LOCUS       sequence-214051-       10808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OGDH ORF containing silent wobble mutations insensitive to 
            corresponding shRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Ilic N, Birsoy K, Aguirre AJ, Kory N, Pacold ME, Singh S, Moody SE, 
            DeAngelo JD, Spardy NA, Freinkman E, Weir BA, Tsherniak A, Cowley 
            GS, Root DE, Asara JM, Vazquez F, Widlund HR, Sabatini DM, Hahn WC
  TITLE     PIK3CA mutant tumors depend on oxoglutarate dehydrogenase.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3434-E3443. doi: 
            10.1073/pnas.1617922114. Epub 2017 Apr 10.
  PUBMED    28396387
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1617922114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-04-25- 25"; volume: "114"; issue: "17"; pages: 
            "E3434-E3443"
COMMENT     Sequence Label: pLenti6.3-OGDH_wobble
FEATURES             Location/Qualifiers
     source          1..10808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    899..920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(935..940,941..958,959..965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    973..989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     978..1000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     997..1013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     997..1013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1032..1052
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1034..1052
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1080..1306
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1307..1487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1534..1659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2152..2385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2570..2614
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2881..2998
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3021..3324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3325..3528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3479..3499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3525..3549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3645..3669
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6746..6770)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6823..6864
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6888..7476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6941..6961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7359..7370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7491..7807
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7658..7793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7855..7902
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7921..8319
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8406..8639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8711..8832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8748..8767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8802..8821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8872..9007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9027..9046)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9028..9046)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9056..9073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9056..9072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9065..9087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9214..9669
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9301..9320)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9511..9532
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9695..9799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9800..9868,9869..10660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10018..10037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      961 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     1021 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     1081 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     1141 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     1201 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     1261 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     1321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1501 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1561 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1621 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1681 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1741 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1801 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1861 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1921 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1981 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2041 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2101 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2161 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2221 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2281 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2341 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2401 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2461 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2521 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2581 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2641 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2701 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2761 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2821 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     2881 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2941 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat
     3001 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3061 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3541 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     3601 tagaggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
     3661 aagttggctc caccatgttt catttaagga cttgtgctgc taagttgaga ccattgacgg
     3721 cttcccagac tgttaagaca ttttcacaaa acagaccagc agcagctagg acatttcaac
     3781 agattcggtg ctattctgca cctgttgctg ctgagccctt tctcagtggg actagttcga
     3841 actatgtgga ggagatgtac tgtgcttggc tggaaaaccc caaaagtgta cataagtcat
     3901 gggacatttt ttttcgcaac acgaatgccg gagccccacc gggcactgcc taccagagtc
     3961 cccttcccct gagccgaggc tccctggctg ctgtggccca tgcacagtcc ctggtagaag
     4021 cacagcccaa cgtggacaag ctcgtggagg accacctggc agtgcagtcg ctcatcaggg
     4081 catatcagat acgagggcac catgtagcac agctggaccc cctggggatt ttggatgctg
     4141 atctggactc ctccgtgccc gctgacatta tctcatccac agacaaactt gggttctatg
     4201 gcctggatga gtctgacctc gacaaggtct tccacttgcc caccaccact ttcatcgggg
     4261 gacaggaatc agcacttcct ctgcgggaga tcatccgtcg gctggagatg gcctactgcc
     4321 agcatattgg ggtggagttc atgttcatca atgacctgga gcagtgccag tggatccggc
     4381 agaagtttga gacccctggg atcatgcagt tcacaaatga ggagaaacgg accctgctgg
     4441 ccaggcttgt gcggtccacc aggtttgagg agttcctaca gcggaagtgg tcctctgaga
     4501 agcgctttgg tctagaaggc tgcgaggtac tgatccctgc cctcaagacc atcattgaca
     4561 agtctagtga gaatggcgtg gactacgtga tcatgggcat gccacacaga gggcggctga
     4621 acgtgcttgc aaatgtcatc aggaaggagc tggaacagat cttctgtcaa ttcgattcaa
     4681 agctggaggc agctgatgag ggctccggag atgtgaagta ccacctgggc atgtatcacc
     4741 gcaggatcaa tcgtgtcacc gacaggaaca ttaccttgtc cttggtggcc aacccttccc
     4801 accttgaggc cgctgacccc gtggtgatgg gcaagaccaa agccgaacag ttttactgtg
     4861 gcgacactga agggaaaaag gtcatgtcca tcctgttgca tggggatgct gcatttgctg
     4921 gccagggcat tgtgtacgag accttccacc tcagcgacct gccatcctac acaactcatg
     4981 gcaccgtgca cgtggtcgtc aacaaccaga tcggcttcac caccgaccct cggatggccc
     5041 gctcctcccc ctaccccact gacgtggccc gagtggtgaa tgcccccatt ttccacgtga
     5101 actcagatga ccccgaggct gtcatgtacg tgtgcaaagt ggcggccgag tggaggagca
     5161 ccttccacaa ggacgtggtt gtcgatttgg tgtgttaccg gcgcaacggc cacaacgaga
     5221 tggatgagcc catgttcacg cagccgctca tgtacaagca gatccgcaag cagaagcctg
     5281 tgttacagaa gtacgctgag ctgctggtgt cgcagggtgt ggtcaaccag cctgaatacg
     5341 aagaagagat ttccaagtat gataagatct gtgaggaagc ttttgccaga tctaaagatg
     5401 agaagatctt gcacattaag cactggctgg actctccctg gcctggcttc ttcaccctgg
     5461 acgggcagcc caggagcatg tcctgcccct ccacgggtct gacggaggat attctgacac
     5521 acatcgggaa tgtggctagt tctgtgcctg tggaaaactt tactattcat ggagggctga
     5581 gccggatctt gaagactcgt ggggaaatgg tgaagaaccg gactgtggac tgggctctag
     5641 cggagtacat ggcgtttggc tcgctcctga aggagggcat ccacattcgg ctgagcggcc
     5701 aggacgtgga gcggggcaca ttcagccacc gccaccatgt gctccatgac cagaatgtgg
     5761 acaagagaac ctgcatcccc atgaaccatc tctggcccaa tcaggccccc tatactgtgt
     5821 gcaacagctc actgtctgag tacggcgtgc tgggctttga gctgggcttc gccatggcca
     5881 gtcctaatgc cctggtcctc tgggaagccc aatttggtga cttccacaac acggcccagt
     5941 gtatcatcga ccagttcatc tgcccgggac aagccaagtg ggtgcggcag aatggcatcg
     6001 tgttgctgct gccccatggc atggagggca tgggtccaga acattcctcc gcccgcccag
     6061 agcggttctt gcagatgtgc aacgatgacc cagatgtcct gccagacctt aaagaggcaa
     6121 attttgatat caatcagcta tatgactgca attgggttgt tgtcaactgc tccactcctg
     6181 gcaacttctt ccacgtgcta cgacgccaga tcctgctgcc attccggaag ccgttaatta
     6241 tcttcacccc caaatccctg ttgcgccacc ccgaggccag atccagcttt gatgagatgc
     6301 ttccaggaac ccacttccag cgggtgatcc cagaagatgg ccctgcagct cagaacccag
     6361 aaaatgtcaa aaggcttctc ttctgcaccg gcaaagtgta ttatgacctc acccgggagc
     6421 gcaaagcacg cgacatggtg gggcaggtgg ccatcacaag gattgagcag ctgtcgccat
     6481 tcccctttga cctcctgctg aaggaggtgc agaagtaccc caatgctgag ctggcctggt
     6541 gccaggagga gcacaagaac caaggctact atgactatgt aaaaccgagg cttcggacca
     6601 ccatcagccg cgccaagccc gtctggtatg ccggccggga cccagcggct gctccagcca
     6661 ccggcaacaa gaagacccac ctgacggagc tgcagcgcct cctggacacg gccttcgacc
     6721 tggacgtctt caagaacttc tcgtagccca actttcttgt acaaagtggt tgatatccag
     6781 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     6841 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gatcgacaat caacctctgg
     6901 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6961 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7021 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7081 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7141 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7201 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7261 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     7321 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7381 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7441 agacgagtcg gatctccctt tgggccgcct ccccgcctgg cgatggtacc ggtgtggaaa
     7501 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7561 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7621 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7681 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7741 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7801 tagcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7861 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7921 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     7981 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     8041 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     8101 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     8161 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     8221 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     8281 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacaattcga gctcggtacc
     8341 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8401 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8641 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8701 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8821 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     8881 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8941 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9001 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     9061 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     9121 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     9181 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     9241 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9301 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9361 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9421 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9481 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9541 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9601 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     9661 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     9841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    10081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10501 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    10621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    10681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10801 agaccccg
//
