LOCUS       sequence-213842-       11641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG 
            Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Patterson K, Shavarebi F, Magnan C, Chang I, Qi X, Baldi P, 
            Bilanchone V, Sandmeyer SB
  TITLE     Local features determine Ty3 targeting frequency at RNA polymerase 
            III transcription start sites.
  JOURNAL   Genome Res. 2019 Aug;29(8):1298-1309. doi: 10.1101/gr.240861.118. 
            Epub 2019 Jun 27.
  PUBMED    31249062
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.240861.118"; journalName: "Genome Res"; date: "2019-08";
            volume: "29"; issue: "8"; pages: "1298-1309"
COMMENT     Sequence Label: pKP4010
FEATURES             Location/Qualifiers
     source          1..11641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      133..382
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(149..167)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(2028..2051)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2055..2078)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2082..2105)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2129..2152)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        2876..3219
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3220..4029
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3287..3306)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4656..4672
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4657..4673
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6192..6379
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6247..6271)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     misc_feature    7080..7372
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      7812..8265
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(8600..9403)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9404..9624)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9649..9667)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9735..9839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9840..9908,9909..10700)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10058..10077)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10871..11459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11360..11379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11613..11630
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacttaa ttaagtttaa actccggaca agatggcctt tggtgggttg aagaaggaaa
       61 aagacagaaa cgacttaatt acctacttga aaaaagcctg tgagtaaaca ggcccctttt
      121 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct cctcccacat
      181 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      241 tttaatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
      301 gtacaaacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
      361 acgctcgaag gctttaattt gcaaagtcag ggtgacgtat tgtcatactg acatatctca
      421 ttttgagata caacatcttc ctctctctca gggttgtctt gtgtttcgta gagttgtcta
      481 aagttggcta atattgatct tcgtgttctt tcgggaattt ggcaaaattc agcttctgag
      541 tattctactg aaagtgttgg gtctacatct tgcatgtgac ataagtaagt tttgtgtgta
      601 gtatctattc ctatgagtgc agtaacttcg tgtgctctct taattctttc agtggagctg
      661 attggtttat tctttgggta cgcgtctgga cggtatacaa actttttcag gaattgtaca
      721 ttaataactc tgtgcttttt cttgtgagag tttaaatcta gttcgtaggc gttatcgttt
      781 attttcttga caactcgaaa tggtccgacg tatatttgtt gtactttcat ataagcacct
      841 ttcttgaagt atgcatctct atgcactaat acgtgatctc ctatgtttaa caataagggt
      901 ttacgtcttt gattgttatt agtttccatt tcgatttgag cgtgttctag ctgttccttc
      961 gtttggatgg taagggcttt gaggtgtttg gcaagttcta cggcagtaaa acttcttgcg
     1021 ttgacttcgt catctgactt aatagcaggg gtattcggta aatatcctaa atcaatttca
     1081 aatggtgatt ttccaagtgt tctagtaggt gtagaattgt aaacaaattc gatttgtggt
     1141 aaatatacat gccaattctg aatattggtt gaagcatagg ctcttagtaa cctgtttaat
     1201 gtctgtatcg ttcgttcgga ttgtccatct gtttgggggt ggttcgcgga agacattgtc
     1261 gattttattc ctagtctttt cgtgagttct tgatatttgt cggcggtcat acggacatct
     1321 ctatcactgg ttattgtcct gggaaaacca tgatatgaaa aaatgtatcg aaagagtaga
     1381 tctattagtt gtgttgcgtc taaggttttc cttgtagcta tgaagtgagc gcgtttcgaa
     1441 aaacgatcaa ctacgacgag gatcatattc aagttatttg atgtcggggg taatcctgtc
     1501 acaaaatcca ttgatatatc aagccatctt ccttctgcta tagggagtgg ttgtaatagt
     1561 ccatgtaagc gtggtcggtg tgattttatt agttgacatt gtacgcaggt cctgatgtat
     1621 tgtatgatcg aatgttgtaa ttttggccaa tagtaaattg ggctgatttt cgcaagggtc
     1681 actgttacac caaaatgtcc tccaaataag gtatggtcat gatatagtct cataactgcg
     1741 ttctgttgtt ttattggtac tactagtcgg tcttggtaat agatcatttc gtcttctagg
     1801 gaataattct ttcggaaggt ctctgatagt tcgagtttct tctggtaact acggaaggct
     1861 gacatatctt caggtgtgac gttgtgttgt gtcaattctt tcatatgaat taagacagca
     1921 ctacataatg ggtctgattt gtagtaagat ttccagcttt ctgtgtcgat aggtcgggat
     1981 gtttcggggg ttatagtcat accgcctcca cccccgcctc cccctccctt atcgtcgtca
     2041 tccttgtaat cccccttatc gtcgtcatcc ttgtaatccc ccttatcgtc gtcatccttg
     2101 taatcaccgt atatagtttt ttctccttga cgttaaagta tagaggtata ttaacaattt
     2161 tttgttgata cttttatgac atttgaataa gaagtaatac aaactgaaaa tgttgaaagt
     2221 attagttaaa gtggttatgc agcttttcca tttatatatc tgttaataga tcaaaaatca
     2281 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
     2341 atggttgtta atttgattcg ttaatttgaa ggtttgtggg gccaggttac tgccaatttt
     2401 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
     2461 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggtctagac
     2521 tcctccgccg cactcctccg ccgctcctcc tccgccgcac tcctccgccg ctcctcctcc
     2581 gccgcactcc tccgccgcgg ccgcacttca atatagcaat gagcagttaa gcgtattact
     2641 gaaagttcca aagagaaggt ttttttaggc taagataatg gggctcttta catttccaca
     2701 acatataagt aagattagat atggatatgt atatggatat gtatatggtg gtaatgccat
     2761 gtaatatgat tattaaactt ctttgcgtcc atccaaaaaa aaagtaagaa tttttgaaaa
     2821 ttcaatataa agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     2881 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2941 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     3001 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     3061 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     3121 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     3181 aggatacagt tctcacatca catccgaaca taaacaacca tgggtaagga aaagactcac
     3241 gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3301 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3361 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3421 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3481 actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta
     3541 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3601 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3661 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3721 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     3781 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3841 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3901 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3961 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4021 tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     4081 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     4141 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gggccggccc gtacgctcac
     4201 cctaacatat tttccaatta accctcaata tttctctgtc acccggcctc tattttccat
     4261 tttcttcttt acccgccacg cgtttttttc tttcaaattt ttttcttcct tcttcttttt
     4321 cttccacgtc ctcttgcata aataaataaa ccgttttgaa accaaactcg cctctctctc
     4381 tcctttttga aatatttttg ggtttgtttg atcctttcct tcccaatctc tcttgtttaa
     4441 tatatattca tttatatcac gctctctttt tatcttcctt tttttcctct ctcttgtatt
     4501 cttccttccc ctttctactc aaaccaagaa gaaaaagaaa aggtcaatct ttgttaaaga
     4561 ataggatctt ctactacatc agcttttaga tttttcacgc ttactgcttt tttcttccca
     4621 agatcgaaaa tttactgaat taacagggcc ccccctcgag gtcgacggta tcgataagct
     4681 tgaagcaagc ctcctgaaag atgggtaccc gcccatatgc ttgccctgtc gagtcctgcg
     4741 atcgccgctt ttctcgccac gccaatctta cccgccatat ccgcatccat accggtcaga
     4801 agcccttcca gtgtcgaatc tgcatgcgta acttcagtcg taatgcgaac cttgtgcgcc
     4861 acatccgcac ccacacagga tcccaaaagc cgttccaatg tcggatctgt atgcggaact
     4921 ttagtcgaaa ggccgacctg aggcgtcaca ttcgcacgca caccggcgag aagccttttg
     4981 cctgtgacat ttgtgggagg aagtttgcca ggaagggcga cctcaagagg cataccaaaa
     5041 tccatacagg tggcggaggc acacctgcag ctgcgtcgac tctagaggat ccatctgctg
     5101 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
     5161 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     5221 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
     5281 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     5341 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
     5401 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
     5461 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     5521 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     5581 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     5641 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
     5701 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     5761 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
     5821 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggagatgc
     5881 tggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
     5941 accaaagcca cttggccact gcgggctcta cttcatcgga gctccactta gacggcgagg
     6001 acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg
     6061 gggattcccc gggtccggga tttacccccc acgactccgc cccctacggc gctctggata
     6121 tggccgactt cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg
     6181 ggtagtaata agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
     6241 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
     6301 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag atcgctctta
     6361 ttgaccacac ctctaccggc atgccgagca aatgcctgca aatcgctccc cctcagcatt
     6421 taaatcctgc agggaattcc tttctgcatg ccacgttcgc agcgcctgat gcggtatttt
     6481 ctccttacgc atctgtgcgg tatttcacac cgcataatcg ctgggccatt ctcatgaaga
     6541 atatcttgaa tttattgtca tattactagt tggtgtggaa gtcctaatat cggtgatcaa
     6601 tatagtggtt gacatgctgg ctagtcaaca ttgagccttt tgatcatgca aatatattac
     6661 ggtattttac aatcaaatat caaacttaac tattgacttt ataacttatt taggtggtaa
     6721 cattcttata aaaaagaaaa aaattactgc aaaacagtac tagcttttaa cttgtatcct
     6781 aggttatcta tgctgtctca ccatagagaa tattacctat ttcagaatgt atgtccatga
     6841 ttcgccgggt aaatacatat aatacacaaa tctggcttaa taaagtctat aatatatctc
     6901 ataaagaagt gctaaattgg ctagtgctat atatttttaa gaaaatttct tttgactaag
     6961 tccatatcga ctttgtaaaa gttcacatta gcatacatat attacacgag ccagaaatag
     7021 taacttttgc ctaaaatcac aaattgcaaa atttaattgc ttgcaaaagg tcacatgctt
     7081 ataatcaact tttttaaaaa tttaaaatac ttttttattt tttattttta aacataaatg
     7141 aaataattta tttattgttt atgattaccg aaacataaaa cctgctcaag aaaaagaaac
     7201 tgttttgtcc ttggaaaaaa agcactacct aggagcggcc aaaatgccga ggctttcata
     7261 gcttaaactc tttacagaaa ataggcatta tagatcagtt cgagttttct tattcttcct
     7321 tccggtttta tcgtcacagt tttacagtaa ataagtatca cctcttagag ttcgatgata
     7381 agctgtcaaa catgagaatt aattccacat gttaaaatag tgaaggagca tgttcggcac
     7441 acagtggacc gaacgtgggg taagatcgat gataagctgt caaacattgc actagggtcc
     7501 ggttaaacgg atctcgcatt gatgaggcaa cgctaattat caacatatag attgttatct
     7561 atctgcatga acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga
     7621 aaccactgtg tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt
     7681 aaaagcggtt aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg
     7741 ataaatacaa gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat
     7801 actaagctgc cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg
     7861 tagcgtatgc gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt
     7921 aaaagtcaac cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt
     7981 tagcattatc tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc
     8041 ttttcaaaag gcttgcaggc aagtgcacaa acaatactta aataaatact actcagtaat
     8101 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt
     8161 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc
     8221 aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctctcgg ggctctcttg
     8281 ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc
     8341 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt
     8401 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc
     8461 ttgccacgac tcatctccat gcagttggac gatcgatgat aagctgtcaa acatgagaat
     8521 tgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
     8581 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
     8641 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
     8701 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
     8761 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
     8821 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
     8881 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
     8941 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
     9001 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
     9061 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
     9121 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     9181 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     9241 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     9301 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     9361 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
     9421 tgcatgtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
     9481 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
     9541 gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa
     9601 aaaaaaatga tgaattgaat tgaaaagcta attcttgaag acgaaagggc ctcgtgatac
     9661 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     9721 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9781 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9841 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9901 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9961 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10021 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10081 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10141 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10201 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10261 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10321 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10381 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10441 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10501 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10561 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10621 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10681 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10741 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10801 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10861 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10921 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10981 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    11041 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11101 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11161 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11221 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11281 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11341 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11401 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11461 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11521 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11581 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11641 a
//
