LOCUS       sequence-213949-        1714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express inducible BCCP-PupE in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Liu Q, Zheng J, Sun W, Huo Y, Zhang L, Hao P, Wang H, Zhuang M
  TITLE     A proximity-tagging system to identify membrane protein-protein 
            interactions.
  JOURNAL   Nat Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5.
  PUBMED    30104635
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Aug 13. pii: 10.1038/s41592-018-0100-5. doi: 
            10.1038/s41592-018-0100-5."
COMMENT     Sequence Label: pLVX-TRE3G-BCCP-PupE-IRES-BFP
FEATURES             Location/Qualifiers
     source          1..1714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    464..1013
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(608..625)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     835..854
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1016..1714
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atggccggga aggcaggcga gggagagatc cccgcaccct tggccggcac ggtcagcaaa
       61 atcctggtca aggaaggcga caccgtgaag gctggacaga cggtgttggt actggaggcg
      121 atgaagatgg agacagagat caatgccccg accgatggga aggtggagaa ggtgttggtt
      181 aaggaaaggg acgccgtgca gggcggtcag ggactgatca agatcggcga ctacgacatc
      241 ccgacaaccg ccagcatgaa cgcgaaacag acccagatca tgggtggcgg tggtcgtgac
      301 gaagacaatg cggaagactc tgctcaggcg tctggtcagg ttcagatcaa taccgaaggt
      361 gttgactctc tgctggacga aatcgacggc ctgctcgaaa acaacgcgga ggaattcgtt
      421 cgttcttacg ttcagaaagg tggtgaataa gccccccccc ctaacgttac tggccgaagc
      481 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
      541 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      601 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      661 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      721 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
      781 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
      841 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
      901 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
      961 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgag
     1021 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
     1081 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     1141 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
     1201 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
     1261 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     1321 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     1381 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     1441 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
     1501 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
     1561 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
     1621 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
     1681 cgacctccct agcaaactgg ggcacaagct taat
//
