LOCUS       sequence-213769-        7103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Sertic S, Pizzi S, Cloney R, Lehmann AR, Marini F, Plevani P, 
            Muzi-Falconi M
  TITLE     Human exonuclease 1 connects nucleotide excision repair (NER) 
            processing with checkpoint activation in response to UV irradiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13647-52. doi: 
            10.1073/pnas.1108547108. Epub 2011 Aug 1.
  PUBMED    21808022
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1108547108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-08-16- 16"; volume: "108"; issue: "33"; pages: 
            "13647-52"
COMMENT     Sequence Label: pmCherry-EXO1a
FEATURES             Location/Qualifiers
     source          1..7103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2688..2697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2694..3404
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2839..2857)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3107..3126
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3527..3648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3564..3583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3618..3637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3655..4110)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3792..3813)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4004..4023
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4137..4241
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4243..4600
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4243..4434
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4266..4286)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4451..4586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4635..5429
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4635..5429
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4689..4708)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5299..5318
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5617..5636)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5661..5708
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6037..6625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6526..6545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6800..7103
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttgcttcga tggggataca gggattgcta caatttatca
      301 aagaagcttc agaacccatc catgtgagga agtataaagg gcaggtagta gctgtggata
      361 catattgctg gcttcacaaa ggagctattg cttgtgctga aaaactagcc aaaggtgaac
      421 ctactgatag gtatgtagga ttttgtatga aatttgtaaa tatgttacta tctcacggga
      481 tcaagcctat tctcgtattt gatggatgta ctttaccttc taaaaaggaa gtagagagat
      541 ctagaagaga aagacgacaa gccaatcttc ttaagggaaa gcaacttctt cgtgagggga
      601 aagtctcgga agctcgagag tgtttcaccc ggtctatcaa tatcacacat gccatggccc
      661 acaaagtaat taaagctgcc cggtctcagg gggtagattg cctcgtggct ccctatgaag
      721 ctgatgcgca gttggcctat cttaacaaag cgggaattgt gcaagccata attacagagg
      781 actcggatct cctagctttt ggctgtaaaa aggtaatttt aaagatggac cagtttggaa
      841 atggacttga aattgatcaa gctcggctag gaatgtgcag acagcttggg gatgtattca
      901 cggaagagaa gtttcgttac atgtgtattc tttcaggttg tgactacctg tcatcactgc
      961 gtgggattgg attagcaaag gcatgcaaag tcctaagact agccaataat ccagatatag
     1021 taaaggttat caagaaaatt ggacattatc tcaagatgaa tatcacggta ccagaggatt
     1081 acatcaacgg gtttattcgg gccaacaata ccttcctcta tcagctagtt tttgatccca
     1141 tcaaaaggaa acttattcct ctgaacgcct atgaagatga tgttgatcct gaaacactaa
     1201 gctacgctgg gcaatatgtt gatgattcca tagctcttca aatagcactt ggaaataaag
     1261 atataaatac ttttgaacag atcgatgact acaatccaga cactgctatg cctgcccatt
     1321 caagaagtcg tagttgggat gacaaaacat gtcaaaagtc agctaatgtt agcagcattt
     1381 ggcataggaa ttactctccc agaccagagt cgggtactgt ttcagatgcc ccacaattga
     1441 aggaaaatcc aagtactgtg ggagtggaac gagtgattag tactaaaggg ttaaatctcc
     1501 caaggaaatc atccattgtg aaaagaccaa gaagtgcaga gctgtcagaa gatgacctgt
     1561 tgagtcagta ttctctttca tttacgaaga agaccaagaa aaatagctct gaaggcaata
     1621 aatcattgag cttttctgaa gtgtttgtgc ctgacctggt aaatggacct actaacaaaa
     1681 agagtgtaag cactccacct aggacgagaa ataaatttgc aacattttta caaaggaaaa
     1741 atgaagaaag tggtgcagtt gtggttccag ggaccagaag caggtttttt tgcagttcag
     1801 attctactga ctgtgtatca aacaaagtga gcatccagcc tctggatgaa actgctgtca
     1861 cagataaaga gaacaatctg catgaatcag agtatggaga ccaagaaggc aagagactgg
     1921 ttgacacaga tgtagcacgt aattcaagtg atgacattcc gaataatcat attccaggtg
     1981 atcatattcc agacaaggca acagtgttta cagatgaaga gtcctactct tttaagagca
     2041 gcaaatttac aaggaccatt tcaccaccca ctttgggaac actaagaagt tgttttagtt
     2101 ggtctggagg tcttggagat ttttcaagaa cgccgagccc ctctccaagc acagcattgc
     2161 agcagttccg aagaaagagc gattccccca cctctttgcc tgagaataat atgtctgatg
     2221 tgtcgcagtt aaagagcgag gagtccagtg acgatgagtc tcatccctta cgagaagggg
     2281 catgttcttc acagtcccag gaaagtggag aattctcact gcagagttca aatgcatcaa
     2341 agctttctca gtgctctagt aaggactctg attcagagga atctgattgc aatattaagt
     2401 tacttgacag tcaaagtgac cagacctcca agctatgttt atctcatttc tcaaaaaaag
     2461 acacacctct aaggaacaag gttcctgggc tatataagtc cagttctgca gactctcttt
     2521 ctacaaccaa gatcaaacct ctaggacctg ccagagccag tgggctgagc aagaagccgg
     2581 caagcatcca gaagagaaag catcataatg ccgagaacaa gccggggtta cagatcaaac
     2641 tcaatgagct ctggaaaaac tttggattta aaaaattccg ggatcccgcc accatggtga
     2701 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     2761 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     2821 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     2881 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     2941 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3001 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     3061 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     3121 agaagactat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     3181 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     3241 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     3301 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     3361 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta
     3421 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3481 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     3661 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3721 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3781 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3841 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3901 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3961 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4021 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4081 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     4141 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4201 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     4261 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4321 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4381 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4561 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     4621 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4681 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4741 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4801 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4861 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4921 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4981 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5041 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5101 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5161 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5221 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5281 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5341 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5401 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5461 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5521 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5581 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     5641 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     5701 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     5761 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     5821 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     5881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6721 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     6781 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     6841 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     6901 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     6961 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     7021 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     7081 tattagtcat cgctattacc atg
//
