
      961 ctctcccgat gatgaaatga aaaagaccat tcttttagta ttacaaaaat gtagcgctgt
     1021 agaatccata acgccaaaat ttctgagaga agaaatcgcg ccagaattct ttcaaaaatt
     1081 ttgggtcaga cgagtggcat tggatagacc attaaacaaa gttgttacat atactactgt
     1141 tacattagca aagaaattag gttgttctta taccattgat aaattattaa caccattgag
     1201 agatgaagct gagccgttca gaacaatggc tgtccatgct gttactagaa cagtaaactt
     1261 attagggaca gcagatttag atgagagatt agaaacgagg cttattgatg cgctcttgat
     1321 agcatttcaa gaacaaacaa atagtgattc tataatcttt aaagggtttg gggcagtgac
     1381 ggtgtcactt gacatccgta tgaaaccgtt tttagcaccc attgtgagta cgattttaaa
     1441 tcatttaaag cataaaactc ccttggttcg tcaacatgca gcagatctat gtgccatact
     1501 aataccagtc atcaagaact gtcacgaatt cgaaatgttg aataaattga acattatatt
     1561 gtatgaatcc ttaggcgaag tatatccaga ggtattaggt tctataatca acgctatgta
     1621 ctgtattact agcgtaatgg atttggataa actacaacct cctattaatc aaattttacc
     1681 aaccttaact ccaattttaa gaaacaagca tagaaaagtc gaagtaaaca ctatcaaatt
     1741 tgttggactt attggtaaat tagcacctac ctatgcacct cctaaagaat ggatgagaat
     1801 atgttttgaa ctattagaac ttttgaaaag tacaaataaa gaaataagaa gatcagcaaa
     1861 tgccacgttt ggattcattg ctgaagcaat tggacctcac gatgttcttg ttgcgttatt
     1921 gaacaattta aaggttcaag aacgtcagtt acgtgtatgc acggctgttg ctatcggtat
     1981 cgttgctaaa gtgtgtggtc cttacaacgt gctgccagta atcatgaatg aatacacaac
     2041 accagagact aatgttcaaa acggtgttct caaggccatg tcattcatgt ttgagtacat
     2101 tggtaacatg tctaaggatt acatatactt cataacacca ttattagaag atgcactcac
     2161 cgatagggat ttagttcatc gtcagacagc gtcgaacgta ataacccact tagctttaaa
     2221 ctgttcaggt acaggtcacg aagatgcctt cattcattta atgaatcttc taatacccaa
     2281 tatttttgaa acttcaccac atgctattat gcgtatttta gaaggtttgg aggcactgag
     2341 tcaagctctt ggccctggac tattcatgaa ctacatatgg gcaggattgt tccatccagc
     2401 gaagaatgta aggaaagcat tttggagggt gtataataac atgtacgtaa tgtaccagga
     2461 cgccatggta cctttttacc ccgttacacc agacaacaat gaagaatata tagaagaact
     2521 ggatttagtt ctgtgaatat atatagagca tataaataac cacaacttgt aagaatattt
     2581 tacttgacac acctatatca tcacgtcacg atgccttcat agattatgat gaaatataaa
     2641 cagaacctgc tattggaaaa aaaagggcag gaaaaaattt tgtgaaagtg agtaaaagaa
     2701 agatgaagag cgagacatgg ataataagaa atagatgtca ctaatggaca attggaagac
     2761 tgatatggaa agttacgatg aaggaggagc tccagctttt gttcccttta gtgagggtta
     2821 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2881 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2941 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3001 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3061 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4141 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4981 cgaaaagtgc cacctgggtc cttttcatca cgtgctataa aaataattat aatttaaatt
     5041 ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata
     5101 gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt
     5161 atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa
     5221 gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct
     5281 atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt
     5341 ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta
     5401 ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt
     5461 attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc cgtctaagaa
     5521 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     5581 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     5641 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     5701 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     5761 catagatcac gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta
     5821 atatatgtgt actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg
     5881 aaaaatagct tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt
     5941 aagaattaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact
     6001 attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat
     6061 taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc
     6121 cgcagaatgt gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag
     6181 aaagagaaca attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa
     6241 aaatagttca ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga
     6301 tgttttggct ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc
     6361 gtggcaagaa taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa
     6421 agactgcaac atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt
     6481 tgattcagaa gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt
     6541 tggaaggcaa gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga
     6601 aaatgttggt gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt
     6661 ggagacaaat ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa
     6721 ataggttatt actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg
     6781 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     6841 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     6901 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     6961 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     7021 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     7081 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     7141 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     7201 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     7261 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     7321 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     7381 gattaagttg ggtaacgcca gggttttccc agtcacgacg tt
//
