LOCUS       sequence-213935-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BCCP-PupE in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Liu Q, Zheng J, Sun W, Huo Y, Zhang L, Hao P, Wang H, Zhuang M
  TITLE     A proximity-tagging system to identify membrane protein-protein 
            interactions.
  JOURNAL   Nat Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5.
  PUBMED    30104635
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5."
COMMENT     Sequence Label: pEF6a-BCCP-PupE
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             295..318
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgaggggca gccaccacca tcatcaccac agccaggatc cggccgggaa ggcaggcgag
       61 ggagagatcc ccgcaccctt ggccggcacg gtcagcaaaa tcctggtcaa ggaaggcgac
      121 accgtgaagg ctggacagac ggtgttggta ctggaggcga tgaagatgga gacagagatc
      181 aatgccccga ccgatgggaa ggtggagaag gtgttggtta aggagaggga cgccgtgcag
      241 ggcggtcagg gactgatcaa gatcggcgac tacgacatcc cgacaaccgc cagcctggaa
      301 gttctgttcc aggggcccgg atccatgaac gcgaaacaga cccagatcat gggtggcggt
      361 ggtcgtgacg aagacaatgc ggaagactct gctcaggcgt ctggtcaggt tcagatcaat
      421 accgaaggtg ttgactctct gctggacgaa atcgacggcc tgctcgaaaa caacgcggag
      481 gaattcgttc gttcttacgt tcagaaaggt ggtgaa
//
