LOCUS       sequence-213964-        9003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hA3D-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-hA3D-BE3
FEATURES             Location/Qualifiers
     source          1..9003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1615..5013,5014..5016,5017..5613,5614,5615..5617,
                     5618..5619,5620..5622,5623..5715)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1615..1638,1639..1641,1642..5715)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5728..5976
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5989..6009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6018..6035
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6058..6075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6064..6288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6359..6375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6359..6375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6372..6394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6383..6399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6407..6413,6414..6431,6432..6437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6452..6473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6590..6607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6761..7349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6841..6860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7520..8311,8312..8380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8143..8162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8381..8485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8564..8583)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8755..9003,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaatccacag
      421 atcagaaatc cgatggagcg gatgtatcga gacacattct acgacaactt tgaaaacgaa
      481 cccatcctct atggtcggag ctacacttgg ctgtgctatg aagtgaaaat aaagaggggc
      541 cgctcaaatc tcctttggga cacaggggtc tttcgaggcc cggtactacc caaacgtcag
      601 tcgaatcaca ggcaggaggt gtatttccgg tttgagaacc acgcagaaat gtgcttctta
      661 tcttggttct gtggcaaccg actgcctgct aacaggcgct tccagatcac ctggtttgta
      721 tcatggaacc cctgcctgcc ctgtgtggtg aaggtgacca aattcttggc tgagcacccc
      781 aatgtcaccc tgaccatctc tgccgcccgc ctctactact accgggatag agattggcgg
      841 tgggtgctcc tcaggctgca taaggcaggg gcccgtgtga agatcatgga ctatgaagac
      901 tttgcatact gctgggaaaa ctttgtgtgc aatgaaggtc agccattcat gccttggtac
      961 aaattcgatg acaattatgc atccctgcac cgcacgctaa aggagattct cagaaacccg
     1021 atggaggcaa tgtacccaca catattctac ttccacttta aaaacctact gaaagcctgt
     1081 ggtcggaacg aaagctggct gtgcttcacc atggaagtta caaagcacca ctcagctgtc
     1141 ttccggaaga ggggcgtctt ccgaaaccag gtggatcctg agacccattg tcatgcagaa
     1201 aggtgcttcc tctcttggtt ctgtgacgac atactgtctc ctaacacaaa ctacgaggtc
     1261 acctggtaca catcttggag cccttgccca gagtgtgcag gggaggtggc cgagttcctg
     1321 gccaggcaca gcaacgtgaa tctcaccatc ttcaccgccc gcctctgcta cttctgggat
     1381 acagattacc aggaggggct ctgcagcctg agtcaggaag gggcctccgt gaagatcatg
     1441 ggctacaaag attttgtatc ttgttggaaa aactttgtgt acagtgatga tgagccattc
     1501 aagccttgga agggactaca aaccaacttt cgacttctga aaagaaggct acgggagatt
     1561 ctccagagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagtgataaa
     1621 aagtattcta ttggtttagc catcggcact aattccgttg gatgggctgt cataaccgat
     1681 gaatacaaag taccttcaaa gaaatttaag gtgttgggga acacagaccg tcattcgatt
     1741 aaaaagaatc ttatcggtgc cctcctattc gatagtggcg aaacggcaga ggcgactcgc
     1801 ctgaaacgaa ccgctcggag aaggtataca cgtcgcaaga accgaatatg ttacttacaa
     1861 gaaattttta gcaatgagat ggccaaagtt gacgattctt tctttcaccg tttggaagag
     1921 tccttccttg tcgaagagga caagaaacat gaacggcacc ccatctttgg aaacatagta
     1981 gatgaggtgg catatcatga aaagtaccca acgatttatc acctcagaaa aaagctagtt
     2041 gactcaactg ataaagcgga cctgaggtta atctacttgg ctcttgccca tatgataaag
     2101 ttccgtgggc actttctcat tgagggtgat ctaaatccgg acaactcgga tgtcgacaaa
     2161 ctgttcatcc agttagtaca aacctataat cagttgtttg aagagaaccc tataaatgca
     2221 agtggcgtgg atgcgaaggc tattcttagc gcccgcctct ctaaatcccg acggctagaa
     2281 aacctgatcg cacaattacc cggagagaag aaaaatgggt tgttcggtaa ccttatagcg
     2341 ctctcactag gcctgacacc aaattttaag tcgaacttcg acttagctga agatgccaaa
     2401 ttgcagctta gtaaggacac gtacgatgac gatctcgaca atctactggc acaaattgga
     2461 gatcagtatg cggacttatt tttggctgcc aaaaacctta gcgatgcaat cctcctatct
     2521 gacatactga gagttaatac tgagattacc aaggcgccgt tatccgcttc aatgatcaaa
     2581 aggtacgatg aacatcacca agacttgaca cttctcaagg ccctagtccg tcagcaactg
     2641 cctgagaaat ataaggaaat attctttgat cagtcgaaaa acgggtacgc aggttatatt
     2701 gacggcggag cgagtcaaga ggaattctac aagtttatca aacccatatt agagaagatg
     2761 gatgggacgg aagagttgct tgtaaaactc aatcgcgaag atctactgcg aaagcagcgg
     2821 actttcgaca acggtagcat tccacatcaa atccacttag gcgaattgca tgctatactt
     2881 agaaggcagg aggattttta tccgttcctc aaagacaatc gtgaaaagat tgagaaaatc
     2941 ctaacctttc gcatacctta ctatgtggga cccctggccc gagggaactc tcggttcgca
     3001 tggatgacaa gaaagtccga agaaacgatt actccatgga attttgagga agttgtcgat
     3061 aaaggtgcgt cagctcaatc gttcatcgag aggatgacca actttgacaa gaatttaccg
     3121 aacgaaaaag tattgcctaa gcacagttta ctttacgagt atttcacagt gtacaatgaa
     3181 ctcacgaaag ttaagtatgt cactgagggc atgcgtaaac ccgcctttct aagcggagaa
     3241 cagaagaaag caatagtaga tctgttattc aagaccaacc gcaaagtgac agttaagcaa
     3301 ttgaaagagg actactttaa gaaaattgaa tgcttcgatt ctgtcgagat ctccggggta
     3361 gaagatcgat ttaatgcgtc acttggtacg tatcatgacc tcctaaagat aattaaagat
     3421 aaggacttcc tggataacga agagaatgaa gatatcttag aagatatagt gttgactctt
     3481 accctctttg aagatcggga aatgattgag gaaagactaa aaacatacgc tcacctgttc
     3541 gacgataagg ttatgaaaca gttaaagagg cgtcgctata cgggctgggg acgattgtcg
     3601 cggaaactta tcaacgggat aagagacaag caaagtggta aaactattct cgattttcta
     3661 aagagcgacg gcttcgccaa taggaacttt atgcagctga tccatgatga ctctttaacc
     3721 ttcaaagagg atatacaaaa ggcacaggtt tccggacaag gggactcatt gcacgaacat
     3781 attgcgaatc ttgctggttc gccagccatc aaaaagggca tactccagac agtcaaagta
     3841 gtggatgagc tagttaaggt catgggacgt cacaaaccgg aaaacattgt aatcgagatg
     3901 gcacgcgaaa atcaaacgac tcagaagggg caaaaaaaca gtcgagagcg gatgaagaga
     3961 atagaagagg gtattaaaga actgggcagc cagatcttaa aggagcatcc tgtggaaaat
     4021 acccaattgc agaacgagaa actttacctc tattacctac aaaatggaag ggacatgtat
     4081 gttgatcagg aactggacat aaaccgttta tctgattacg acgtcgatca cattgtaccc
     4141 caatcctttt tgaaggacga ttcaatcgac aataaagtgc ttacacgctc ggataagaac
     4201 cgagggaaaa gtgacaatgt tccaagcgag gaagtcgtaa agaaaatgaa gaactattgg
     4261 cggcagctcc taaatgcgaa actgataacg caaagaaagt tcgataactt aactaaagct
     4321 gagaggggtg gcttgtctga acttgacaag gccggattta ttaaacgtca gctcgtggaa
     4381 acccgccaaa tcacaaagca tgttgcacag atactagatt cccgaatgaa tacgaaatac
     4441 gacgagaacg ataagctgat tcgggaagtc aaagtaatca ctttaaagtc aaaattggtg
     4501 tcggacttca gaaaggattt tcaattctat aaagttaggg agataaataa ctaccaccat
     4561 gcgcacgacg cttatcttaa tgccgtcgta gggaccgcac tcattaagaa atacccgaag
     4621 ctagaaagtg agtttgtgta tggtgattac aaagtttatg acgtccgtaa gatgatcgcg
     4681 aaaagcgaac aggagatagg caaggctaca gccaaatact tcttttattc taacattatg
     4741 aatttcttta agacggaaat cactctggca aacggagaga tacgcaaacg acctttaatt
     4801 gaaaccaatg gggagacagg tgaaatcgta tgggataagg gccgggactt cgcgacggtg
     4861 agaaaagttt tgtccatgcc ccaagtcaac atagtaaaga aaactgaggt gcagaccgga
     4921 gggttttcaa aggaatcgat tcttccaaaa aggaatagtg ataagctcat cgctcgtaaa
     4981 aaggactggg acccgaaaaa gtacggtggc ttcgatagcc ctacagttgc ctattctgtc
     5041 ctagtagtgg caaaagttga gaagggaaaa tccaagaaac tgaagtcagt caaagaatta
     5101 ttggggataa cgattatgga gcgctcgtct tttgaaaaga accccatcga cttccttgag
     5161 gcgaaaggtt acaaggaagt aaaaaaggat ctcataatta aactaccaaa gtatagtctg
     5221 tttgagttag aaaatggccg aaaacggatg ttggctagcg ccggagagct tcaaaagggg
     5281 aacgaactcg cactaccgtc taaatacgtg aatttcctgt atttagcgtc ccattacgag
     5341 aagttgaaag gttcacctga agataacgaa cagaagcaac tttttgttga gcagcacaaa
     5401 cattatctcg acgaaatcat agagcaaatt tcggaattca gtaagagagt catcctagct
     5461 gatgccaatc tggacaaagt attaagcgca tacaacaagc acagggataa acccatacgt
     5521 gagcaggcgg aaaatattat ccatttgttt actcttacca acctcggcgc tccagccgca
     5581 ttcaagtatt ttgacacaac gatagatcgc aaacgataca cttctaccaa ggaggtgcta
     5641 gacgcgacac tgattcacca atccatcacg ggattatatg aaactcggat agatttgtca
     5701 cagcttgggg gtgactctgg tggttctact aatctgtcag atattattga aaaggagacc
     5761 ggtaagcaac tggttatcca ggaatccatc ctcatgctcc cagaggaggt ggaagaagtc
     5821 attgggaaca agccggaaag cgatatactc gtgcacaccg cctacgacga gagcaccgac
     5881 gagaatgtca tgcttctgac tagcgacgcc cctgaataca agccttgggc tctggtcata
     5941 caggatagca acggtgagaa caagattaag atgctctctg gtggttctcc caagaagaag
     6001 aggaaagtct aaccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     6061 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6121 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6181 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6241 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6301 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
     6361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6421 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7201 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7321 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7441 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7501 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7561 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7621 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7681 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7741 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7801 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7861 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7921 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7981 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8041 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8101 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8161 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8221 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8281 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8341 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8401 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8461 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8521 gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg
     8581 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     8641 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     8701 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     8761 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     8821 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     8881 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     8941 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     9001 atc
//
