LOCUS       sequence-213967-        8454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hA3H-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-hA3H-BE3
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1066..4464,4465..4467,4468..5064,5065,5066..5068,
                     5069..5070,5071..5073,5074..5166)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1066..1089,1090..1092,1093..5166)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5179..5427
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5440..5460
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5469..5486
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5509..5526)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5515..5739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5810..5826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5810..5826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5823..5845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5834..5850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5858..5864,5865..5882,5883..5888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5903..5924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6041..6058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6212..6800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6292..6311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6971..7762,7763..7831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7594..7613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7832..7936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8015..8034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8206..8454,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggctctgtta
      421 acagccgaaa cattccgctt acagtttaac aacaagcgcc gcctcagaag gccttactac
      481 ccgaggaagg ccctcttgtg ttaccagctg acgccgcaga atggctccac gcccacgaga
      541 ggctactttg aaaacaagaa aaagtgccat gcagaaattt gctttattaa cgagatcaag
      601 tccatgggac tggacgaaac gcagtgctac caagtcacct gttacctcac gtggagcccc
      661 tgctcctcct gtgcctggga gctggttgac ttcatcaagg ctcacgacca tctgaacctg
      721 ggcatcttcg cctcccgcct gtactaccac tggtgcaagc cccagcagaa ggggctgcgg
      781 cttctgtgtg gatcccaggt cccggtggag gtcatgggct tcccagagtt tgctgactgc
      841 tgggaaaact ttgtggacca cgagaaaccg ctttccttca acccctataa gatgttagag
      901 gagctagata aaaacagtcg agccataaag cgacggcttg agaggataaa ggggctcctt
      961 cttgttcttt tagattccag gggtacgtgc gcagggtcgt tacatggata tattgtgagc
     1021 ggcagcgaga ctcccgggac ctcagagtcc gccacacccg aaagtgataa aaagtattct
     1081 attggtttag ccatcggcac taattccgtt ggatgggctg tcataaccga tgaatacaaa
     1141 gtaccttcaa agaaatttaa ggtgttgggg aacacagacc gtcattcgat taaaaagaat
     1201 cttatcggtg ccctcctatt cgatagtggc gaaacggcag aggcgactcg cctgaaacga
     1261 accgctcgga gaaggtatac acgtcgcaag aaccgaatat gttacttaca agaaattttt
     1321 agcaatgaga tggccaaagt tgacgattct ttctttcacc gtttggaaga gtccttcctt
     1381 gtcgaagagg acaagaaaca tgaacggcac cccatctttg gaaacatagt agatgaggtg
     1441 gcatatcatg aaaagtaccc aacgatttat cacctcagaa aaaagctagt tgactcaact
     1501 gataaagcgg acctgaggtt aatctacttg gctcttgccc atatgataaa gttccgtggg
     1561 cactttctca ttgagggtga tctaaatccg gacaactcgg atgtcgacaa actgttcatc
     1621 cagttagtac aaacctataa tcagttgttt gaagagaacc ctataaatgc aagtggcgtg
     1681 gatgcgaagg ctattcttag cgcccgcctc tctaaatccc gacggctaga aaacctgatc
     1741 gcacaattac ccggagagaa gaaaaatggg ttgttcggta accttatagc gctctcacta
     1801 ggcctgacac caaattttaa gtcgaacttc gacttagctg aagatgccaa attgcagctt
     1861 agtaaggaca cgtacgatga cgatctcgac aatctactgg cacaaattgg agatcagtat
     1921 gcggacttat ttttggctgc caaaaacctt agcgatgcaa tcctcctatc tgacatactg
     1981 agagttaata ctgagattac caaggcgccg ttatccgctt caatgatcaa aaggtacgat
     2041 gaacatcacc aagacttgac acttctcaag gccctagtcc gtcagcaact gcctgagaaa
     2101 tataaggaaa tattctttga tcagtcgaaa aacgggtacg caggttatat tgacggcgga
     2161 gcgagtcaag aggaattcta caagtttatc aaacccatat tagagaagat ggatgggacg
     2221 gaagagttgc ttgtaaaact caatcgcgaa gatctactgc gaaagcagcg gactttcgac
     2281 aacggtagca ttccacatca aatccactta ggcgaattgc atgctatact tagaaggcag
     2341 gaggattttt atccgttcct caaagacaat cgtgaaaaga ttgagaaaat cctaaccttt
     2401 cgcatacctt actatgtggg acccctggcc cgagggaact ctcggttcgc atggatgaca
     2461 agaaagtccg aagaaacgat tactccatgg aattttgagg aagttgtcga taaaggtgcg
     2521 tcagctcaat cgttcatcga gaggatgacc aactttgaca agaatttacc gaacgaaaaa
     2581 gtattgccta agcacagttt actttacgag tatttcacag tgtacaatga actcacgaaa
     2641 gttaagtatg tcactgaggg catgcgtaaa cccgcctttc taagcggaga acagaagaaa
     2701 gcaatagtag atctgttatt caagaccaac cgcaaagtga cagttaagca attgaaagag
     2761 gactacttta agaaaattga atgcttcgat tctgtcgaga tctccggggt agaagatcga
     2821 tttaatgcgt cacttggtac gtatcatgac ctcctaaaga taattaaaga taaggacttc
     2881 ctggataacg aagagaatga agatatctta gaagatatag tgttgactct taccctcttt
     2941 gaagatcggg aaatgattga ggaaagacta aaaacatacg ctcacctgtt cgacgataag
     3001 gttatgaaac agttaaagag gcgtcgctat acgggctggg gacgattgtc gcggaaactt
     3061 atcaacggga taagagacaa gcaaagtggt aaaactattc tcgattttct aaagagcgac
     3121 ggcttcgcca ataggaactt tatgcagctg atccatgatg actctttaac cttcaaagag
     3181 gatatacaaa aggcacaggt ttccggacaa ggggactcat tgcacgaaca tattgcgaat
     3241 cttgctggtt cgccagccat caaaaagggc atactccaga cagtcaaagt agtggatgag
     3301 ctagttaagg tcatgggacg tcacaaaccg gaaaacattg taatcgagat ggcacgcgaa
     3361 aatcaaacga ctcagaaggg gcaaaaaaac agtcgagagc ggatgaagag aatagaagag
     3421 ggtattaaag aactgggcag ccagatctta aaggagcatc ctgtggaaaa tacccaattg
     3481 cagaacgaga aactttacct ctattaccta caaaatggaa gggacatgta tgttgatcag
     3541 gaactggaca taaaccgttt atctgattac gacgtcgatc acattgtacc ccaatccttt
     3601 ttgaaggacg attcaatcga caataaagtg cttacacgct cggataagaa ccgagggaaa
     3661 agtgacaatg ttccaagcga ggaagtcgta aagaaaatga agaactattg gcggcagctc
     3721 ctaaatgcga aactgataac gcaaagaaag ttcgataact taactaaagc tgagaggggt
     3781 ggcttgtctg aacttgacaa ggccggattt attaaacgtc agctcgtgga aacccgccaa
     3841 atcacaaagc atgttgcaca gatactagat tcccgaatga atacgaaata cgacgagaac
     3901 gataagctga ttcgggaagt caaagtaatc actttaaagt caaaattggt gtcggacttc
     3961 agaaaggatt ttcaattcta taaagttagg gagataaata actaccacca tgcgcacgac
     4021 gcttatctta atgccgtcgt agggaccgca ctcattaaga aatacccgaa gctagaaagt
     4081 gagtttgtgt atggtgatta caaagtttat gacgtccgta agatgatcgc gaaaagcgaa
     4141 caggagatag gcaaggctac agccaaatac ttcttttatt ctaacattat gaatttcttt
     4201 aagacggaaa tcactctggc aaacggagag atacgcaaac gacctttaat tgaaaccaat
     4261 ggggagacag gtgaaatcgt atgggataag ggccgggact tcgcgacggt gagaaaagtt
     4321 ttgtccatgc cccaagtcaa catagtaaag aaaactgagg tgcagaccgg agggttttca
     4381 aaggaatcga ttcttccaaa aaggaatagt gataagctca tcgctcgtaa aaaggactgg
     4441 gacccgaaaa agtacggtgg cttcgatagc cctacagttg cctattctgt cctagtagtg
     4501 gcaaaagttg agaagggaaa atccaagaaa ctgaagtcag tcaaagaatt attggggata
     4561 acgattatgg agcgctcgtc ttttgaaaag aaccccatcg acttccttga ggcgaaaggt
     4621 tacaaggaag taaaaaagga tctcataatt aaactaccaa agtatagtct gtttgagtta
     4681 gaaaatggcc gaaaacggat gttggctagc gccggagagc ttcaaaaggg gaacgaactc
     4741 gcactaccgt ctaaatacgt gaatttcctg tatttagcgt cccattacga gaagttgaaa
     4801 ggttcacctg aagataacga acagaagcaa ctttttgttg agcagcacaa acattatctc
     4861 gacgaaatca tagagcaaat ttcggaattc agtaagagag tcatcctagc tgatgccaat
     4921 ctggacaaag tattaagcgc atacaacaag cacagggata aacccatacg tgagcaggcg
     4981 gaaaatatta tccatttgtt tactcttacc aacctcggcg ctccagccgc attcaagtat
     5041 tttgacacaa cgatagatcg caaacgatac acttctacca aggaggtgct agacgcgaca
     5101 ctgattcacc aatccatcac gggattatat gaaactcgga tagatttgtc acagcttggg
     5161 ggtgactctg gtggttctac taatctgtca gatattattg aaaaggagac cggtaagcaa
     5221 ctggttatcc aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac
     5281 aagccggaaa gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc
     5341 atgcttctga ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc
     5401 aacggtgaga acaagattaa gatgctctct ggtggttctc ccaagaagaa gaggaaagtc
     5461 taaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     5521 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5581 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5641 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5701 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5761 gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5821 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5881 agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5941 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6001 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6061 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6121 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6181 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6241 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6301 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6361 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6421 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6481 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6541 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6601 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6661 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6721 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6781 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6841 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6901 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6961 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7021 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7081 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7141 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7201 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7261 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7321 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7381 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7441 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7501 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7561 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7621 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7681 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7741 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7801 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7861 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7921 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     7981 atcgatctcc cgatccccta gggtcgactc tcagtacaat ctgctctgat gccgcatagt
     8041 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8101 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     8161 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     8221 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     8281 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     8341 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     8401 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatc
//
