LOCUS       sequence-213806-        5507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A backbone vector for cell surface display in E. coli by Ligation 
            Independent Cloning (LIC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ko HJ, Park E, Song J, Yang TH, Lee HJ, Kim KH, Choi IG
  TITLE     Functional cell surface display and controlled secretion of diverse 
            Agarolytic enzymes by Escherichia coli with a novel 
            ligation-independent cloning vector based on the autotransporter 
            YfaL.
  JOURNAL   Appl Environ Microbiol. 2012 May;78(9):3051-8. doi: 
            10.1128/AEM.07004-11. Epub 2012 Feb 17.
  PUBMED    22344647
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.07004-11"; journalName: "Appl Environ Microbiol"; date:
            "2012-05"; volume: "78"; issue: "9"; pages: "3051-8"
COMMENT     Sequence Label: pATLIC
FEATURES             Location/Qualifiers
     source          1..5507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..295
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     218..237
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1845..1862
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1918..1935)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2088..2174
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2266..2293
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2312..2403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2404..2472,2473..3264)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2622..2641)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3435..4023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3924..3943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4177..4194
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4209..4349
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4275..4294)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4435..4457
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4613..5491)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEARERYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
       61 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
      121 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
      181 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
      241 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
      301 ttttttggct ttaagaagga gatataagga tgcggattat ctttctacgc aaggagtatt
      361 tatctttact cccgtcaatg attgcatctc ttttctctgc taacggtgtc gcgcccgggc
      421 caacgaccga aaacctttac ttccagcttt ccaacgtgac ggttaatggc aatctgacca
      481 atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg acggtaaacg
      541 gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc gatgactcgg
      601 taggcgatca attggtgatg aacagtaata ctgctggcaa cacaactgtg gtggttaact
      661 ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat ttcgcagctg
      721 atcccacgca gtttcaaaac aatgcgcagt tcagtctggc aggcagcggc tacgtcaata
      781 tgggagcgta tgactacacg ctggtggaag ataacaacga ctggtatctg cgatcgcaag
      841 aagtaacgcc gccatcgcca cctgatccag acccgactcc cgatcctgat cccacgccgg
      901 atcctgatcc aacacccgac ccggaaccta cgcctgctta ccagccggtg ttgaatgcca
      961 aagttggcgg ttatcttaat aacctgcggg cggcaaatca ggcgtttatg atggagcgac
     1021 gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg tgttatcggc ggagattatc
     1081 attacacagc agcggggcaa ctggctcaac atgaagacac ttctacggtg caacttagcg
     1141 gcgatctgtt tagcgggcgc tggggcacgg atggcgagtg gatgcttggg attgttggtg
     1201 gctacagcga taaccagggc gacagccgct cgaatatgac cggaactcgc gccgataacc
     1261 agaaccacgg ttatgccgtt gggctgacat caagctggtt tcagcacggt aatcagaggc
     1321 aaggggcctg gctggatagc tggctgcaat acgcgtggtt tagcaatgat gtttccgaac
     1381 aagaagatgg cacagatcat taccactcgt cggggattat cgcctcgctg gaggcggggt
     1441 atcagtggtt accggggcgt ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg
     1501 gcgtgcagca ggatgatttt accgccgcta accgtgcgcg cgtgtcacaa tcgcagggtg
     1561 atgatattca gacgcggctg ggtttacaca gcgaatggcg taccgctgtt catgtcatac
     1621 caacattaga tctgaattat tatcacgatc cccattcgac ggaaattgaa gaggatggca
     1681 gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat aaaagtggga gtcacgggca
     1741 atatcagtca gcgagtgtcc ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt
     1801 ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg gtaacaccat caccatcatc
     1861 actgagatcc ggctgctaac aaagtggctg ttttggcgga tgagagaaga ttttcagcct
     1921 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
     1981 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
     2041 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
     2101 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     2161 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     2221 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
     2281 cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat
     2341 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2401 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2461 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2521 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     2581 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2641 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2701 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2761 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2821 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2881 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2941 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3001 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3061 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3121 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3181 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3241 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3301 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3361 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3421 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3481 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3541 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3601 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3661 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3721 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3781 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3841 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3901 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3961 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4021 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4081 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4141 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4201 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4261 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     4321 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     4381 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4441 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt
     4501 cgcgcgcgaa ggcgaagcgg catgcataat gtgcctgtca aatggacgaa gcagggattc
     4561 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     4621 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     4681 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     4741 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     4801 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     4861 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     4921 ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga
     4981 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     5041 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     5101 tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg
     5161 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     5221 gttcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     5281 tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     5341 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     5401 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     5461 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccg
//
