LOCUS       sequence-213862-        6707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GABA Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, 
            Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
            Ahrens MB, Kullmann DM, Looger LL
  TITLE     A genetically encoded fluorescent sensor for in vivo imaging of 
            GABA.
  JOURNAL   Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
            Epub 2019 Jul 15.
  PUBMED    31308547
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-019-0471-2"; journalName: "Nat Methods"; date: 
            "2019-08"; volume: "16"; issue: "8"; pages: "763-770"
COMMENT     Sequence Label: pAAV.GFAP.iGABASnFR
FEATURES             Location/Qualifiers
     source          1..6707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(441..1029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(521..540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1200..1991,1992..2060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2061..2165)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2191..2646)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2328..2349)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2540..2559
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2774..2796
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2788..2805
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2789..2805
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2890..3019
                     /label=AAV2 ITR
     regulatory      3771..3780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3777..3836
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     4883..4904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5007..5028)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             5535..5564
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5568..5714
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    5730..6318
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5783..5803)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6201..6212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6321..6337)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6322..6338)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    6367..6488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6378..6397)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6432..6451
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
       61 acggttatcc acagaatcag gggataacgc aggaaanaga acatgtgagc aaaaggccag
      121 caaaaggcca ggaaccagmt aactcacatt aattgcgttg cgctcactgc cctagagcat
      181 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
      241 gagttggcca ctccctctct gcgcgctcgc tcgctcactg actcgctgcg ctcggtcgtt
      301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      841 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2161 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     2221 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     2281 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     2341 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     2401 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     2461 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     2521 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     2581 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     2641 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2701 cttcgctatt acgccagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg
     2761 ggggcagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaaca gggcgcgtcc
     2821 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     2881 ttacgccagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg
     2941 gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac
     3001 tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag
     3061 ccatgctcta ggaagatcca acatatcctg gtgtggagta ggggacgctg ctctgacaga
     3121 ggctcggggg cctgagctgg ctctgtgagc tggggaggag gcagacagcc aggcctttgt
     3181 ctgcaagcag acctggcagc attgggctgg ccgcccccca gggcctcctc ttcatgccca
     3241 gtgaatgact caccttggca cagacacaat gttcggggtg ggcacagtgc ctgcttcccg
     3301 ccgcacccca gcccccctca aatgccttcc gagaagccca ttgagcaggg ggcttgcatt
     3361 gcaccccagc ctgacagcct ggcatcttgg gataaaagca gcacagcccc ctaggggctg
     3421 cccttgctgt gtggcgccac cggcggtgga gaacaaggct ctattcagcc tgtgcccagg
     3481 aaaggggatc aggggatgcc caggcatgga cagtgggtgg caggggggga gaggagggct
     3541 gtctgcttcc cagaagtcca aggacacaaa tgggtgaggg gagagctctc cccatagctg
     3601 ggctgcggcc caaccccacc ccctcaggct atgccagggg gtgttgccag gggcacccgg
     3661 gcatcgccag tctagcccac tccttcataa agccctcgca tcccaggagc gagcagagcc
     3721 agagcaggtt ggagaggaga cgcatcacct ccgctgctcg cggggatccc gccaccatgg
     3781 agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca
     3841 gatctgaaag catcaatttc gtgagctggg gcggtagcac ccaggatgcg cagaagcagg
     3901 cctgggccga cccgttcagc aaggccagcg gcattaccgt ggtccaggat gggcccaccg
     3961 actacggcaa actcaaggcc atggtcgaaa gcggcaacgt gcagtgggac gtggtggatg
     4021 tagaggccga cttcgccttg cgcgccgccg ccgaaggcct gctcgaaccc ctggatttct
     4081 cggtgatcca gcgcgacaag atcgacccgc gcttcgtctc cgaccatggc gtgggctcgt
     4141 tcctgttctc tttcgtgctc ggctacaacg aaggcaagct cggagccagc aagccccagg
     4201 actggaccgc gctgttcgac accaagacct accccggcaa acgcgccctc tacaaatggc
     4261 cgagccctgg cgtgctcgaa ctggccctgc tcgccgacgg cgtaccggcc gacaagctct
     4321 acccgctgga cctggaccgc gccttcaaga aactcgacac catcaagaaa gacatcgtct
     4381 ggtggggcgg cggtgcacag tcgcagcagc tgctggcctc cggcgaagtc agcatgggcc
     4441 agttctggaa cggtcgcatc cacgccctgc aagaagacgg cgcgccagtg ggcgtgagct
     4501 ggaagcagaa cctggtgatg gccgacatcc tcgtcgtgcc taaaggcacg aagaacaaag
     4561 ccgccgcgat gaagttcctg gccagtgcca gcagcgccaa aggccaggac gacttttccg
     4621 ccctgaccgc ctatgctcca gtgaacatcg acagtgtgca gcgcctcgac cttgcgcagg
     4681 tccggatcac cgccgacaag cagaagaacg gcatcatggc gaacttcaag atccgccaca
     4741 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4801 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgtg ctgagcaaag
     4861 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4921 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatgagc aagggcgagg
     4981 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5041 agttcagcgt gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt
     5101 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     5161 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5221 ccgccatgcc cgaaggctac gtccaggagc gcaccatcag cttcaaggac gacggcacct
     5281 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5341 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactgga
     5401 acgccaacct ggcaccgaac ctgccgaccg cctacgtcaa ggatcagatc accctcgatt
     5461 tcgcctactg ggccaagaac ggtccggcca ttgcgacacg gtggaatgaa tggctagtca
     5521 aactgcaggt cgacgaacaa aaactcatct cagaagagga tctgaatgct gtgggccagg
     5581 acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg gtgatctcag
     5641 ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc atgctttggc
     5701 agaagaagcc acgttagaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     5761 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5821 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5881 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5941 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6001 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6061 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6121 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6181 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6241 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6301 tttgggccgc ctccccgcat cgataccgtc gacctcgacc cgggcggccg cttcgagcag
     6361 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     6421 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6481 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     6541 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta
     6601 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta
     6661 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactga
//
