LOCUS       sequence-213807-       10840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-tagged Arkadia in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Mavrakis KJ, Andrew RL, Lee KL, Petropoulou C, Dixon JE, Navaratnam 
            N, Norris DP, Episkopou V
  TITLE     Arkadia enhances Nodal/TGF-beta signaling by coupling 
            phospho-Smad2/3 activity and turnover.
  JOURNAL   PLoS Biol. 2007 Mar;5(3):e67. doi: 10.1371/journal.pbio.0050067.
  PUBMED    17341133
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2007-03"; volume: "5(3):e67. doi"; pages: " 
            10.1371/journal.pbio.0050067"
COMMENT     Sequence Label: gfp-Arkadia/RNF111
FEATURES             Location/Qualifiers
     source          1..10840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..828
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1..125
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(163..768)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        960..1263
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1916..1938
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2000..2019
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2061..2080
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2061..2079
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2080..2104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2120..2229
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             2250..2267
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2277..2321
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     regulatory      2349..2358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2355..2357,2358..2360,2361..3071)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2355..2357,2358..2360,2361..3071)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2400..2421)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2661..2680)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3008..3029
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4599..4619
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             6193..6216
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    6270..6732
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6326..6343)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             6771..7790
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             6771..7790
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(7955..7974)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8020..8075
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8074..8093)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      8163..8210
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     8223..8928
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           8311..8683
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(8340..8928)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(8937..8970)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(9138..9725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9217..9236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9738..10529,10530..10598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10361..10380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
       61 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      121 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      181 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      241 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      301 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      361 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      421 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      481 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      541 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      601 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      661 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      721 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      781 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
      841 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
      901 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      961 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1021 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1081 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1141 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1201 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1261 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1321 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1381 gggggggggg nngngsgssr ggsggggngg gggggggggg gggggggggg gggggggggg
     1441 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
     1501 gggggggggg ggggsggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1741 gggacggccc ttctccttcg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1861 gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     1921 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     1981 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     2041 gaattggatc ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa
     2101 ttccccggag ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata
     2161 agaattatta tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta
     2221 tttacaatca aaggagatat accatggcac accatcacca ccatcactct tctggtaaag
     2281 aaaccgctgc tgcgaaattt gaacgccagc acatggactc gccaccgcct tctggtctgg
     2341 tccccctcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2401 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2461 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2521 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     2581 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2641 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2701 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2761 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2821 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2881 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2941 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     3001 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3061 agctgtacaa gtccggactc agatctaagc ttgagctcta caccctaaaa gtggctatga
     3121 agagtgggac tcctgatgcg cccactacac aggagagtct gaaggcggtc cttctgcacc
     3181 cacagcccct aggggccacc aagagcttcc ctgcagaagt ggagatgatt aatagcaaag
     3241 tggggaatga attctctcat ctgtgtgatg attctcaaaa acaagagaag gacatgactg
     3301 gcaaccagca agaacaagaa aaaagtggtg ttgtgagaaa aaagcgcaaa agccagcagg
     3361 ctggcccctc atatgtgcag aactgtgtca aagaaaatca ggaaatccta gggcgcagac
     3421 aacagctaga gacaccgagt gatgaggata atgactcttc cttaagtgag tgtctgtctt
     3481 ctccctcatc tagtctccat tttgggggct ctgatacagt gacttcagat gaggataaag
     3541 aagtctcggt gagacatacc cagcctgttc tgagtgctaa aagtagaagt cacagtgcaa
     3601 ggtctcacaa gtggcctcga actgaggcag atcctgtgcc aagtttgtta atgaaaagac
     3661 cttgttttca tggcagtgca ctaaggagag tcacatgcag aaagaggctg gtgaagagca
     3721 gctcctcgca gaggacacag aagcagaagg agaggatgct agtgcagagg aagaagcggg
     3781 aggcgctagc tcagaggaag tatgctcttc tctccagctc cagtagctcc agtgagaatg
     3841 acctcagcag cgattcctcg tccagctcct ccaccgatgg agaagaggac ctgtgtgcat
     3901 ctgccagtga gaaccccagc aaccccgctg ctccctcagg aagtattgat gaagatgttg
     3961 tggtgataga agcctccttc actccccagg tcactgccaa tgaagaaatt aatgttacct
     4021 caactgacag tgaagtggag attgttacag ttggagaaag ctatcggtct cgttcaaccc
     4081 ttggacactc cagatctcat tggagccaag gttcaagttc gcatacagga cggccacagg
     4141 agtctcgaaa ccgcagtagg atctctactg ttatacagcc cctgaggcag aatgcagcag
     4201 aagttgtgga ccttactgtt gatgaagatg aacctactat agtaccaacc acttctgcta
     4261 gaatggactc acaaactact agtgcttcca ttaacaattc aaatccatct acctctgagc
     4321 aagcctctga tactacttca actgtggcca gtagccagcc ttccacagtg tcagagactg
     4381 aagctactct tacaagcaat agtgcaactg gttcttctgt gggcgatgac gtgaggagaa
     4441 ctgcatctag tgctgtccca gaaagtggtc ctcctgcaat gccaaggcta ccttcttgct
     4501 gcccccagca ctcaccatgt ggagggacat cacagagcca tcatgcatta gcacacccac
     4561 actcaagttg ttttcagcag catggccacc attttcagca tcaccaccat caccaccata
     4621 ctccccaccc agctgtccca gtttctcctt ccttcagtga ccctgcttgc ccagtggaaa
     4681 gacctcaagt acaggcgcct tgtggagcaa atagcagctc tggttccagc taccatgacc
     4741 agcaggcatt gccagtcgat ctgagcaaca gtgctctcag gactcatgga agtgggggtt
     4801 tccatggtgc atctgctttt gacccctgct gccctgtcac atcttcccgg gctgcagtct
     4861 ttggccatca ggctgctgct gccccgactc agccattatc aatagatggc tatggctcca
     4921 gcatggttgc ccagccccag ccccagcccc ctccacagcc ttctctttcg tcatgtcggc
     4981 actacatgcc acctccgtat gcttccttga caagaccact gcaccatcaa gcctcagctt
     5041 gccatcactc tcatggaaat gcccctcccc agactcagcc tccccctcaa gtggattacg
     5101 tgattccgca tcctgtccat gctttccatt ctcagatatc ttctcatgca gcatctcacc
     5161 ctgtggcacc accgccgcca acacatttag gcagtacagc ggcacccatc cctcagcatc
     5221 ttcctcctgc tcaccagccg atttcacacc atattccagc cccagcacct tcagcccaga
     5281 gactgcaccc tcatgaagtg atgcagagga tggaagttca gaggaggagg atgatgcagc
     5341 atccaacgcg ggcacatgaa cggcccccgc cccatccaca taggatgcac ccaaactatg
     5401 gccatgggca tcatattcat gtgcctcaaa ccatgtcttc acatcctcgc caggctccag
     5461 agagaactgc ctgggaactg gggattgaag ctggagtaac tgcagctacc tacacacctg
     5521 gtgctctgca ccctcacctg gcccattacc acgccccgcc tcgacttcat cacctccagt
     5581 taggagcact tcctttaatg gttcctgaca tggcaggcta tcctcacatc cgttatattt
     5641 cttcaggatt ggatggagca tcattcagag gtccttttag gggcaatttt gaggaattga
     5701 ttcatctgga agaaagatta ggaaatgtca atcgtggagc ctcccaggga acaattgaaa
     5761 gatgcacata tccacataaa tataaaaagg taacaactga ttggttctca cagaggaaac
     5821 tgcactgcaa acaagatggg gaagaaggga ctgaggaaga cacagaggaa aaatgtacta
     5881 tctgtttgtc tattttagag gaaggtgaag atgtgaggcg ccttccatgt atgcaccttt
     5941 tccaccaagt gtgtgttgat cagtggctga tcaccaataa gaagtgcccc atatgcagag
     6001 tggacatcga ggcccagctg ccaagtgaaa gttgacattg tgtttcagaa ctcttggtgt
     6061 ccctctcatt cctatccttc ctggtactgc agtcaaccaa agatggcatg acttacctgc
     6121 gcagatctgt catgatgatc attgcaattg gatccatata tagggcccgg gttataatta
     6181 cctcaggtcg agcaccacca tcaccatcac catcactaag tgattaacct caggtgcagg
     6241 ctgcctatca gaaggtggtg gctggtgtgg agggcccgga aacctggccc tgtcttcttg
     6301 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6361 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     6421 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     6481 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6541 gaaagagtca aatggctcac ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6601 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6661 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     6721 acacgatgat aagcttgcca caacccgtac caaagatgga tagatccgga aagcctgaac
     6781 tcaccgcgac gtctgttgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     6841 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     6901 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     6961 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa tttagcgaga
     7021 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     7081 ccgaactgcc cgctgttctg caaccggtcg cggaggctat ggatgcgatc gctgcggccg
     7141 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     7201 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     7261 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     7321 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     7381 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     7441 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     7501 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     7561 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     7621 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     7681 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     7741 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc
     7801 gaccgaacaa gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag
     7861 gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaac
     7921 caatgccctg gctcacaaat accactgaga tcgatctttt tccctctgcc aaaaattatg
     7981 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     8041 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     8101 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgt
     8161 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagcat
     8221 gcggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac
     8281 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat
     8341 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat
     8401 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg
     8461 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta
     8521 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc
     8581 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt
     8641 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt
     8701 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca
     8761 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg
     8821 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg
     8881 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattgc gatcgcataa
     8941 cttcgtatag catacattat acgaagttat aagctcggaa cgctgcgctc ggtcgttcgg
     9001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9121 gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac aaaaatcgac
     9181 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9241 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9301 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9361 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9421 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9481 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9541 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9601 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9661 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9721 ctcaagaaga tcctttgtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9781 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9841 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9901 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9961 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10021 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10081 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10141 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10201 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10261 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10321 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10381 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10441 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10501 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10561 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10621 tgaagcattt atcagggtta ttgtctcatg tccgcgcgtt tcctgcatct tttaatcaaa
    10681 tcccaagatg tgtataaacg cgccggtatg tacaggaaga ggtttatact aaactgttac
    10741 attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat gtttaatcaa ggctctgacg
    10801 catttctaca accacgactc caagtgtgtg ggtgaagtca
//
