LOCUS       sequence-213793-        7232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Sertic S, Mollica A, Campus I, Roma S, Tumini E, Aguilera A, 
            Muzi-Falconi M
  TITLE     Coordinated Activity of Y Family TLS Polymerases and EXO1 Protects 
            Non-S Phase Cells from UV-Induced Cytotoxic Lesions.
  JOURNAL   Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi: 
            10.1016/j.molcel.2018.02.017. Epub 2018 Mar 15.
  PUBMED    29551515
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.02.017"; journalName: "Mol Cell"; date: 
            "2018-04-5- 5"; volume: "70"; issue: "1"; pages: "34-47.e4"
COMMENT     Sequence Label: pmCherry-N1-EXO1b
FEATURES             Location/Qualifiers
     source          1..7232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        20..323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        324..527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     478..498
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3140..3149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3146..3856
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3291..3309)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3559..3578
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3979..4100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4016..4035)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4070..4089
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4107..4562)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4244..4265)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4456..4475
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4589..4693
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4695..5052
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4695..4886
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4718..4738)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4903..5038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5087..5881
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5087..5881
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5141..5160)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5751..5770
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6069..6088)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6113..6160
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6489..7077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6978..6997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
       61 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      121 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      181 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      241 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      301 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      361 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      421 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      481 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      541 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttgctt cgatggggat
      601 acagggattg ctacaattta tcaaagaagc ttcagaaccc atccatgtga ggaagtataa
      661 agggcaggta gtagctgtgg atacatattg ctggcttcac aaaggagcta ttgcttgtgc
      721 tgaaaaacta gccaaaggtg aacctactga taggtatgta ggattttgta tgaaatttgt
      781 aaatatgtta ctatctcatg ggatcaagcc tattctcgta tttgatggat gtactttacc
      841 ttctaaaaag gaagtagaga gatctagaag agaaagacga caagccaatc ttcttaaggg
      901 aaagcaactt cttcgtgagg ggaaagtctc ggaagctcga gagtgtttca cccggtctat
      961 caatatcaca catgccatgg cccacaaagt aattaaagct gcccggtctc agggggtaga
     1021 ttgcctcgtg gctccctatg aagctgatgc gcagttggcc tatcttaaca aagcgggaat
     1081 tgtgcaagcc ataattacag aggactcgga tctcctagct tttggctgta aaaaggtaat
     1141 tttaaagatg gaccagtttg gaaatggact tgaaattgat caagctcggc taggaatgtg
     1201 cagacagctt ggggatgtat tcacggaaga gaagtttcgt tacatgtgta ttctttcagg
     1261 ttgtgactac ctgtcatcac tgcgtgggat tggattagca aaggcatgca aagtcctaag
     1321 actagccaat aatccagata tagtaaaggt tatcaagaaa attggacatt atctcaagat
     1381 gaatatcacg gtaccagagg attacatcaa cgggtttatt cgggccaaca ataccttcct
     1441 ctatcagcta gtttttgatc ccatcaaaag gaaacttatt cctctgaacg cctatgaaga
     1501 tgatgttgat cctgaaacac taagctacgc tgggcaatat gttgatgatt ccatagctct
     1561 tcaaatagca cttggaaata aagatataaa tacttttgaa cagatcgatg actacaatcc
     1621 agacactgct atgcctgccc attcaagaag tcgtagttgg gatgacaaaa catgtcaaaa
     1681 gtcagctaat gttagcagca tttggcatag gaattactct cccagaccag agtcgggtac
     1741 tgtttcagat gccccacaat tgaaggaaaa tccaagtact gtgggagtgg aacgagtgat
     1801 tagtactaaa gggttaaatc tcccaaggaa atcatccatt gtgaaaagac caagaagtgc
     1861 agagctgtca gaagatgacc tgttgagtca gtattctctt tcatttacga agaagaccaa
     1921 gaaaaatagc tctgaaggca ataaatcatt gagcttttct gaagtgtttg tgcctgacct
     1981 ggtaaatgga cctactaaca aaaagagtgt aagcactcca cctaggacga gaaataaatt
     2041 tgcaacattt ttacaaagga aaaatgaaga aagtggtgca gttgtggttc cagggaccag
     2101 aagcaggttt ttttgcagtt cagattctac tgactgtgta tcaaacaaag tgagcatcca
     2161 gcctctggat gaaactgctg tcacagataa agagaacaat ctgcatgaat cagagtatgg
     2221 agaccaagaa ggcaagagac tggttgacac agatgtagca cgtaattcaa gtgatgacat
     2281 tccgaataat catattccag gtgatcatat tccagacaag gcaacagtgt ttacagatga
     2341 agagtcctac tcttttaaga gcagcaaatt tacaaggacc atttcaccac ccactttggg
     2401 aacactaaga agttgtttta gttggtctgg aggtcttgga gatttttcaa gaacgccgag
     2461 cccctctcca agcacagcat tgcagcagtt ccgaagaaag agcgattccc ccacctcttt
     2521 gcctgagaat aatatgtctg atgtgtcgca gttaaagagc gaggagtcca gtgacgatga
     2581 gtctcatccc ttacgagaag gggcatgttc ttcacagtcc caggaaagtg gagaattctc
     2641 actgcagagt tcaaatgcat caaagctttc tcagtgctct agtaaggact ctgattcaga
     2701 ggaatctgat tgcaatatta agttacttga cagtcaaagt gaccagacct ccaagctatg
     2761 tttatctcat ttctcaaaaa aagacacacc tctaaggaac aaggttcctg ggctatataa
     2821 gtccagttct gcagactctc tttctacaac caagatcaaa cctctaggac ctgccagagc
     2881 cagtgggctg agcaagaagc cggcaagcat ccagaagaga aagcatcata atgccgagaa
     2941 caagccgggg ttacagatca aactcaatga gctctggaaa aactttggat ttaaaaaaga
     3001 ttctgaaaag cttcctcctt gtaagaaacc cctgtcccca gtcagagata acatccaact
     3061 aactccagaa gcggaagagg atatatttaa caaacctgaa tgtggccgtg ttcaaagagc
     3121 aatattccag cgggatcccg ccaccatggt gagcaagggc gaggaggata acatggccat
     3181 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3241 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3301 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3361 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3421 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3481 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     3541 caacttcccc tccgacggcc ccgtaatgca gaagaagact atgggctggg aggcctcctc
     3601 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     3661 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     3721 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     3781 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     3841 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     3901 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3961 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4081 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     4141 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     4201 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     4261 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4321 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     4381 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4441 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4501 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4561 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4621 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4681 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     4741 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4801 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4861 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5041 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5101 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5161 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5221 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     5281 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5341 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5401 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5461 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5521 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5581 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     5641 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5701 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5761 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5821 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5881 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5941 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6001 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     6061 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     6121 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     6181 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     6241 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     6301 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     6361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7141 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
     7201 atagtaatca attacggggt cattagttca ta
//
