LOCUS       sequence-213904-        3157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human alpha5 ectodomain with secretion peptide, purification
            tags and C-terminal clasp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Li J, Su Y, Xia W, Qin Y, Humphries MJ, Vestweber D, Cabanas C, Lu 
            C, Springer TA
  TITLE     Conformational equilibria and intrinsic affinities define integrin 
            activation.
  JOURNAL   EMBO J. 2017 Mar 1;36(5):629-645. doi: 10.15252/embj.201695803. Epub
            2017 Jan 25.
  PUBMED    28122868
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201695803"; journalName: "EMBO J"; date: "2017-03-1- 
            1"; volume: "36"; issue: "5"; pages: "629-645"
COMMENT     Sequence Label: a5-ecto-pcDNA31hygro
FEATURES             Location/Qualifiers
     source          1..3157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3007..3027
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3091..3102
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3127..3150
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 tctagagcta tggggagccg gacgccagag tcccctctcc acgccgtgca gctgcgctgg
       61 ggcccccggc gccgaccccc gctgctgccg ctgctgttgc tgctgctgcc gccgccaccc
      121 agggtcgggg gcttcaactt agacgcggag gccccagcag tactctcggg gcccccgggc
      181 tccttcttcg gattctcagt ggagttttac cggccgggaa cagacggggt cagtgtgctg
      241 gtgggagcac ccaaggctaa taccagccag ccaggagtgc tgcagggtgg tgctgtctac
      301 ctctgtcctt ggggtgccag ccccacacag tgcaccccca ttgaatttga cagcaaaggc
      361 tctcggctcc tggagtcctc actgtccagc tcagagggag aggagcctgt ggagtacaag
      421 tccttgcagt ggttcggggc aacagttcga gcccatggct cctccatctt ggcatgcgct
      481 ccactgtaca gctggcgcac agagaaggag ccactgagcg accccgtggg cacctgctac
      541 ctctccacag ataacttcac ccgaattctg gagtatgcac cctgccgctc agatttcagc
      601 tgggcagcag gacagggtta ctgccaagga ggcttcagtg ccgagttcac caagactggc
      661 cgtgtggttt taggtggacc aggaagctat ttctggcaag gccagatcct gtctgccact
      721 caggagcaga ttgcagaatc ttattacccc gagtacctga tcaacctggt tcaggggcag
      781 ctgcagactc gccaggccag ttccatctat gatgacagct acctaggata ctctgtggct
      841 gttggtgaat tcagtggtga tgacacagaa gactttgttg ctggtgtgcc caaagggaac
      901 ctcacttacg gctatgtcac catccttaat ggctcagaca ttcgatccct ctacaacttc
      961 tcaggggaac agatggcctc ctactttggc tatgcagtgg ccgccacaga cgtcaatggg
     1021 gacgggctgg atgacttgct ggtgggggca cccctgctca tggatcggac ccctgacggg
     1081 cggcctcagg aggtgggcag ggtctacgtc tacctgcagc acccagccgg catagagccc
     1141 acgcccaccc ttaccctcac tggccatgat gagtttggcc gatttggcag ctccttgacc
     1201 cccctggggg acctggacca ggatggctac aatgatgtgg ccatcggggc tccctttggt
     1261 ggggagaccc agcagggagt agtgtttgta tttcctgggg gcccaggagg gctgggctct
     1321 aagccttccc aggttctgca gcccctgtgg gcagccagcc acaccccaga cttctttggc
     1381 tctgcccttc gaggaggccg agacctggat ggcaatggat atcctgatct gattgtgggg
     1441 tcctttggtg tggacaaggc tgtggtatac aggggccgcc ccatcgtgtc cgctagtgcc
     1501 tccctcacca tcttccccgc catgttcaac ccagaggagc ggagctgcag cttagagggg
     1561 aaccctgtgg cctgcatcaa ccttagcttc tgcctcaatg cttctggaaa acacgttgct
     1621 gactccattg gtttcacagt ggaacttcag ctggactggc agaagcagaa gggaggggta
     1681 cggcgggcac tgttcctggc ctccaggcag gcaaccctga cccagaccct gctcatccag
     1741 aatggggctc gagaggattg cagagagatg aagatctacc tcaggaacga gtcagaattt
     1801 cgagacaaac tctcgccgat tcacatcgct ctcaacttct ccttggaccc ccaagcccca
     1861 gtggacagcc acggcctcag gccagcccta cattatcaga gcaagagccg gatagaggac
     1921 aaggctcaga tcttgctgga ctgtggagaa gacaacatct gtgtgcctga cctgcagctg
     1981 gaagtgtttg gggagcagaa ccatgtgtac ctgggtgaca agaatgccct gaacctcact
     2041 ttccatgccc agaatgtggg tgagggtggc gcctatgagg ctgagcttcg ggtcaccgcc
     2101 cctccagagg ctgagtactc aggactcgtc agacacccag ggaacttctc cagcctgagc
     2161 tgtgactact ttgccgtgaa ccagagccgc ctgctggtgt gtgacctggg caaccccatg
     2221 aaggcaggag ccagtctgtg gggtggcctt cggtttacag tccctcatct ccgggacact
     2281 aagaaaacca tccagtttga cttccagatc ctcagcaaga atctcaacaa ctcgcaaagc
     2341 gacgtggttt cctttcggct ctccgtggag gctcaggccc aggtcaccct gaacggtgtc
     2401 tccaagcctg aggcagtgct attcccagta agcgactggc atccccgaga ccagcctcag
     2461 aaggaggagg acctgggacc tgctgtccac catgtctatg agctcatcaa ccaaggcccc
     2521 agctccatta gccagggtgt gctggaactc agctgtcccc aggctctgga aggtcagcag
     2581 ctcctatatg tgaccagagt tacgggactc aactgcacca ccaatcaccc cattaaccca
     2641 aagggcctgg agttggatcc cgagggttcc ctgcaccacc agcaaaaacg ggaagctcca
     2701 agccgcagct ctgcttcctc gggacctcag atcctgaaat gcccggaggc tgagtgtttc
     2761 aggctgcgct gtgagctcgg gcccctgcac caacaagaga gccaaagtct gcagttgcat
     2821 ttccgagtct gggccaagac tttcttgcag cgggagcacc agccatttag cctgcagtgt
     2881 gaggctgtgt acaaagccct gaagatgccc taccgaatcc tgcctcggca gctgccccaa
     2941 aaagagcgtc aggtggccac agctgtgcaa tggaccaagg cagaaggcag ctataccggt
     3001 ggactggaga atctttattt ccaaggtgga gaaaatgctc agtgcgaaaa agagctccag
     3061 gcacttgaga aggaaaatgc acagctggaa tgggaattac aagcactaga gaaggagcta
     3121 gctcaatgga gccaccccca gttcgaaaag taagctt
//
