LOCUS       sequence-213908-       13518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  galactose-inducible Ty3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Hansen LJ, Chalker DL, Sandmeyer SB
  TITLE     Ty3, a yeast retrotransposon associated with tRNA genes, has 
            homology to animal retroviruses.
  JOURNAL   Mol Cell Biol. 1988 Dec;8(12):5245-56.
  PUBMED    2854194
REFERENCE   2  (bases 1 to 13518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1988-12"; volume: "8(12)"; pages: "5245-56"
COMMENT     Sequence Label: pDLC201
FEATURES             Location/Qualifiers
     source          1..13518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6517..7859
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7480..7527)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(8208..8227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8209..8227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8248..8264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8248..8264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8261..8283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8272..8288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8296..8302,8303..8320,8321..8326))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8341..8362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8479..8496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8650..9238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8730..8749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9409..10200,10201..10269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQDQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRGLCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10032..10051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10270..10374)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10442..10460
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10498..10520)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10620..10639
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      11053..11566
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     11350..11371
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12105..12127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12119..12136
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12120..12136
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12146..12366
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             12367..13170
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     protein_bind    join(13427..13518,1..26)
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 tcacccgctc ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt
       61 actgaaagtt ccaaagagaa ggttttttta ggctaactcg agctcgactc tagaggatcc
      121 ccctgaacta cccaaagtat aaatgcctga acaattagtt tagatccgag attccgcgct
      181 tccaccactt agtatgattc atattttata taatatataa gataagtaac attccgtgaa
      241 ttaatctgat aaactgtttt gacaactggt tacttcccta agactgttta tattaggatt
      301 gtcaagacac tccggtatta ctcgagcccg taatacaaca cctggtagcg ttaaaggtta
      361 ctaattgttc aaacgaacca tcgaaaagcc gaacctagct acaccacacc ccagtatgag
      421 ctttatggat caaatcccag gaggaggaaa ttatccaaaa ctcccagtag aatgccttcc
      481 taacttcccg atccaaccat ctttgacctt cagaggtaga aatgactcgc ataaactgaa
      541 aaactttatc tccgaaataa tgttaaacat gtctatgata tcttggccga atgatgccag
      601 tcgtattgtg tactgcagaa gacatttatt aaaccccgct gctcagtggg ctaatgactt
      661 tgtacaagaa caaggtatac ttgaaataac attcgacaca ttcatacaag gattatatca
      721 gcatttctat aagccaccag atatcaataa aatctttaat gcaatcacgc aactttccga
      781 agctaaactt ggtattgagc gtctcaacca acgattcaga aagatttggg acagaatgcc
      841 accagacttc atgaccgaaa aagctgccat aatgacatat actaggctat tgacaaagga
      901 aacctataat attgtcagaa tgcacaaacc agagacatta aaagacgcca tggaagaggc
      961 ttaccagaca actgcactaa ctgaaagatt cttcccagga ttcgaacttg atgctgatgg
     1021 agacactatc atcggtgcca caacccactt acaagaagaa tacgactctg actatgattc
     1081 agaagataat ctgacccaga atggatacgt ccataccgta aggacaagaa gatcttacaa
     1141 taaaccaatg tcaaatcatc gaaacaggag aaataacaac ccatctagag aagaatgtat
     1201 aaaaaatcgg ctatgcttct attgtaagaa agagggacat cgcctgaacg aatgtagagc
     1261 acgtaaggcg agttctaacc gatcttgaac tcgaatcaaa agaccaacaa actcctttta
     1321 tcaaaacctt accaattgta cactatatcg ccatccccga gatggacaat accgccgaaa
     1381 aaaccataaa aatacaaaac acgaaagtaa aaaccctgtt tgacagtgga tcacccacgt
     1441 catttatccg aagagatatt gtagaacttc tcaaatacga aatctacgag acccctccac
     1501 tccgttttag aggattcgta gccaccaaat ccgccgttac atccgaagca gtcaccattg
     1561 acctcaaaat caatgacctg catataactt tagccgcgta catactggat aacatggact
     1621 accaattgtt aattggaaat ccaatcttac gccgctaccc gaaaatcctg cacacagtac
     1681 tgaataccag agagagcccc gactccttaa agcccaagac ttatcgctcc gaaaccgtta
     1741 ataacgttag aacctactcc gctggtaatc gtggtaaccc cagaaacata aaactgtctt
     1801 ttgcccccac cattctcgaa gcaactgacc cgaaatccgc tggtaatcgt ggtgactcca
     1861 gaaccaaaac cctgtctctt gcaaccacta ctcctgcagc aattgacccg cttacgaccc
     1921 ttgataaccc aggtagtact caaagtacat ttgcgcaatt cccgatacct gaagaagcga
     1981 gcatcctaga agaggatgga aaatactcca acgttgtctc aaccattcag agtgtagaac
     2041 ctaatgctac tgatcacagc aataaggaca ccttttgcac tttgccagtt tggttacaac
     2101 agaagtatag agagatcata cgtaatgatc tcccaccaag acctgccgac attaataaca
     2161 tccccgtaaa acatgatatt gaaattaaac ctggcgcaag actacctcga ctacagccat
     2221 accatgttac agaaaagaac gaacaagaaa tcaacaaaat agttcaaaaa ctgctcgata
     2281 acaagttcat tgttccctca aagtcgcctt gcagctcccc tgtagtcctc gtcccgaaga
     2341 aagacggtac cttccgactc tgcgtcgatt accgcaccct gaacaaagct accatctccg
     2401 acccattccc attacccaga atcgacaacc tattgagccg tattggaaat gcccagatat
     2461 ttaccacgct agatttgcat agtggttacc accagatccc gatggaaccc aaagaccgct
     2521 acaaaaccgc ctttgtcaca ccatccggta agtatgaata taccgtcatg ccatttggct
     2581 tagtcaatgc acctagtaca ttcgcaagat acatggctga tacatttaga gacctgagat
     2641 tcgtcaatgt ttaccttgat gatatattaa tattctccga atctccagaa gaacattgga
     2701 aacatttaga cacggtacta gaaagattaa agaacgagaa cctcattgtt aagaagaaaa
     2761 aatgtaaatt tgcatctgaa gaaactgagt ttttaggcta tagtattgga atccagaaaa
     2821 tagctccact acagcacaaa tgtgcagcaa tccgagactt tccgacgcct aaaacagtaa
     2881 aacaagcaca gagattttta ggaatgatta attactacag acgattcatt ccaaattgct
     2941 ccaagattgc acagccaatc caactgttta tttgtgacaa aagtcaatgg acagaaaaac
     3001 aagacaaggc aattgataaa ctaaaagacg ccttgtgtaa ctcccccgtc ctagtaccat
     3061 tcaacaacaa agcaaactac cgacttacaa cagacgcctc aaaagacggc attggtgctg
     3121 ttctagaaga agtcgacaac aagaacaaac ttgttggtgt cgtcggttac ttctctaaat
     3181 ccttagagag tgcccagaaa aactatcctg ctggcgaatt agaactactt ggaattatca
     3241 aagcactcca ccacttccga tatatgcttc acggaaagca tttcacgtta agaacagacc
     3301 acattagttt gttatcatta caaaacaaga acgaacccga cgaacgcgtg caacgctggt
     3361 tagatgacct agccacatat gacttcacct tagaatacct agctggaccc aagaacgttg
     3421 tcgcagatgc catatcccgt gccgtatata ctataacccc cgaaacatcc cgacctatcg
     3481 acacagaaag ctggaaatct tactacaaat cagacccatt atgtagtgct gtcttaattc
     3541 atatgaaaga attgacacaa cacaacgtca cacctgaaga tatgtcagcc ttccgtagtt
     3601 accagaagaa actcgaacta tcagagacct tccgaaagaa ttattcccta gaagacgaaa
     3661 tgatctatta ccaagaccga ctagtagtac caataaaaca acagaacgca gttatgagac
     3721 tatatcatga ccatacctta tttggaggac attttggtgt aacagtgacc cttgcgaaaa
     3781 tcagcccaat ttactattgg ccaaaattac aacattcgat catacaatac atcaggacct
     3841 gcgtacaatg tcaactaata aaatcacacc gaccacgctt acatggacta ttacaaccac
     3901 tccctatagc agaaggaaga tggcttgata tatcaatgga ttttgtgaca ggattacccc
     3961 cgacatcaaa taacttgaat atgatcctcg tcgtagttga tcgtttttcg aaacgcgctc
     4021 acttcatagc tacaaggaaa accttagacg caacacaact aatagatcta ctctttcgat
     4081 acattttttc atatcatggt tttcccagga caataaccag tgatagagat gtccgtatga
     4141 ccgccgacaa atatcaagaa ctcacgaaaa gactaggaat aaaatcgaca atgtcttccg
     4201 cgaaccaccc ccaaacagat ggacaatccg aacgaacgat acagacatta aacaggttac
     4261 taagagccta tgcttcaacc aatattcaga attggcatgt atatttacca caaatcgaat
     4321 ttgtttacaa ttctacacct actagaacac ttggaaaatc accatttgaa attgatttag
     4381 gatatttacc gaatacccct gctattaagt cagatgacga agtcaacgca agaagtttta
     4441 ctgccgtaga acttgccaaa cacctcaaag cccttaccat ccaaacgaag gaacagctag
     4501 aacacgctca aatcgaaatg gaaactaata acaatcaaag acgtaaaccc ttattgttaa
     4561 acataggaga tcacgtatta gtgcatagag atgcatactt caagaaaggt gcttatatga
     4621 aagtacaaca aatatacgtc ggaccatttc gagttgtcaa gaaaataaac gataacgcct
     4681 acgaactaga tttaaactct cacaagaaaa agcacagagt tattaatgta caattcctga
     4741 aaaagtttgt ataccgtcca gacgcgtacc caaagaataa accaatcagc tccactgaaa
     4801 gaattaagag agcacacgaa gttactgcac tcataggaat agatactaca cacaaaactt
     4861 acttatgtca catgcaagat gtagacccaa cactttcagt agaatactca gaagctgaat
     4921 tttgccaaat tcccgaaaga acacgaagat caatattagc caactttaga caactctacg
     4981 aaacacaaga caaccctgag agagaggaag atgttgtatc tcaaaatgag atatgtcagt
     5041 atgacaatac gtcaccctga acgttcataa aacacatatg aaacaacctt ataacaaaac
     5101 gaacaacatg agacaaaacc cgaccttccc tagctgaact acccaaagta taaatgcctg
     5161 aacaattagt ttagatccga gattccgcgc ttccaccact tagtatgatt catattttat
     5221 ataatatata agataagtaa cattccgtga attaatctga taaactgttt tgactggttt
     5281 ccctaagact gtttatatta ggattgtcaa gacactccgg tattactcga gcccgtaata
     5341 caacagaaag ttccattttg gatgctctat ttatgggaat atgactatac gaatgtcacc
     5401 cacgatggcc ggagtagctg tgatgagcat ctttttgtct gggcggctta aaacgtgagc
     5461 aaaattgtac tgtaaattac ttacgttatc aatggaagtg tgtgaatttc gtgataaaat
     5521 tattgtcaga atagtgactt cattattctt tataactaac cattaggatc atataagtaa
     5581 tcagatgcag ttcgtcatta atatgtcagg atggataacc ataggtaagc acgtgattat
     5641 ggaaggacag taaagttacc gatctacttt tttgaatgat caacttggtg ttttcatcta
     5701 taaggatatg ggtcgttaca ttgaaactat agtaaaagat ctcgttattg gtatatcggt
     5761 cataatgaag atgaaatagt cggtatgtat ttttataccc ccccctatat ggtaaaaagg
     5821 ttaagattca tatttaaatc ttcattaata ccacctctaa tttctaatta ccaacatttt
     5881 taactccact tttattatat gctatataca acattatgta aaaggagtat accaaaaatt
     5941 ctccttcata atataggaat tccacggact atagactata ctagtatact ccgtctactg
     6001 tacgatacac ttccgctcag gtccttgtcc tttaacgagg ccttaccact cttttgttac
     6061 tctattgatc cagctcagca aaggcagtgt gatctaagat tctatcttcg cgatgtagta
     6121 aaactagcta gaccgagaaa gagactagaa atgcaaaagg cacttctaca atggctgcca
     6181 tcattattat ccgatgtgac gctgcagctt ctcaatgata ttcgaatacg ctttgaggag
     6241 atacagccta atatccgaca aactgtttta cagatttacg atcgtacttg ttacccatca
     6301 ttgaattttg aacatccgaa cctgggagtt ttccctgaaa cagatagtat atttgaacct
     6361 gtataataat atatagtcta gcgctttacg gaagacaatg tatgtatttc ggttcctgga
     6421 gaaactattg catctattgc ataggtaatc ttgcacgtcg catccccggt tcattttctg
     6481 cgtttccatc ttgcacttca atagcatatc tttgttaacg aagcatctgt gcttcatttt
     6541 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     6601 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     6661 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     6721 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     6781 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     6841 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     6901 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     6961 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     7021 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     7081 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     7141 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     7201 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     7261 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     7321 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     7381 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     7441 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
     7501 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
     7561 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
     7621 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
     7681 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
     7741 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
     7801 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
     7861 tatgcatagt accgagaaac tagtgcgaag tagtgatcag gtattgctgt tatctgatga
     7921 gtatacgttg tcctggccac ggcagaagca cgcttatcgc tccaatttcc cacaacatta
     7981 gtcaactccg ttaggccctt cattgaaaga aatgaggtca tcaaatgtct tccaatgtga
     8041 gattttgggc cattttttat agcaaagatt gaataaggcg catttttctt caaagcttta
     8101 ttgtacgatc tgactaagtt atcttttaat aattggtatt cctgtttatt gcttgaagaa
     8161 ttgccggtcc tatttactcg ttttaggact ggttcagaat tcggtctccc tatagtgagt
     8221 cgtattaatt cgatatccgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8281 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8341 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8401 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8461 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8641 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9061 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9301 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9361 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9421 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9481 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9541 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9601 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9661 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9721 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9781 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9841 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9901 agcactgcat aatcctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9961 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttggt cttgcccggc
    10021 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10081 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg tttagatcca gttcgatgta
    10141 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10201 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10261 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10321 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10381 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10441 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10501 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10561 acaagcccgt cagggtgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10621 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10681 cgtaaggaga aaataccgca tcaggaantt ccagacgatt gagcgtcaaa atgtaggtat
    10741 ttccatgagc gtttttcctg ttgcaatggc tggcggtaat attgttctgg atattaccag
    10801 caaggccgat agtttgagtt cttctactca ggcaagtgat gttattacta atcaaagaag
    10861 tattgcgaca acggttaatt tgcgtgatgg acagactctt ttactcggtg gcctcactga
    10921 ttataaaaac acttctcagg attctggcgt accgttcctg tctaaaatcc ctttaatcgg
    10981 cctcctgttt agctcccgct ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa
    11041 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    11101 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttcccct
    11161 cctttgtcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    11221 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    11281 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    11341 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    11401 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    11461 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa atatttgctt
    11521 atacaatctt cctgtttttg gggcttttct gattatcaac cggggtacat atgattgaca
    11581 tgctagtttt acgattaccg ttcatcgatt ctcttgtttg ctccagactc tcaggcaatg
    11641 acctgatagc ctttgtagac ctctcaaaaa tagctaccct ctccggcatg aatttatcag
    11701 ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt tctcacccgt
    11761 ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag ggttctaaaa
    11821 atttttatcc ttgcgttgaa ataaaggatt ctcccgcaaa agtattacag ggtcataatg
    11881 tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat tttgctaatt
    11941 ctttgccttg cctgtatgat ttattggatg ttggaanttc cattcgccct tcaggctgcg
    12001 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    12061 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    12121 taaaacgacg gccagtgcca agcttttcaa ttcaattcat catttttttt ttattctttt
    12181 ttttgatttc ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa
    12241 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca
    12301 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga
    12361 taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct
    12421 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt
    12481 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta
    12541 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
    12601 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
    12661 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
    12721 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa
    12781 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
    12841 ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt
    12901 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg
    12961 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga
    13021 accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt
    13081 gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca
    13141 tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata
    13201 ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccga tcaaaaatca
    13261 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
    13321 atggttgtta atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt
    13381 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
    13441 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag
    13501 tcttccttcg gagggctg
//
