LOCUS       sequence-213841-        9940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Beta-estradiol inducible expression vector; Z4 promoter-CYC1 
            terminator; artificial transcription factor under ACT1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Patterson K, Shavarebi F, Magnan C, Chang I, Qi X, Baldi P, 
            Bilanchone V, Sandmeyer SB
  TITLE     Local features determine Ty3 targeting frequency at RNA polymerase 
            III transcription start sites.
  JOURNAL   Genome Res. 2019 Aug;29(8):1298-1309. doi: 10.1101/gr.240861.118. 
            Epub 2019 Jun 27.
  PUBMED    31249062
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.240861.118"; journalName: "Genome Res"; date: "2019-08";
            volume: "29"; issue: "8"; pages: "1298-1309"
COMMENT     Sequence Label: pKP3915
FEATURES             Location/Qualifiers
     source          1..9940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      133..382
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(149..167)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(428..451)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        1175..1518
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1519..2328
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1586..1605)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2955..2971
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2956..2972
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4491..4678
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4546..4570)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     misc_feature    5379..5671
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6111..6564
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(6899..7702)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7703..7923)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7948..7966)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8034..8138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8139..8207,8208..8999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8357..8376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9170..9758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9659..9678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9912..9929
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacttaa ttaagtttaa actccggaca agatggcctt tggtgggttg aagaaggaaa
       61 aagacagaaa cgacttaatt acctacttga aaaaagcctg tgagtaaaca ggcccctttt
      121 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct cctcccacat
      181 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      241 tttaatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
      301 gtacaaacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
      361 acgctcgaag gctttaattt gcaaagctta agagcgctgc gcgctacgta tatagttttt
      421 tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac ttttatgaca
      481 tttgaataag aagtaataca aactgaaaat gttgaaagta ttagttaaag tggttatgca
      541 gcttttccat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc
      601 ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt
      661 taatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag
      721 ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca
      781 ggaacgcgac cggtgaagac gaggacgcac ggtctagact cctccgccgc actcctccgc
      841 cgctcctcct ccgccgcact cctccgccgc tcctcctccg ccgcactcct ccgccgcggc
      901 cgcacttcaa tatagcaatg agcagttaag cgtattactg aaagttccaa agagaaggtt
      961 tttttaggct aagataatgg ggctctttac atttccacaa catataagta agattagata
     1021 tggatatgta tatggatatg tatatggtgg taatgccatg taatatgatt attaaacttc
     1081 tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat tcaatataaa gatctgttta
     1141 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     1201 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     1261 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     1321 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     1381 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     1441 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     1501 atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat
     1561 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1621 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1681 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1741 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1801 ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca
     1861 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1921 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1981 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2041 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     2101 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2161 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2221 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2281 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agtactgaca
     2341 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     2401 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     2461 cagatgcgaa gttaagtgcg ggccggcccg tacgctcacc ctaacatatt ttccaattaa
     2521 ccctcaatat ttctctgtca cccggcctct attttccatt ttcttcttta cccgccacgc
     2581 gtttttttct ttcaaatttt tttcttcctt cttctttttc ttccacgtcc tcttgcataa
     2641 ataaataaac cgttttgaaa ccaaactcgc ctctctctct cctttttgaa atatttttgg
     2701 gtttgtttga tcctttcctt cccaatctct cttgtttaat atatattcat ttatatcacg
     2761 ctctcttttt atcttccttt ttttcctctc tcttgtattc ttccttcccc tttctactca
     2821 aaccaagaag aaaaagaaaa ggtcaatctt tgttaaagaa taggatcttc tactacatca
     2881 gcttttagat ttttcacgct tactgctttt ttcttcccaa gatcgaaaat ttactgaatt
     2941 aacagggccc cccctcgagg tcgacggtat cgataagctt gaagcaagcc tcctgaaaga
     3001 tgggtacccg cccatatgct tgccctgtcg agtcctgcga tcgccgcttt tctcgccacg
     3061 ccaatcttac ccgccatatc cgcatccata ccggtcagaa gcccttccag tgtcgaatct
     3121 gcatgcgtaa cttcagtcgt aatgcgaacc ttgtgcgcca catccgcacc cacacaggat
     3181 cccaaaagcc gttccaatgt cggatctgta tgcggaactt tagtcgaaag gccgacctga
     3241 ggcgtcacat tcgcacgcac accggcgaga agccttttgc ctgtgacatt tgtgggagga
     3301 agtttgccag gaagggcgac ctcaagaggc ataccaaaat ccatacaggt ggcggaggca
     3361 cacctgcagc tgcgtcgact ctagaggatc catctgctgg agacatgaga gctgccaacc
     3421 tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc ttgtccctga
     3481 cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc tattccgagt
     3541 atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc aacctggcag
     3601 acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt gtggatttga
     3661 ccctccatga tcaggtccac cttctagaat gtgcctggct agagatcctg atgattggtc
     3721 tcgtctggcg ctccatggag cacccagtga agctactgtt tgctcctaac ttgctcttgg
     3781 acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg ctgctggcta
     3841 catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc ctcaaatcta
     3901 ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag tctctggaag
     3961 agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc cacctgatgg
     4021 ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc ctcctcatcc
     4081 tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc atgaagtgca
     4141 agaacgtggt gcccctctat gacctgctgc tggagatgct ggacgcccac cgcctacatg
     4201 cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac ttggccactg
     4261 cgggctctac ttcatcggag ctccacttag acggcgagga cgtggcgatg gcgcatgccg
     4321 acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat
     4381 ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc
     4441 agatgtttac cgatgccctt ggaattgacg agtacggtgg gtagtaataa gcgaatttct
     4501 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     4561 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     4621 tcaggttgct ttctcaggta tagcatgaga tcgctcttat tgaccacacc tctaccggca
     4681 tgccgagcaa atgcctgcaa atcgctcccc ctcagcattt aaatcctgca gggaattcct
     4741 ttctgcatgc cacgttcgca gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4801 atttcacacc gcataatcgc tgggccattc tcatgaagaa tatcttgaat ttattgtcat
     4861 attactagtt ggtgtggaag tcctaatatc ggtgatcaat atagtggttg acatgctggc
     4921 tagtcaacat tgagcctttt gatcatgcaa atatattacg gtattttaca atcaaatatc
     4981 aaacttaact attgacttta taacttattt aggtggtaac attcttataa aaaagaaaaa
     5041 aattactgca aaacagtact agcttttaac ttgtatccta ggttatctat gctgtctcac
     5101 catagagaat attacctatt tcagaatgta tgtccatgat tcgccgggta aatacatata
     5161 atacacaaat ctggcttaat aaagtctata atatatctca taaagaagtg ctaaattggc
     5221 tagtgctata tatttttaag aaaatttctt ttgactaagt ccatatcgac tttgtaaaag
     5281 ttcacattag catacatata ttacacgagc cagaaatagt aacttttgcc taaaatcaca
     5341 aattgcaaaa tttaattgct tgcaaaaggt cacatgctta taatcaactt ttttaaaaat
     5401 ttaaaatact tttttatttt ttatttttaa acataaatga aataatttat ttattgttta
     5461 tgattaccga aacataaaac ctgctcaaga aaaagaaact gttttgtcct tggaaaaaaa
     5521 gcactaccta ggagcggcca aaatgccgag gctttcatag cttaaactct ttacagaaaa
     5581 taggcattat agatcagttc gagttttctt attcttcctt ccggttttat cgtcacagtt
     5641 ttacagtaaa taagtatcac ctcttagagt tcgatgataa gctgtcaaac atgagaatta
     5701 attccacatg ttaaaatagt gaaggagcat gttcggcaca cagtggaccg aacgtggggt
     5761 aagatcgatg ataagctgtc aaacattgca ctagggtccg gttaaacgga tctcgcattg
     5821 atgaggcaac gctaattatc aacatataga ttgttatcta tctgcatgaa cacgaaatct
     5881 ttacttgacg acttgaggct gatggtgttt atgcaaagaa accactgtgt ttaatatgtg
     5941 tcactgtttg atattactgt cagcgtagaa gataatagta aaagcggtta ataagtgtat
     6001 ttgagataag tgtgataaag tttttacagc gaaaagacga taaatacaag aaaatgatta
     6061 cgaggatacg gagagaggta tgtacatgtg tatttatata ctaagctgcc ggcggttgtt
     6121 tgcaagaccg agaaaaggct agcaagaatc gggtcattgt agcgtatgcg cctgtgaaca
     6181 ttctcttcaa caagtttgat tccattgcgg tgaaatggta aaagtcaacc ccctgcgatg
     6241 tatattttcc tgtacaatca atcaaaaagc caaatgattt agcattatct ttacatcttg
     6301 ttattttaca gattttatgt ttagatcttt tatgcttgct tttcaaaagg cttgcaggca
     6361 agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt
     6421 gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac
     6481 atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc
     6541 accagctaac ataaaatgta agctctcggg gctctcttgc cttccaaccc agtcagaaat
     6601 cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa
     6661 cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag
     6721 tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg
     6781 cagttggacg atcgatgata agctgtcaaa catgagaatt gggtaataac tgatataatt
     6841 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt
     6901 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac
     6961 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag
     7021 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt
     7081 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca
     7141 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag
     7201 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca
     7261 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac
     7321 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac
     7381 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga
     7441 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa
     7501 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa
     7561 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct
     7621 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt
     7681 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcatgttttt gttctgtgca
     7741 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
     7801 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
     7861 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt
     7921 gaaaagctaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7981 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9301 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
