LOCUS       sequence-213934-         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BCCP-PupE in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Liu Q, Zheng J, Sun W, Huo Y, Zhang L, Hao P, Wang H, Zhuang M
  TITLE     A proximity-tagging system to identify membrane protein-protein 
            interactions.
  JOURNAL   Nat Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5.
  PUBMED    30104635
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5."
COMMENT     Sequence Label: pEF6a-BCCP-PupE
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             253..276
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gccgggaagg caggcgaggg agagatcccc gcacccttgg ccggcacggt cagcaaaatc
       61 ctggtcaagg aaggcgacac cgtgaaggct ggacagacgg tgttggtact ggaggcgatg
      121 aagatggaga cagagatcaa tgccccgacc gatgggaagg tggagaaggt gttggttaag
      181 gagagggacg ccgtgcaggg cggtcaggga ctgatcaaga tcggcgacta cgacatcccg
      241 acaaccgcca gcctggaagt tctgttccag gggcccggat ccatgaacgc gaaacagacc
      301 cagatcatgg gtggcggtgg tcgtgacgaa gacaatgcgg aagactctgc tcaggcgtct
      361 ggtcaggttc agatcaatac cgaaggtgtt gactctctgc tggacgaaat cgacggcctg
      421 ctcgaaaaca acgcggagga attcgttcgt tcttacgttc agaaaggtgg tgaa
//
