LOCUS       sequence-213760-        8459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For construction of spacer and repeat arrays for Sulfolobus 
            islandicus REY154A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Peng W, Feng M, Feng X, Liang YX, She Q
  TITLE     An archaeal CRISPR type III-B system exhibiting distinctive RNA 
            targeting features and mediating dual RNA and DNA interference.
  JOURNAL   Nucleic Acids Res. 2015 Jan;43(1):406-17. doi: 10.1093/nar/gku1302. 
            Epub 2014 Dec 10.
  PUBMED    25505143
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1302"; journalName: "Nucleic Acids Res"; date: 
            "2015-01"; volume: "43"; issue: "1"; pages: "406-17"
COMMENT     Sequence Label: pSe-Rp1
FEATURES             Location/Qualifiers
     source          1..8459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1119..1142
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1270..1287
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3204..3223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3205..3223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3230..3247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3230..3246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3239..3261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3345..3449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3450..3518,3519..4310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3668..3687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4481..5069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4970..4989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5223..5240
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtttcgttag aacttatgag aagaaaggtt ttatcacggt tgacccacga aacgctgatc
       61 tatgggtacc tagaatagaa ctctataaat tatataagga ttattgcatg gataatgggt
      121 ttcctggagt atcactgaaa acgtttgcta acaaactcaa caaatacttt ggaataacaa
      181 gtatgaagaa gtattttgga aaggatccga atggtaaaga aattaggaga aggtgttttg
      241 tggggatcac gattaactat ggggcgttag agttagaaaa attaggaaaa ccacaagaca
      301 tcacgccttc tgtaccacag gagattagga gatttgaaga atgtttgggt ttttcggtag
      361 gaaatatagc atatcaactt tccacaagaa ctgttgtgag agaagaagaa ttattgaaac
      421 tcttcaaaga tcaacacggt gttgttaaga aagtagagga atgtctgcgt gaattgaatt
      481 ttgttgataa tggagatggg acatttagaa aacccactcc agaggaacta tataaagact
      541 ataaatgcac aacaatatgg tatacaagtc aatataatga gttaaagggc aaaacggtga
      601 aagtcaaaga tctggatcca gattttctga tgtgtctaaa gaaattcggg caaatggaac
      661 aggtagatga tgaaacaatt cgaataaaat aacaacacgc ggggttttac agttttttga
      721 aattcaattt ttaatctata atgaccaatt atcggaccga taggacagga aatcatatat
      781 tttttaacac ggtttttggg tgagttttat aggggtaaaa aatagtgtgt ttcctgtcct
      841 atcggtccgt tttttgcgta agccgtgtta atatctgtat agtaatggcg tttttcgaat
      901 ttttttagta agaatatttg aatttatcta tcgcttttct ctctctagag ccgtgctata
      961 atcaaattaa tatataataa tatgtataaa ataaactggc ggtacatagt ggtacattaa
     1021 agtacaattg tgctgcatgc atgttaaaca agttaggtat actatttata aaatagttag
     1081 gtcataaaag tacccgagaa tgaggtgaag cacatatgtg gagccacccg cagttcgaaa
     1141 aagctagcta cgcgtctccg gatgtacaaa gggctaatct actatagaat tgaaagttca
     1201 gcaggtgacg tcacctgcaa cagctaatct actatagaat tgaaagtcga cgctgatcaa
     1261 gcggccgcac atcatcatca ccaccattaa gcaaaatctt ttttttcccg ggtacctact
     1321 ggcgtagttt aacataaggt tttgtaagaa attcatataa atcctcaata tctcatcata
     1381 aattacaaaa tactcataca cacttctacc ctttttgact aaatcgtttt cgagatcaaa
     1441 ttcataatct ctatctatat aaatataagt taaatctgtt gttggaatta cgccatattc
     1501 ctcgtgtaga ttttccccta ttaatgttag tttataaaga ccggctattt tttcactaat
     1561 ttcatttttt cctaaaaatt gctcctttac atttcatcac cttatcctcg ataatcttat
     1621 ttatagttct taatgctgtt aatggattcc ctgcattata aatacttctt ccaatgattt
     1681 cataatccgc tccagcacat actgcatcgc cataacttcc accttgacta cccatacccg
     1741 gagagactat ggtcattttt tcgaagtctc tcctatactg cgttatatga tctaatttag
     1801 tccctccaac tactattcct tttgggctta tctctcttat aacgttttta atatagtctg
     1861 cgaataacgt actccatcct tcatgtgaca ttacggcaac taagtataaa tttttagagt
     1921 ttgcatcaag atatcttttt aattcatcta gagatccctt aacgcctata aaggaatgtg
     1981 ctatgaacga gttggcgaaa gataatcttt caactatgct tttcattatg tatccgatat
     2041 ctgcaagctt aaaatcaaca ataatttcct ccacgtctaa accaattaag agctctctag
     2101 ttttatccac tcctagatct aaaactaaag gtaaaccaac ttttatccca tataactcat
     2161 tttccatctc tttaagaact tgatatgaga gaggtttatc cattgctaat attactctac
     2221 ttttcaacat tcttcaccaa ataatctaga attgacttct tttcattatc cttaagttta
     2281 tcactcttca acaattcatc tagaatttct gaaattttaa atagagagtg taatttgact
     2341 cctagttttt ccaatctttg tgaagcccct tcttgtctat ctatgattac tagtgcgtct
     2401 gaaactttac ctccaccgtt aagaatctcc aatgttgctt tctctatgga tactcctgta
     2461 gttgcaacgt catctactaa caatactctt tttcctttta catcgagttc taatgtacga
     2521 ttagttccat gacctttctt ttctattcta atatatccca taggctcttt aaggttacaa
     2581 gctatgaatg ccgataaggg aactcctcca gtggctattc ctactattat atcatggggt
     2641 atatcttttg ctttctttat agcttgatta actatatcgt aaaattctgg ataatttggt
     2701 aaaggtctta agtctaagta atatggacta accttacctg atgttaaaac gaaacttcct
     2761 attaataata atttcctttc gagtaagact tctgcgaaat tcatacgtag agactctgtg
     2821 aaaaagaatt taaatatact tctatcataa ccagttataa gggctttgtg agattaagac
     2881 acgtagtttc gtcgcttgac ttgaccagag atgactactt tagaatattc gaacttgcag
     2941 acaagttcta tgatgtaaaa aaactaaatt atctatcagg gaaagtagtt tcattagcat
     3001 tctttgagcc aagtactaga actgctcaaa gctttcatac tgcagcaata aaattaggtg
     3061 ctgatgtgat aggatttgca tccgaggagt ctacttcgat agcaaaaggt gaaaatttgg
     3121 ctgataccat taggatgcta aacaactatt caaactgtat tgtaatgaga cataagtttg
     3181 atggggtacc gagctcgaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     3241 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     3301 atcccccttt cgccaggtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3421 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3481 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3541 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3601 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3661 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3721 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3781 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3841 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3901 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3961 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4021 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4081 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4141 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4201 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4261 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4321 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4381 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4621 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4861 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5101 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5161 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5221 gcagcgagtc agtgagcgag gtaactctct tatacattag tacaagttcg ccgttatttt
     5281 gttcaacact taacaagaat tcgtctcctt ttttcagacc taacgctttt acaatttctc
     5341 gtgggacatt cagataaaac gtaagttcac cacgcccgcc cttttttgcg gatagtctta
     5401 ccataattat attttccgtt caccattttt taaaactttt ccgttcacca aaagctttaa
     5461 atatcacggg gggcaaatct aattttggtg accggaaagt gaacggaaaa ataaacaaaa
     5521 taaacaccct aaataagatt tttttagacc ctgaccccga aaacgtgaaa aacaaaacca
     5581 cggtgaggga actatgaatg cagatgaaat attcagtaac ctagaagaga tattgaacta
     5641 taacagcctg gtgttcatta atagaaagaa tattgaggta gtgtgggcta taaggtctga
     5701 tacagacacg gttcagggat tcgtgagagt tgataacaaa gtattcccat tcaaagcgtg
     5761 ggtagaattt gaaggagagt taagagtgca aatcggcaat ttaatccact tcataataga
     5821 ctccaaaacc gtggaaaaag caatacagag ggagtctgaa taacacgcgg taataccatg
     5881 tttcaaggta ctactttttc ccaatctcta cgcgggtaat acgcaaaatt aacaccaaga
     5941 ggaatttaac ttatttccac tatattgtcc atggtggtta aatggtaagc acgaaaccaa
     6001 atgtgcatat tagattaaga gaagaggaaa ggaaattgtt gaaggaaata gcacaaaagt
     6061 atgatatttc cgaatctgat gtggtaaaga tagcactgaa gaagttagca cgtgagttag
     6121 gaatggataa ttcaccatga tttgaggtga aaacaaggag aaattattca tgatttgacg
     6181 aaaaaaacac agaaaaaggg gggagggctc tatgagtaac ttctcaaatc aagataaaaa
     6241 gcgtttcgcc gaatggttta tcgaacaagg ctttgccatt ttccctattg atccgcaatc
     6301 gaaaaagccc gtgatttcca attggcaaaa atatagccat gagccactta ccgatgaaga
     6361 gaagaagaga ttcttagaac aaattgaaaa gggctataat tatgctgtgc cgggtggtca
     6421 gcagaacctg gtaatcttag attttgagag taaggagtta ttggaaaaat ggataagcaa
     6481 atcggcactt gatgaactgt gcagaaagac cctatgtgtt aacacgcccc acggcgggat
     6541 ccatgtttat gtccaggctg acaatatacc agagcacaag tttaatcccg cctttacgct
     6601 aaacggcaaa ggaatagcgg atttgcagag ttacaatagt tacgtgttgg gcgtgggttc
     6661 atgtataaac cacaagttct gtgatagtga caaatgtaag tggcggggac agaatcatac
     6721 cacgtgttat tcattatata ataataatga attaaagata ggtagagctg atctaaaggg
     6781 actacttaag ttcttagctg aaaaaggaaa agagttaggg atagaattat catctaacgc
     6841 acgggcgtgg ttagaaggag agaaagagga gactgatgtt gaaaaagacc tggagaagtt
     6901 gaagaaggaa ctatccaggt tcaacaagtt taaaggcaaa accatagagg cgataagatc
     6961 tgaagtctgt caaagcatta agaagagtct ggaaaacgta aagagtgaca aagcaaaaac
     7021 catgcttaac acggctttcc aggttgtttg tcagggtaag agttatgggg agatacaatt
     7081 ggataggagt aggggtgatt ggcacgtgtt gaaagtactg ttaagccatg gagtcacgga
     7141 tgttgaagta ttgaaacaac tcttaccaca ggattccaaa gtctttgccc caaaatggga
     7201 ccgatacttt ttacacacgt taatgaaagc ctggaatgaa gtaaaaccgt ttctccaaat
     7261 cataaagaat gccaaaaaca agaagaccaa agaactcaaa caagaattgg ctgaagttct
     7321 ctcacaatat atcataagga aatatcatat tgtcacattc attcaaaaac atagtaatgg
     7381 tgaatcaatt ataggaatct tcagatggaa taggaaaaag ggaatctatg aaccaattga
     7441 tgaaacactt aagaagatta ttaggcatga aatcatgcgt gttattgaga cattccctaa
     7501 atcagaagat gagaaatcac ccatgttcta tgaagtaaga aatgaacttg tcaaactagt
     7561 gtatgatgaa attagggacc taacactcac agaatatgat gatgataaca cgcctcttag
     7621 aatagctttc gaaaactgca cactggaatg gacatcagac aagttcaaac tgattcctgc
     7681 agataaacgc acagaagaac attatgcgtt tcattatgtc ccgcacaaga tccgtgttga
     7741 agtatttaac aacacgccgt accaggtccc agaattagaa gagttagccc gaaaattgtg
     7801 cccaaaatca cttaatgcct ttaaacagtg ggtaggagag aaatgggtca ctttgtttga
     7861 gataatagga tatacattat accccgccac aaaattcaag ttagccttca tgctcttagg
     7921 accccgtgat tccggcaaat caacattcct acaactgttg aagaggattc taggtaaaca
     7981 aaacacggtt agtattaggc taagggaatt gtttgaccca aataacaggt ttgtggcggg
     8041 gtttctattc cacaaacttg tgaacttaac ggcggaaact aaggaataca ccatagagga
     8101 tattgacagg tttaagacat taacgggcgg ggaccaaatc acaagtgatg ttaagttcaa
     8161 aggacctatc acgtttactc cttacgctaa actaatcata gcctctaaca agttaccaga
     8221 tataagagat aagaatgaca cggctttttg gagaaggtgg ctaattatag agtttccaaa
     8281 tcaattcccg aatgatgata actggttcag gcaaacgttt acagaagagg aaatagaggg
     8341 catattgaca gtctcaatat tggcgtttgc ccgtgttatg agtagagggc aatttgacta
     8401 ccaacagact ccagaagaag taagggacct atggctctat aatatagaca gtgtatgga
//
