LOCUS       sequence-213994-        7028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  4 kb HindII rat genomic fragment containing the entire VGF gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7028)
  TITLE     Levi Lab plasmids
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Hg
FEATURES             Location/Qualifiers
     source          1..7028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    102..123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(138..143,144..161,162..168)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    176..192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     181..203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     200..216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     200..216
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4628..4645)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4628..4644)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4637..4659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4853..4872)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4972..4994
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5032..5050)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5118..5222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5223..5291,5292..6083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5441..5460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6254..6842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6743..6762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6996..7013
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
       61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      121 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      181 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc gagctcgccc
      241 ggggatcgat cctctagagt cgacctgcag cccaagcttg cttattccaa ccaactggct
      301 cttttcttgt ttgttttgtt ccccaacccc aactcctacc aactgagcca cgtccccact
      361 cccaacaact ggttcttgtt ggactccctt ctttcctggg tcaaaaactg ggaacaaggt
      421 ggaagtggaa aaagtagggt tgtattttgt tatggttttt attacatttg ttttgtgggt
      481 gggtgagcgt accaaggtgg gcatgtggaa gtcagggaga gaacttgcag tggtcagtag
      541 gaaggtaccc tggtatgaca ctcatgtctt gcttggcaac gtgtaccttt tactagaaca
      601 ttccctttcc aggttcacct tcactccttt ctcccaccca gtctggcttt ggtctgagac
      661 tagggagact ggcgcttccc tcaccgcccc ttggaaagga ccagataggc ccaaggggac
      721 gggtgaataa tggaaaacaa agtcctaata aaatagtcac ttttatttct tagcaaaact
      781 atttcctccg tgaggggtat ttacaacaga agggcaagat gtgggtaagt tcacagcaat
      841 ttggagaagg gaggctccgg gggccccaag gctgggagac agacacttca cacgattaaa
      901 tagaacagct tttccccgtt tgggaccagg aagtcgctag agaaactaga tggggacgag
      961 gcccctggag gaagtggggg tgtaagggga ctcgcacaaa ccacacaccc gcctgggctg
     1021 gccccaggat cagaccccaa agaagtctga caggtctggt cctgacagaa cccggggcgg
     1081 ggccttgtgg ggcggggccg ggcggcagcc gaggggcgga gccggggcgc atgcaaacac
     1141 cgagagggag gaggagcgac acggaggggg ggcgtggcgg cgcgcgcagt tgggagcggg
     1201 cgcgcagggg cgggtcacgg gcggtgcagc agcacgtgct caatgtaatt ctccagctcc
     1261 tcctgctcct gcagccggcg ctcctccgcg tccgcttcct cctgcgcgcg cctggcctgg
     1321 gcctccagat cgggatggtg gcgcgcaggt ggcaacgcgt gatggaagtg acggcggcgg
     1381 gaggatgcgg gcggctgcag tgtccgcggc cgaatgtagt ttgggaaggg gtgatagggc
     1441 cccgggggaa acacctcatc ctcctcccga tcccagggtg ggagtacttc gttccagtcc
     1501 ggcaactcat cccgggccgg ggcgggaggt gggggctgcg gggagcggac atgggtcggg
     1561 gctggggcag ccctgggggg cggcaccggc tcgggagggg cgttcttctt ccgcttccgt
     1621 ttctcctcca cctcttcgat gatgctgacc acatcgtctg ctggcaggtg gagtttggtg
     1681 gacagttcaa tgagactatc gatcgtctgc ggatccatct cttcgtcgtc gtcatcctcc
     1741 tccccgccct cctctgtccc ctcagcatcc ttccgccgat ggcctggcgc ctcctcctgg
     1801 gagcgcttgt cctcggctcc ggcttccccg tcctcctcct cggcgaacag gagcgcgttc
     1861 tgccgcgccc tctccgcctc ctctgcctcc gcctccgcct ctgccgcctc ctcatcctct
     1921 tcccccacct cgtcctcccc accaccgcgt ctctcctgct ccgcctcctc ctccctctcg
     1981 ttctcccgct cttgctgcgt ctcctgcagc ccgcgacccc cgagatcgcg ctgccgggcg
     2041 ccgccctgca gcaaatactg gagcagcagg tcggaggcga gatcggccag ccgctcttct
     2101 tgcgctgcgg cctgccgggt ggcctccgcc tgcctcctcc ctgcctctac ctgagctaac
     2161 ccttgttgaa gcaggcgctc tcctgcctcg gaaccgccca ggaatgagcc ctcgagccga
     2221 cgcaccttgg ggaagggggc acttagactt tggtacgcct tggataaggg tgtcaaagtc
     2281 tcaccaagat gtgttttagg ggaggacact ccttccccga actgatgggt ttcgggaagg
     2341 ggaacgtttt cggacattcg agcctggaat tgagaaggga ccgagggcgg cagaggagca
     2401 cgctccggga cgcgcgcctg gaactctccc caggaagcgc gccatacgcg ctctggcccg
     2461 gggctctcca gattgactcg ggtcagcgtg tgcgtgcggg tttcagtctc tgctgccgcc
     2521 gtctcttgct ggcgcttagc attactcgga ctgaaatctc gaagttcttg gagcaaggat
     2581 gctagtgcct ccagctcttc tgagcggtcg tctgcctcgg gatcgttgtc ctgagtctga
     2641 gggcgagggg gcgccacagc ggacgcttgg atttctgatg ccgggaggct atgggtctga
     2701 ctgcgcacgg actcggtcag cagagcttct gctgcttctt cgggtgttcc ctgctgggaa
     2761 cctgccggga cagccggggg cgaggccgga cggtccagtg cctgcaacag taccgcggcc
     2821 agcgcccggg gatccacgcc ctggaagagc tctccctggt cctgaggctc ggaattccga
     2881 gccgctcgga cctctgggac gctgtcatcc tttggccggg acactgcgtc ctcagctacc
     2941 tgcccattat gctcagagcc gaggggagga ggataaacat cggagcgccc ggggggtgct
     3001 gctcccaacc cccggatgag tagaaggaag cagaagagga cggatgctgg caacgtgaag
     3061 gttttcatga ccaacgggct gccagagact gaaaaacaga agaaaccaga gaaatagagg
     3121 atttctataa gaatttccct ctttctattt ctgcattccc tccccccacc tttaagcagg
     3181 aaatccgggg tttccctcat ttctctaacc ttacccgaag agaaacgttt agcagagaac
     3241 aagaaagatg tggcaatctc tggggtcgtg caaatggtca gagaacgctc gcaatcctcg
     3301 ttctgggtac ccagtgcctc ctgtcgcccc cttcccaaag tcatctctca gccagcgccc
     3361 gtgtactaag cagcaatgat aagtgggagg ggggatctgt tccctcccca caggactccc
     3421 cggatggtgc gtgggcgact tcatcagcga cagagaagca gagactcccc gtttaccaaa
     3481 gacccttccc gggggcacac cgctatgctc tcaatacccc ataaggaaag aaaatcctcc
     3541 tttcacgccc acgagtgaag agggttagag ggatggacag cggagtattt accagccggt
     3601 gtcacgacgg cgggaggtgg agaggagggt cggggcaggg ggaaaatccg gacccgtctg
     3661 ccctggctcc gttccgtggc tggagtatga aagatggtcg agggctggcg tcctaggagc
     3721 tgggctcagc tgggtcagca acagcgctct cgctccggct tcagcacgct ggacagcgcc
     3781 cgcgccaccg ctgctttata aaggggagcg cgcggggtca cgtggtctcg ccccgcccca
     3841 ttgacgtcaa tgttcattca tggggaagcg ggcgggtgcc tggtggactg tggaagtcca
     3901 atgattggag gataagcgtc gctcccggcc tgcacctgcg cagtagggcc ctcggctaga
     3961 ggggcgtgag ctagcggagc tccgggaaat gaatgaatga atgaatgaat gaaatgtcta
     4021 ggcgggcggg gcagggggct atgaatgaat gaaggaggga cgaggagaaa aggatgaatg
     4081 aatgaatgga aaaatgaatg gaagggtggg tacagaggag ccagaaggcg ggagggtggg
     4141 ttacaaagct cttgaggcgc ggagcccgcg ttgctgagca gaataggaag agggcaccgc
     4201 cagcatctaa ttgggcgcca gtaaaagatc agtactggat ggggggacgg taagggattg
     4261 aaagccgggg aaggggcctg cctccctact ccgccacagt atggtctata gggagcacgc
     4321 ccaaggacga cttgaacctg agaggaagag ggggtggatc tgcggtgtgg gatgcccgcc
     4381 cagggtcagc gcagatgggt ggatcttgcg gtttgggtaa tcaccttgga aagggtccta
     4441 gtcggaagag aaaagggaaa gattgacgat acgtgtgcct ctgggtcctg tctgttcaaa
     4501 ccagttctga agggggtggg gaaaaaaggt gtgtctgccc ttctggccag cggactttgc
     4561 tgtggttgat aaggggtccc tgttgtgccc ccttttaatc caactctccc acacccttaa
     4621 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4681 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4741 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     4801 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     4861 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4921 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4981 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5041 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5101 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5161 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5221 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5281 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5341 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5401 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5461 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5521 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5581 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5641 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5701 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5761 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5821 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5881 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5941 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6001 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6061 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6121 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6181 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6421 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7021 aagagcgc
//
