LOCUS       sequence-213744-        7845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 95-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 96-972
FEATURES             Location/Qualifiers
     source          1..7845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3228..3248)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3228..3246)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3267..3283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3267..3283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3280..3302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3291..3307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3315..3321,3322..3339,3340..3345))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3360..3381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3498..3515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3669..4257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3749..3768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4428..5219,5220..5288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5051..5070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5289..5393)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5430..5933
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5974..5992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6030..6052)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6152..6171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6192..6472
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6473..7147
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7250..7705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7387..7408)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7599..7618
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7831..7845,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7845,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgaa aagaaaatct cgttgggatg taaaagggta
      361 cgagccacct gatgaatctt caacagcggt caaagaaaac tctgatagtg ctttggttaa
      421 tgtagaagga atccatgatt tgatgttttt taaaccttct gaccataaat atttcgccga
      481 cgttatatcc aaaaaaccca tagatgagtt aaataaggat gaaaagaaag aaagaaccct
      541 gtcgatgtta cttctgaaaa taaaaaatgg caatacagcg agtaggagga cttctatgcg
      601 gattttaacc gataaagctg ttacatttgg tccagaaatg atatttaacc gcttattgcc
      661 catattacta gacagaagtt tggaagacca ggaaagacat ctaatgatca agaccataga
      721 tcgcgtcctt taccagctag gagatttaac aaaaccttac gttcacaaaa tacttgtagt
      781 agctgcccca ttgctaatag atgaagatcc aatggttcgt tcaacaggcc aggagataat
      841 caccaatctg tctacagtcg ctggcctaaa aaccattcta acagtaatga gacctgatat
      901 tgagaatgag gatgagtatg ttagaaatgt aacttcaaga gcggcggctg tggtggcaaa
      961 agcccttggt gttaatcaat tgcttccttt catcaatgca gcatgccact cacggaagtc
     1021 ttggaaagca aggcatactg gcataaaaat tgtgcagcaa ataggtatac ttttgggaat
     1081 aggtgtcttg aatcatctta ctgggctgat gagttgtatc aaagactgcc taatggatga
     1141 tcacgttccg gtgcgaattg ttacagcaca tactttatca acactagcag aaaattctta
     1201 tccatatggt atcgaagttt tcaacgttgt actggagcca ttatggaagg gtataagaag
     1261 tcatcgtggt aaagtgctat cttctttttt aaaagctgtt ggatctatga tcccattgat
     1321 ggacccagag tatgccggtt attataccac agaagcaatg agaataatta gacgtgaatt
     1381 cgactctccc gatgatgaaa tgaaaaagac cattctttta gtattacaaa aatgtagcgc
     1441 tgtagaatcc ataacgccaa aatttctgag agaagaaatc gcgccagaat tctttcaaaa
     1501 attttgggtc agacgagtgg cattggatag accattaaac aaagttgtta catatactac
     1561 tgttacatta gcaaagaaat taggttgttc ttataccatt gataaattat taacaccatt
     1621 gagagatgaa gctgagccgt tcagaacaat ggctgtccat gctgttacta gaacagtaaa
     1681 cttattaggg acagcagatt tagatgagag attagaaacg aggcttattg atgcgctctt
     1741 gatagcattt caagaacaaa caaatagtga ttctataatc tttaaagggt ttggggcagt
     1801 gacggtgtca cttgacatcc gtatgaaacc gtttttagca cccattgtga gtacgatttt
     1861 aaatcattta aagcataaaa ctcccttggt tcgtcaacat gcagcagatc tatgtgccat
     1921 actaatacca gtcatcaaga actgtcacga attcgaaatg ttgaataaat tgaacattat
     1981 attgtatgaa tccttaggcg aagtatatcc agaggtatta ggttctataa tcaacgctat
     2041 gtactgtatt actagcgtaa tggatttgga taaactacaa cctcctatta atcaaatttt
     2101 accaacctta actccaattt taagaaacaa gcatagaaaa gtcgaagtaa acactatcaa
     2161 atttgttgga cttattggta aattagcacc tacctatgca cctcctaaag aatggatgag
     2221 aatatgtttt gaactattag aacttttgaa aagtacaaat aaagaaataa gaagatcagc
     2281 aaatgccacg tttggattca ttgctgaagc aattggacct cacgatgttc ttgttgcgtt
     2341 attgaacaat ttaaaggttc aagaacgtca gttacgtgta tgcacggctg ttgctatcgg
     2401 tatcgttgct aaagtgtgtg gtccttacaa cgtgctgcca gtaatcatga atgaatacac
     2461 aacaccagag actaatgttc aaaacggtgt tctcaaggcc atgtcattca tgtttgagta
     2521 cattggtaac atgtctaagg attacatata cttcataaca ccattattag aagatgcact
     2581 caccgatagg gatttagttc atcgtcagac agcgtcgaac gtaataaccc acttagcttt
     2641 aaactgttca ggtacaggtc acgaagatgc cttcattcat ttaatgaatc ttctaatacc
     2701 caatattttt gaaacttcac cacatgctat tatgcgtatt ttagaaggtt tggaggcact
     2761 gagtcaagct cttggccctg gactattcat gaactacata tgggcaggat tgttccatcc
     2821 agcgaagaat gtaaggaaag cattttggag ggtgtataat aacatgtacg taatgtacca
     2881 ggacgccatg gtaccttttt accccgttac accagacaac aatgaagaat atatagaaga
     2941 actggattta gttctgtgaa tatatataga gcatataaat aaccacaact tgtaagaata
     3001 ttttacttga cacacctata tcatcacgtc acgatgcctt catagattat gatgaaatat
     3061 aaacagaacc tgctattgga aaaaaaaggg caggaaaaaa ttttgtgaaa gtgagtaaaa
     3121 gaaagatgaa gagcgagaca tggataataa gaaatagatg tcactaatgg acaattggaa
     3181 gactgatatg gaaagttacg atgaaggagg agctccagct tttgttccct ttagtgaggg
     3241 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3301 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3361 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3421 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3481 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3541 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3601 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3661 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3721 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3781 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3841 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3901 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3961 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4021 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4081 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4141 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4201 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4261 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4321 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4381 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4441 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4501 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4561 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4621 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4681 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4741 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4801 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4861 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4921 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4981 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5041 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5101 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5161 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5221 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5281 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5341 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5401 ccccgaaaag tgccacctgg gtccttttca tcacgtgcta taaaaataat tataatttaa
     5461 attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa
     5521 atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca aatactacct
     5581 tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta
     5641 gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat cgaatgtata
     5701 tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct ttttgttgaa
     5761 attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta ccttctttat
     5821 ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt aaattcccaa
     5881 attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg atccgtctaa
     5941 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     6001 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     6061 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     6181 caccatagat cacgacatta ctatatatat aatataggaa gcatttaata gaacagcatc
     6241 gtaatatatg tgtactttgc agttatgacg ccagatggca gtagtggaag atattcttta
     6301 ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg gagcttttct ttttttgccg
     6361 attaagaatt aattcggtcg aaaaaagaaa aggagagggc caagagggag ggcattggtg
     6421 actattgagc acgtgagtat acgtgattaa gcacacaaag gcagcttgga gtatgtctgt
     6481 tattaatttc acaggtagtt ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga
     6541 ggccgcagaa tgtgctctag attccgatgc tgacttgctg ggtattatat gtgtgcccaa
     6601 tagaaagaga acaattgacc cggttattgc aaggaaaatt tcaagtcttg taaaagcata
     6661 taaaaatagt tcaggcactc cgaaatactt ggttggcgtg tttcgtaatc aacctaagga
     6721 ggatgttttg gctctggtca atgattacgg cattgatatc gtccaactgc atggagatga
     6781 gtcgtggcaa gaataccaag agttcctcgg tttgccagtt attaaaagac tcgtatttcc
     6841 aaaagactgc aacatactac tcagtgcagc ttcacagaaa cctcattcgt ttattccctt
     6901 gtttgattca gaagcaggtg ggacaggtga acttttggat tggaactcga tttctgactg
     6961 ggttggaagg caagagagcc ccgaaagctt acattttatg ttagctggtg gactgacgcc
     7021 agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg taagcggagg
     7081 tgtggagaca aatggtgtaa aagactctaa caaaatagca aatttcgtca aaaatgctaa
     7141 gaaataggtt attactgagt agtatttatt taagtattgt ttgtgcactt gccgatctat
     7201 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     7261 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     7321 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     7381 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     7441 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     7501 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     7561 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     7621 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     7681 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     7741 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     7801 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgtt
//
