LOCUS       sequence-213965-        8964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hA3F-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-hA3F-BE3
FEATURES             Location/Qualifiers
     source          1..8964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1576..4974,4975..4977,4978..5574,5575,5576..5578,
                     5579..5580,5581..5583,5584..5676)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1576..1599,1600..1602,1603..5676)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5689..5937
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5950..5970
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5979..5996
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6019..6036)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6025..6249
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6320..6336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6320..6336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6333..6355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6344..6360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6368..6374,6375..6392,6393..6398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6413..6434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6551..6568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6722..7310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6802..6821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7481..8272,8273..8341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8104..8123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8342..8446)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8525..8544)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8716..8964,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaagcctcac
      421 ttcagaaaca cagtggagcg aatgtatcga gacacattct cctacaactt ttataataga
      481 cccatccttt ctcgtcggaa taccgtctgg ctgtgctacg aagtgaaaac aaagggtccc
      541 tcaaggcccc gtttggacgc aaagatcttt cgaggccagg tgtattccca gcctgagcac
      601 cacgcagaaa tgtgcttcct ctcttggttc tgtggcaacc agctgcctgc ttacaagtgt
      661 ttccagatca cctggtttgt atcctggacc ccctgcccgg actgtgtggc gaagctggcc
      721 gaattcctgg ctgagcaccc caatgtcacc ctgaccatct ccgccgcccg cctctactac
      781 tactgggaaa gagattaccg aagggcgctc tgcaggctga gtcaggcagg ggcccgcgtg
      841 aagattatgg acgatgaaga atttgcatac tgctgggaaa actttgtgta cagtgaaggt
      901 cagccattca tgccttggta caaattcgat gacaattatg cattcctgca ccgcacgcta
      961 aaggagattc tcagaaaccc gatggaggca atgtatccac acatattcta cttccacttt
     1021 aaaaacctac gcaaagccta tggtcggaac gaaagctggc tgtgcttcac catggaagtt
     1081 gtaaagcacc actcacctgt ctcctggaag aggggcgtct tccgaaacca ggtggatcct
     1141 gagacccatt gtcatgcaga aaggtgcttc ctctcttggt tctgtgacga catactgtct
     1201 cctaacacaa actacgaggt cacctggtac acatcttgga gcccttgccc agagtgtgca
     1261 ggggaggtgg ccgagttcct ggccaggcac agcaacgtga atctcaccat cttcaccgcc
     1321 cgcctctact acttctggga tacagattac caggaggggc tccgcagcct gagtcaggaa
     1381 ggggcctccg tggagatcat gggctacaaa gattttaaat attgttggga aaactttgtg
     1441 tacaatgatg atgagccatt caagccttgg aaaggactaa aatacaactt tctattcctg
     1501 gacagcaagc tgcaggagat tctcgagagc ggcagcgaga ctcccgggac ctcagagtcc
     1561 gccacacccg aaagtgataa aaagtattct attggtttag ccatcggcac taattccgtt
     1621 ggatgggctg tcataaccga tgaatacaaa gtaccttcaa agaaatttaa ggtgttgggg
     1681 aacacagacc gtcattcgat taaaaagaat cttatcggtg ccctcctatt cgatagtggc
     1741 gaaacggcag aggcgactcg cctgaaacga accgctcgga gaaggtatac acgtcgcaag
     1801 aaccgaatat gttacttaca agaaattttt agcaatgaga tggccaaagt tgacgattct
     1861 ttctttcacc gtttggaaga gtccttcctt gtcgaagagg acaagaaaca tgaacggcac
     1921 cccatctttg gaaacatagt agatgaggtg gcatatcatg aaaagtaccc aacgatttat
     1981 cacctcagaa aaaagctagt tgactcaact gataaagcgg acctgaggtt aatctacttg
     2041 gctcttgccc atatgataaa gttccgtggg cactttctca ttgagggtga tctaaatccg
     2101 gacaactcgg atgtcgacaa actgttcatc cagttagtac aaacctataa tcagttgttt
     2161 gaagagaacc ctataaatgc aagtggcgtg gatgcgaagg ctattcttag cgcccgcctc
     2221 tctaaatccc gacggctaga aaacctgatc gcacaattac ccggagagaa gaaaaatggg
     2281 ttgttcggta accttatagc gctctcacta ggcctgacac caaattttaa gtcgaacttc
     2341 gacttagctg aagatgccaa attgcagctt agtaaggaca cgtacgatga cgatctcgac
     2401 aatctactgg cacaaattgg agatcagtat gcggacttat ttttggctgc caaaaacctt
     2461 agcgatgcaa tcctcctatc tgacatactg agagttaata ctgagattac caaggcgccg
     2521 ttatccgctt caatgatcaa aaggtacgat gaacatcacc aagacttgac acttctcaag
     2581 gccctagtcc gtcagcaact gcctgagaaa tataaggaaa tattctttga tcagtcgaaa
     2641 aacgggtacg caggttatat tgacggcgga gcgagtcaag aggaattcta caagtttatc
     2701 aaacccatat tagagaagat ggatgggacg gaagagttgc ttgtaaaact caatcgcgaa
     2761 gatctactgc gaaagcagcg gactttcgac aacggtagca ttccacatca aatccactta
     2821 ggcgaattgc atgctatact tagaaggcag gaggattttt atccgttcct caaagacaat
     2881 cgtgaaaaga ttgagaaaat cctaaccttt cgcatacctt actatgtggg acccctggcc
     2941 cgagggaact ctcggttcgc atggatgaca agaaagtccg aagaaacgat tactccatgg
     3001 aattttgagg aagttgtcga taaaggtgcg tcagctcaat cgttcatcga gaggatgacc
     3061 aactttgaca agaatttacc gaacgaaaaa gtattgccta agcacagttt actttacgag
     3121 tatttcacag tgtacaatga actcacgaaa gttaagtatg tcactgaggg catgcgtaaa
     3181 cccgcctttc taagcggaga acagaagaaa gcaatagtag atctgttatt caagaccaac
     3241 cgcaaagtga cagttaagca attgaaagag gactacttta agaaaattga atgcttcgat
     3301 tctgtcgaga tctccggggt agaagatcga tttaatgcgt cacttggtac gtatcatgac
     3361 ctcctaaaga taattaaaga taaggacttc ctggataacg aagagaatga agatatctta
     3421 gaagatatag tgttgactct taccctcttt gaagatcggg aaatgattga ggaaagacta
     3481 aaaacatacg ctcacctgtt cgacgataag gttatgaaac agttaaagag gcgtcgctat
     3541 acgggctggg gacgattgtc gcggaaactt atcaacggga taagagacaa gcaaagtggt
     3601 aaaactattc tcgattttct aaagagcgac ggcttcgcca ataggaactt tatgcagctg
     3661 atccatgatg actctttaac cttcaaagag gatatacaaa aggcacaggt ttccggacaa
     3721 ggggactcat tgcacgaaca tattgcgaat cttgctggtt cgccagccat caaaaagggc
     3781 atactccaga cagtcaaagt agtggatgag ctagttaagg tcatgggacg tcacaaaccg
     3841 gaaaacattg taatcgagat ggcacgcgaa aatcaaacga ctcagaaggg gcaaaaaaac
     3901 agtcgagagc ggatgaagag aatagaagag ggtattaaag aactgggcag ccagatctta
     3961 aaggagcatc ctgtggaaaa tacccaattg cagaacgaga aactttacct ctattaccta
     4021 caaaatggaa gggacatgta tgttgatcag gaactggaca taaaccgttt atctgattac
     4081 gacgtcgatc acattgtacc ccaatccttt ttgaaggacg attcaatcga caataaagtg
     4141 cttacacgct cggataagaa ccgagggaaa agtgacaatg ttccaagcga ggaagtcgta
     4201 aagaaaatga agaactattg gcggcagctc ctaaatgcga aactgataac gcaaagaaag
     4261 ttcgataact taactaaagc tgagaggggt ggcttgtctg aacttgacaa ggccggattt
     4321 attaaacgtc agctcgtgga aacccgccaa atcacaaagc atgttgcaca gatactagat
     4381 tcccgaatga atacgaaata cgacgagaac gataagctga ttcgggaagt caaagtaatc
     4441 actttaaagt caaaattggt gtcggacttc agaaaggatt ttcaattcta taaagttagg
     4501 gagataaata actaccacca tgcgcacgac gcttatctta atgccgtcgt agggaccgca
     4561 ctcattaaga aatacccgaa gctagaaagt gagtttgtgt atggtgatta caaagtttat
     4621 gacgtccgta agatgatcgc gaaaagcgaa caggagatag gcaaggctac agccaaatac
     4681 ttcttttatt ctaacattat gaatttcttt aagacggaaa tcactctggc aaacggagag
     4741 atacgcaaac gacctttaat tgaaaccaat ggggagacag gtgaaatcgt atgggataag
     4801 ggccgggact tcgcgacggt gagaaaagtt ttgtccatgc cccaagtcaa catagtaaag
     4861 aaaactgagg tgcagaccgg agggttttca aaggaatcga ttcttccaaa aaggaatagt
     4921 gataagctca tcgctcgtaa aaaggactgg gacccgaaaa agtacggtgg cttcgatagc
     4981 cctacagttg cctattctgt cctagtagtg gcaaaagttg agaagggaaa atccaagaaa
     5041 ctgaagtcag tcaaagaatt attggggata acgattatgg agcgctcgtc ttttgaaaag
     5101 aaccccatcg acttccttga ggcgaaaggt tacaaggaag taaaaaagga tctcataatt
     5161 aaactaccaa agtatagtct gtttgagtta gaaaatggcc gaaaacggat gttggctagc
     5221 gccggagagc ttcaaaaggg gaacgaactc gcactaccgt ctaaatacgt gaatttcctg
     5281 tatttagcgt cccattacga gaagttgaaa ggttcacctg aagataacga acagaagcaa
     5341 ctttttgttg agcagcacaa acattatctc gacgaaatca tagagcaaat ttcggaattc
     5401 agtaagagag tcatcctagc tgatgccaat ctggacaaag tattaagcgc atacaacaag
     5461 cacagggata aacccatacg tgagcaggcg gaaaatatta tccatttgtt tactcttacc
     5521 aacctcggcg ctccagccgc attcaagtat tttgacacaa cgatagatcg caaacgatac
     5581 acttctacca aggaggtgct agacgcgaca ctgattcacc aatccatcac gggattatat
     5641 gaaactcgga tagatttgtc acagcttggg ggtgactctg gtggttctac taatctgtca
     5701 gatattattg aaaaggagac cggtaagcaa ctggttatcc aggaatccat cctcatgctc
     5761 ccagaggagg tggaagaagt cattgggaac aagccggaaa gcgatatact cgtgcacacc
     5821 gcctacgacg agagcaccga cgagaatgtc atgcttctga ctagcgacgc ccctgaatac
     5881 aagccttggg ctctggtcat acaggatagc aacggtgaga acaagattaa gatgctctct
     5941 ggtggttctc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat caccattgag
     6001 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6061 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6121 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6181 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6241 gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct
     6301 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga
     6421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6481 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6541 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7141 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8461 aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc
     8521 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     8581 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     8641 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     8701 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     8761 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     8821 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     8881 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     8941 acttggcagt acatcaagtg tatc
//
