LOCUS       sequence-213795-        9401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express tagged Cdc6 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Matson JP, Dumitru R, Coryell P, Baxley RM, Chen W, Twaroski K, 
            Webber BR, Tolar J, Bielinsky AK, Purvis JE, Cook JG
  TITLE     Rapid DNA replication origin licensing protects stem cell 
            pluripotency.
  JOURNAL   Elife. 2017 Nov 17;6. doi: 10.7554/eLife.30473.
  PUBMED    29148972
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-11-17"; volume: "6. doi"; pages: " 10.7554/eLife.30473"
COMMENT     Sequence Label: CLXSN 5myc-CDC6-mut
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             591..766
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    829..1028
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1229..1645
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1575..1597
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1613..1629
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1707..1726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1707..1725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1793..1822
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1826..1855
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1865..1894
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1898..1927
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3639..3659)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3644..3973
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3824..3959
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4033..4827
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4087..4106)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4697..4716
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4887..5480
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     intron          5825..5890
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6020..6040
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6312..6446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6349..6368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6403..6422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6904..7038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7103..7120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7274..7862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7354..7373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8033..8824,8825..8893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8656..8675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8894..8998)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9066..9084
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     9244..9263
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct caataaaaga
      601 gcccacaacc cctcactcgg cgcgccagtc ttccgataga ctgcgtcgcc cgggtacccg
      661 tattcccaat aaagcctctt gctgtttgca tccgaatcgt ggtctcgctg ttccttggga
      721 gggtctcctc tgagtgattg actacccacg acgggggtct ttcatttggg ggctcgtccg
      781 ggatttggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc
      841 aacttatctg tgtctgtccg attgtctagt gtctatgttt gatgttatgc gcctgcgtct
      901 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      961 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
     1021 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
     1081 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
     1141 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1201 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1261 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1321 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1381 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1441 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1501 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1561 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1621 agccctcact ccttctctag gcgccggaat tcgttaactc gactctggct aactagagaa
     1681 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
     1741 taccaccatg gaacagaagc taatctctga ggaagttttg ctggggatct tggagcagaa
     1801 attgataagc gaggaagacc ttggagaaca aaagctgatt tcggaagagg atctggggat
     1861 cttggagcag aaattgataa gcgaggaaga ccttggagaa caaaagctga tttcggaaga
     1921 ggatctgggg atccgaatgc ctcaaacccg atcccaggca caggctacaa tcagttttcc
     1981 aaaaaggaag ctgtctcggg cattgaacaa agctaaaaac tccagtgatg ccaaactaga
     2041 accaacaaat gtccaaaccg taacctgttc tcctcgtgta aaagccctgc ctctcagccc
     2101 cgccaaacgt gccggcgatg acaacctatg caacactccc catttacctc cttgttctcc
     2161 accaaagcaa ggcaaggcgg ccgcaggtcc ccctcactca catacactta agggacgaag
     2221 attggtattt gacaatcagc tgacaattaa gtctcctagc aaaagagaac tagccaaagt
     2281 tcaccaaaac aaaatacttt cttcagttag aaaaagtcaa gagatcacaa caaattctga
     2341 gcagagatgt ccactgaaga aagaatctgc atgtgtgaga ctattcaagc aagaaggcac
     2401 ttgctaccag caagcaaagc tggtcctgaa cacagctgtc ccagatcggc tgcctgccag
     2461 ggaaagggag atggatgtca tcaggaattt cttgagggaa cacatctgtg ggaaaaaagc
     2521 tggaagcctt tacctttctg gtgctcctgg aactggaaaa actgcctgct taagccggat
     2581 tctgcaagac ctcaagaagg aactgaaagg ctttaaaact atcatgctga attgcatgtc
     2641 cttgaggact gcccaggctg tattcccagc tattgctcag gagatttgtc aggaagaggt
     2701 atccaggcca gctgggaagg acatgatgag gaaattggaa aaacatatga ctgcagagaa
     2761 gggccccatg attgtgttgg tattggacga gatggatcaa ctggacagca aaggccagga
     2821 tgtattgtac acgctatttg aatggccatg gctaagcaat tctcacttgg tgctgattgg
     2881 tattgctaat accctggatc tcacagatag aattctacct aggcttcaag ctagagaaaa
     2941 atgtaagcca cagctgttga acttcccacc ttataccaga aatcagatag tcactatttt
     3001 gcaagatcga cttaatcagg tatctagaga tcaggttctg gacaatgctg cagttcaatt
     3061 ctgtgcccgc aaagtctctg ctgtttcagg agatgttcgc aaagcactgg atgtttgcag
     3121 gagagctatt gaaattgtag agtcagatgt caaaagccag actattctca aaccactgtc
     3181 tgaatgtaaa tcaccttctg agcctctgat tcccaagagg gttggtctta ttcacatatc
     3241 ccaagtcatc tcagaagttg atggtaacag gatgaccctg agccaagaag gagcacaaga
     3301 ttccttccct cttcagcaga agatcttggt ttgctctttg atgctcttga tcaggcagtt
     3361 gaaaatcaaa gaggtcactc tggggaagtt atatgaagcc tacagtaaag tctgtcgcaa
     3421 acagcaggtg gcggctgtgg accagtcaga gtgtttgtca ctttcagggc tcttggaagc
     3481 caggggcatt ttaggattaa agagaaacaa ggaaacccgt ttgacaaagg tgtttttcaa
     3541 gattgaagag aaagaaatag aacatgctct gaaagataaa gctttaattg gaaatatctt
     3601 agctactgga ttgccttaag cgtcgaggat ccggctgtgg aatgtgtgtc agttagggtg
     3661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3721 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3781 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3841 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3901 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3961 aggcttttgc aaaaagcttg ggctgcaggt cgaggcggat ctgatcaaga gacaggatga
     4021 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4081 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4141 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4201 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4261 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4321 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4381 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4441 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4501 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4561 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4621 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4681 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4741 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4801 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgataaa ataaaagatt
     4861 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     4921 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     4981 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     5041 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     5101 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     5161 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     5221 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     5281 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     5341 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     5401 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     5461 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     5521 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     5581 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5641 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     5701 acccagtcac gtagcgatag cggagtgtat ctagctaggt agctagagga tctttgtgaa
     5761 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     5821 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     5881 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     5941 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     6001 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     6061 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     6121 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     6181 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     6241 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     6301 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6361 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6421 tcaatgtatc ttatcatgtc tggatcagct tcagaagatg gcggagggcc tccaacacag
     6481 taattttcct cccgactctt aaaatagaaa atgtcaagtc agttaagcag gaagtgacta
     6541 actgaccgca gctggccgtg cgacatcctc ttttaattag ttgctaggca actgccctcc
     6601 agagggcagt gtggttttgc aagaggaagc aaaaagcctc tccacccagg cctagaatgt
     6661 ttccacccaa tcattactat gacaacagct gtttttttta gtattaagca gaggccgggg
     6721 gcccctggcc tccgcttact ctggagaaaa agaagagagg cattgtagag gcttccagag
     6781 gcaacttgtc aaaacaggac tgcttctatt tctgtcacac tgtctggccc tgtcacaagg
     6841 tccagcacct ccataccccc tttaataagc agtttgggaa cgggtgcggg tcttactccg
     6901 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6961 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7021 ggaggctttt ttggaggctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7681 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8341 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9001 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9061 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9121 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9181 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9241 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9301 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     9361 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca g
//
