LOCUS       sequence-213970-        9132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mA3-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-mA3-BE3
FEATURES             Location/Qualifiers
     source          1..9132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1744..5142,5143..5145,5146..5742,5743,5744..5746,
                     5747..5748,5749..5751,5752..5844)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1744..1767,1768..1770,1771..5844)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5857..6105
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6118..6138
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6147..6164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6187..6204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6193..6417
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6488..6504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6488..6504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6501..6523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6512..6528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6536..6542,6543..6560,6561..6566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6581..6602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6719..6736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6890..7478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6970..6989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7649..8440,8441..8509))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8272..8291
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8510..8614)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8693..8712)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8884..9132,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gggaccattc
      421 tgtctgggat gcagccatcg caaatgctat tcaccgatca gaaacctgat atctcaagaa
      481 acattcaagt tccactttaa gaacctaggc tatgccaaag gccggaaaga taccttcttg
      541 tgctatgaag tgactagaaa ggactgcgat tcacccgtct cccttcacca tggggtcttt
      601 aagaacaagg acaacatcca cgctgaaatc tgctttttat actggttcca tgacaaagta
      661 ctgaaagtgc tgtctccgag agaagagttc aagatcacct ggtatatgtc ctggagcccc
      721 tgtttcgaat gtgcagagca gatagtaagg ttcctggcta cacaccacaa cctgagcctg
      781 gacatcttca gctcccgcct ctacaacgta caggacccag aaacccagca gaatctttgc
      841 aggctggttc aggaaggagc ccaggtggct gccatggacc tatacgaatt taaaaagtgt
      901 tggaagaagt ttgtggacaa tggtggcagg cgattcaggc cttggaaaag actgcttaca
      961 aattttagat accaggattc taagcttcag gagattctga gaccttgcta catctcggtc
     1021 ccttccagct cttcatccac tctgtcaaat atctgtctaa caaaaggtct cccagagacg
     1081 aggttctggg tggagggcag gcgaatggac ccgctaagtg aagaggaatt ttactcgcag
     1141 ttttacaacc aacgagtcaa gcatctctgc tactaccacc gcatgaagcc ctatctatgc
     1201 taccagctgg agcagttcaa tggccaagcg ccactcaaag gctgcctgct aagcgagaaa
     1261 ggcaaacagc atgcagaaat cctcttcctt gataagattc ggtccatgga gctgagccaa
     1321 gtgacaatca cctgctacct cacctggagc ccctgcccaa actgtgcctg gcaactggcg
     1381 gcattcaaaa gggatcgtcc agatctaatt ctgcatatct acacctcccg cctgtatttc
     1441 cactggaaga ggcccttcca gaaggggctg tgttctctgt ggcaatcagg gatcctggtg
     1501 gacgtcatgg acctcccaca gtttactgac tgctggacaa actttgtgaa cccgaaaagg
     1561 ccgttttggc catggaaagg attggagata atcagcaggc gcacacaaag gcggctccgc
     1621 aggatcaagg agtcctgggg tctgcaagat ttggtgaatg actttggaaa cctacagctt
     1681 ggacccccga tgtctagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa
     1741 agtgataaaa agtattctat tggtttagcc atcggcacta attccgttgg atgggctgtc
     1801 ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt
     1861 cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag
     1921 gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt
     1981 tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt
     2041 ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga
     2101 aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa
     2161 aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat
     2221 atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat
     2281 gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct
     2341 ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga
     2401 cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac
     2461 cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa
     2521 gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca
     2581 caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc
     2641 ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca
     2701 atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt
     2761 cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca
     2821 ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa acccatatta
     2881 gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga
     2941 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat
     3001 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt
     3061 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct
     3121 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa
     3181 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag
     3241 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg
     3301 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta
     3361 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca
     3421 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc
     3481 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata
     3541 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg
     3601 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct
     3661 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga
     3721 cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc
     3781 gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac
     3841 tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg
     3901 cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca
     3961 gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta
     4021 atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg
     4081 atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct
     4141 gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg
     4201 gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatcac
     4261 attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg
     4321 gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag
     4381 aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta
     4441 actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat taaacgtcag
     4501 ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc ccgaatgaat
     4561 acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac tttaaagtca
     4621 aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga gataaataac
     4681 taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact cattaagaaa
     4741 tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga cgtccgtaag
     4801 atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt cttttattct
     4861 aacattatga atttctttaa gacggaaatc actctggcaa acggagagat acgcaaacga
     4921 cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg ccgggacttc
     4981 gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa aactgaggtg
     5041 cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga taagctcatc
     5101 gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc tacagttgcc
     5161 tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact gaagtcagtc
     5221 aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa ccccatcgac
     5281 ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa actaccaaag
     5341 tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc cggagagctt
     5401 caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta tttagcgtcc
     5461 cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact ttttgttgag
     5521 cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag taagagagtc
     5581 atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca cagggataaa
     5641 cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa cctcggcgct
     5701 ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac ttctaccaag
     5761 gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga aactcggata
     5821 gatttgtcac agcttggggg tgactctggt ggttctacta atctgtcaga tattattgaa
     5881 aaggagaccg gtaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg
     5941 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag
     6001 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct
     6061 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc
     6121 aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt taaacccgct
     6181 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6241 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6301 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6361 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     6421 ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt
     6481 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6541 tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat
     6601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6661 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7321 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8641 acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct
     8701 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8761 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     8821 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     8881 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     8941 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9001 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9061 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     9121 atcaagtgta tc
//
