LOCUS       sequence-213936-        2221 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CD28-PafA in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Liu Q, Zheng J, Sun W, Huo Y, Zhang L, Hao P, Wang H, Zhuang M
  TITLE     A proximity-tagging system to identify membrane protein-protein 
            interactions.
  JOURNAL   Nat Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5.
  PUBMED    30104635
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5."
COMMENT     Sequence Label: pEF6a-CD28-PafA
FEATURES             Location/Qualifiers
     source          1..2221
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2180..2209
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgctcaggc tgctcttggc tctcaactta ttcccttcaa ttcaagtaac aggaaacaag
       61 attttggtga agcagtcgcc catgcttgta gcgtacgaca atgcggtcaa ccttagctgc
      121 aagtattcct acaatctctt ctcaagggag ttccgggcat cccttcacaa aggactggat
      181 agtgctgtgg aagtctgtgt tgtatatggg aattactccc agcagcttca ggtttactca
      241 aaaacggggt tcaactgtga tgggaaattg ggcaatgaat cagtgacatt ctacctccag
      301 aatttgtatg ttaaccaaac agatatttac ttctgcaaaa ttgaagttat gtatcctcct
      361 ccttacctag acaatgagaa gagcaatgga accattatcc atgtgaaagg gaaacacctt
      421 tgtccaagtc ccctatttcc cggaccttct aagccctttt gggtgctggt ggtggttggt
      481 ggagtcctgg cttgctatag cttgctagta acagtggcct ttattatttt ctgggtgagg
      541 agtaagagga gcaggctcct gcacagtgac tacatgaaca tgactccccg ccgccccggg
      601 cccacccgca agcattacca gccctatgcc ccaccacgcg acttcgcagc ctatcgctcc
      661 tctagagggc ccttcggctc cggtggcggc agcggtgggg ggcagtggag gcggaagcgg
      721 agggggctct ggtatgagta ccgtggaatc cgcattgacc cgcaggatca tgggcattga
      781 aacggagtat ggcctcacct ttgttgatgg tgattccaaa aagcttcgcc cagatgagat
      841 agctcgaagg atgtttcgtc ccatcgtgga gaaatattcc agctctaata tcttcatacc
      901 caatggttcc cggttgtatc ttgatgtggg ttcccacccg gagtacgcca ccgccgagtg
      961 tgataatttg acccagctga tcaattttga aaaagctggc gatgttattg cagatcgcat
     1021 ggctgtagat gccgaagagt cgctggcgaa agaagacatt gctgggcagg tgtacctgtt
     1081 taaaaacaat gtcgattccg tgggcaattc ttatggctgc cacgaaaact accttgtggg
     1141 tcgctccatg ccgttgaagg cgttgggtaa aaggctgatg ccgtttctga ttacccgcca
     1201 gctcatctgc ggcgccggca ggatccatca ccccaatcct ttggataaag gcgaatcctt
     1261 ccccttgggc tactgcatat cccagcgctc tgaccacgtg tgggagggcg tatcaagtgc
     1321 caccactaga tcacgcccca ttatcaacac ccgtgatgag ccacatgcgg attcccattc
     1381 ttaccgcagg ctgcacgtga ttgtgggtga tgccaacatg gcagagccca gcatcgcgtt
     1441 gaaggtcggc tccacgttgc tggttctgga aatgattgag gcagatttcg gtttgcccag
     1501 cttagagctt gccaatgata ttgcctcaat tagggaaatc tcccgcgatg caacaggatc
     1561 cacactgttg tccctgaaag atggcaccac catgactgcc ttgcagatcc agcaggtggt
     1621 ctttgagcat gcctcgaagt ggttggagca gcgccccgaa ccagaatttt ctggcacctc
     1681 caacacagag atggcccgcg tgctggatct gtggggtcgc atgttgaaag cgattgagtc
     1741 cggtgatttc agcgaagtgg atacagaaat tgactgggtg atcaaaaaga agctcattga
     1801 tcgtttcatt cagcgcggca accttgggtt ggatgatcca aaacttgccc aagtggactt
     1861 gacttatcac gatattaggc caggtagagg cctatttagc gtgctgcaaa gccgcggcat
     1921 gatcaaacgg tggactactg atgaggcgat tttagctgcg gtggataccg ctcctgatac
     1981 aacacgtgct catttgcgcg ggcgaatcct taaagcggcg gatactctgg gagtacctgt
     2041 gactgtcgat tggatgcgtc acaaggtcaa ccgaccggag ccacaatcgg tggaattggg
     2101 ggatcctttt tccgcagtga attccgaggt tgatcagttg attgagtata tgaccgttca
     2161 tgcggagagc tatcgcagtg aacaaaaact catctcagaa gaggatctga atatgcagta
     2221 a
//
