LOCUS       sequence-213947-        1785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FRB-PafA in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Liu Q, Zheng J, Sun W, Huo Y, Zhang L, Hao P, Wang H, Zhuang M
  TITLE     A proximity-tagging system to identify membrane protein-protein 
            interactions.
  JOURNAL   Nat Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5.
  PUBMED    30104635
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5."
COMMENT     Sequence Label: pGEX6p-1-FRB-PafA
FEATURES             Location/Qualifiers
     source          1..1785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..285
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
ORIGIN
        1 ccgattctgt ggcatgagat gtggcacgag ggactcgaag aggccagcag gctgtacttt
       61 ggcgagagga acgtcaaggg catgttcgaa gtgctggagc ccctccatgc gatgatggaa
      121 aggggcccac agaccctgaa ggagaccagc ttcaaccagg cttacggcag ggacctgatg
      181 gaggcacagg aatggtgcag gaagtacatg aagagcggca acgtgaaaga cctgacccag
      241 gcgtgggacc tctactacca cgtgttcagg aggatcagca agcagtccgg tggcggcagc
      301 ggtgggggca gtggaggcgg aagcggaggg ggctctggca tgagtaccgt ggaatccgca
      361 ttgacccgca ggatcatggg cattgaaacg gagtatggcc tcacctttgt tgatggtgat
      421 tccaaaaagc ttcgcccaga tgagatagct cgaaggatgt ttcgtcccat cgtggagaaa
      481 tattccagct ctaatatctt catacccaat ggttcccggt tgtatcttga tgtgggttcc
      541 cacccggagt acgccaccgc cgagtgtgat aatttgaccc agctgatcaa ttttgaaaaa
      601 gctggcgatg ttattgcaga tcgcatggct gtagatgccg aagagtcgct ggcgaaagaa
      661 gacattgctg ggcaggtgta cctgtttaaa aacaatgtcg attccgtggg caattcttat
      721 ggctgccacg aaaactacct tgtgggtcgc tccatgccgt tgaaggcgtt gggtaaaagg
      781 ctgatgccgt ttctgattac ccgccagctc atctgcggcg ccggcagaat ccatcacccc
      841 aatcctttgg ataaaggcga atccttcccc ttgggctact gcatatccca gcgctctgac
      901 cacgtgtggg agggcgtatc aagtgccacc actagatcac gccccattat caacacccgt
      961 gatgagccac atgcggattc ccattcttac cgcaggctgc acgtgattgt gggtgatgcc
     1021 aacatggcag agcccagcat cgcgttgaag gtcggctcca cgttgctggt tctggaaatg
     1081 attgaggcag atttcggttt gcccagctta gagcttgcca atgatattgc ctcaattagg
     1141 gaaatctccc gcgatgcaac aggttccaca ctgttgtccc tgaaagatgg caccaccatg
     1201 actgccttgc agatccagca ggtggtcttt gagcatgcct cgaagtggtt ggagcagcgc
     1261 cccgaaccag aattttctgg cacctccaac acagagatgg cccgcgtgct ggatctgtgg
     1321 ggtcgcatgt tgaaagcgat tgagtccggt gatttcagcg aagtggatac agaaattgac
     1381 tgggtgatca aaaagaagct cattgatcgt ttcattcagc gcggcaacct tgggttggat
     1441 gatccaaaac ttgcccaagt ggacttgact tatcacgata ttaggccagg tagaggccta
     1501 tttagcgtgc tgcaaagccg cggcatgatc aaacggtgga ctactgatga ggcgatttta
     1561 gctgcggtgg ataccgctcc tgatacaaca cgtgctcatt tgcgcgggcg aatccttaaa
     1621 gcggcggata ctctgggagt acctgtgact gtcgattgga tgcgtcacaa ggtcaaccga
     1681 ccggagccac aatcggtgga attgggagat cctttttccg cagtgaattc cgaggttgat
     1741 cagttgattg agtatatgac cgttcatgcg gagagctatc gcagt
//
