LOCUS       sequence-214086-        8991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hA3B-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-hA3B-BE3
FEATURES             Location/Qualifiers
     source          1..8991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1603..5001,5002..5004,5005..5601,5602,5603..5605,
                     5606..5607,5608..5610,5611..5703)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1603..1626,1627..1629,1630..5703)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5716..5964
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5977..5997
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6006..6023
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6046..6063)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6052..6276
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6347..6363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6347..6363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6360..6382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6371..6387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6395..6401,6402..6419,6420..6425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6440..6461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6578..6595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6749..7337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6829..6848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7508..8299,8300..8368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8131..8150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8369..8473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8552..8571)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8743..8991,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaatccacag
      421 atcagaaatc cgatggagcg gatgtatcga gacacattct acgacaactt tgaaaacgaa
      481 cccatcctct atggtcggag ctacacttgg ctgtgctatg aagtgaaaat aaagaggggc
      541 cgctcaaatc tcctttggga cacaggggtc tttcgaggcc aggtgtattt caagcctcag
      601 taccacgcag aaatgtgctt cctctcttgg ttctgtggca accagctgcc tgcttacaag
      661 tgtttccaga tcacctggtt tgtatcctgg accccctgcc cggactgtgt ggcgaagctg
      721 gccgaattcc tgtctgagca ccccaatgtc accctgacca tctccgccgc ccgcctctac
      781 tactactggg aaagagatta ccgaagggcg ctctgcaggc tgagtcaggc aggagcccgc
      841 gtgaagatca tggactatga agaatttgca tactgctggg aaaactttgt gtacaatgaa
      901 ggtcagcaat tcatgccttg gtacaaattc gatgaaaatt atgcattcct gcaccgcacg
      961 ctaaaggaga ttctcagata cctgatggat ccagacacat tcactttcaa ctttaataat
     1021 gaccctttgg tccttcgacg gcgccagacc tacttgtgct atgaggtgga gcgcctggac
     1081 aatggcacct gggtcctgat ggaccagcac atgggctttc tatgcaacga ggctaagaat
     1141 cttctctgtg gcttttacgg ccgccatgcg gagctgcgct tcttggacct ggttccttct
     1201 ttgcagttgg acccggccca gatctacagg gtcacttggt tcatctcctg gagcccctgc
     1261 ttctcctggg gctgtgccgg ggaagtgcgt gcgttccttc aggagaacac acacgtgaga
     1321 ctgcgcatct tcgctgcccg catctatgat tacgaccccc tatataagga ggcgctgcaa
     1381 atgctgcggg atgctggggc ccaagtctcc atcatgacct acgatgagtt tgagtactgc
     1441 tgggacacct ttgtgtaccg ccagggatgt cccttccagc cctgggatgg actagaggag
     1501 cacagccaag ccctgagtgg gaggctgcgg gccattctcc agaatcaggg aaacagcggc
     1561 agcgagactc ccgggacctc agagtccgcc acacccgaaa gtgataaaaa gtattctatt
     1621 ggtttagcca tcggcactaa ttccgttgga tgggctgtca taaccgatga atacaaagta
     1681 ccttcaaaga aatttaaggt gttggggaac acagaccgtc attcgattaa aaagaatctt
     1741 atcggtgccc tcctattcga tagtggcgaa acggcagagg cgactcgcct gaaacgaacc
     1801 gctcggagaa ggtatacacg tcgcaagaac cgaatatgtt acttacaaga aatttttagc
     1861 aatgagatgg ccaaagttga cgattctttc tttcaccgtt tggaagagtc cttccttgtc
     1921 gaagaggaca agaaacatga acggcacccc atctttggaa acatagtaga tgaggtggca
     1981 tatcatgaaa agtacccaac gatttatcac ctcagaaaaa agctagttga ctcaactgat
     2041 aaagcggacc tgaggttaat ctacttggct cttgcccata tgataaagtt ccgtgggcac
     2101 tttctcattg agggtgatct aaatccggac aactcggatg tcgacaaact gttcatccag
     2161 ttagtacaaa cctataatca gttgtttgaa gagaacccta taaatgcaag tggcgtggat
     2221 gcgaaggcta ttcttagcgc ccgcctctct aaatcccgac ggctagaaaa cctgatcgca
     2281 caattacccg gagagaagaa aaatgggttg ttcggtaacc ttatagcgct ctcactaggc
     2341 ctgacaccaa attttaagtc gaacttcgac ttagctgaag atgccaaatt gcagcttagt
     2401 aaggacacgt acgatgacga tctcgacaat ctactggcac aaattggaga tcagtatgcg
     2461 gacttatttt tggctgccaa aaaccttagc gatgcaatcc tcctatctga catactgaga
     2521 gttaatactg agattaccaa ggcgccgtta tccgcttcaa tgatcaaaag gtacgatgaa
     2581 catcaccaag acttgacact tctcaaggcc ctagtccgtc agcaactgcc tgagaaatat
     2641 aaggaaatat tctttgatca gtcgaaaaac gggtacgcag gttatattga cggcggagcg
     2701 agtcaagagg aattctacaa gtttatcaaa cccatattag agaagatgga tgggacggaa
     2761 gagttgcttg taaaactcaa tcgcgaagat ctactgcgaa agcagcggac tttcgacaac
     2821 ggtagcattc cacatcaaat ccacttaggc gaattgcatg ctatacttag aaggcaggag
     2881 gatttttatc cgttcctcaa agacaatcgt gaaaagattg agaaaatcct aacctttcgc
     2941 ataccttact atgtgggacc cctggcccga gggaactctc ggttcgcatg gatgacaaga
     3001 aagtccgaag aaacgattac tccatggaat tttgaggaag ttgtcgataa aggtgcgtca
     3061 gctcaatcgt tcatcgagag gatgaccaac tttgacaaga atttaccgaa cgaaaaagta
     3121 ttgcctaagc acagtttact ttacgagtat ttcacagtgt acaatgaact cacgaaagtt
     3181 aagtatgtca ctgagggcat gcgtaaaccc gcctttctaa gcggagaaca gaagaaagca
     3241 atagtagatc tgttattcaa gaccaaccgc aaagtgacag ttaagcaatt gaaagaggac
     3301 tactttaaga aaattgaatg cttcgattct gtcgagatct ccggggtaga agatcgattt
     3361 aatgcgtcac ttggtacgta tcatgacctc ctaaagataa ttaaagataa ggacttcctg
     3421 gataacgaag agaatgaaga tatcttagaa gatatagtgt tgactcttac cctctttgaa
     3481 gatcgggaaa tgattgagga aagactaaaa acatacgctc acctgttcga cgataaggtt
     3541 atgaaacagt taaagaggcg tcgctatacg ggctggggac gattgtcgcg gaaacttatc
     3601 aacgggataa gagacaagca aagtggtaaa actattctcg attttctaaa gagcgacggc
     3661 ttcgccaata ggaactttat gcagctgatc catgatgact ctttaacctt caaagaggat
     3721 atacaaaagg cacaggtttc cggacaaggg gactcattgc acgaacatat tgcgaatctt
     3781 gctggttcgc cagccatcaa aaagggcata ctccagacag tcaaagtagt ggatgagcta
     3841 gttaaggtca tgggacgtca caaaccggaa aacattgtaa tcgagatggc acgcgaaaat
     3901 caaacgactc agaaggggca aaaaaacagt cgagagcgga tgaagagaat agaagagggt
     3961 attaaagaac tgggcagcca gatcttaaag gagcatcctg tggaaaatac ccaattgcag
     4021 aacgagaaac tttacctcta ttacctacaa aatggaaggg acatgtatgt tgatcaggaa
     4081 ctggacataa accgtttatc tgattacgac gtcgatcaca ttgtacccca atcctttttg
     4141 aaggacgatt caatcgacaa taaagtgctt acacgctcgg ataagaaccg agggaaaagt
     4201 gacaatgttc caagcgagga agtcgtaaag aaaatgaaga actattggcg gcagctccta
     4261 aatgcgaaac tgataacgca aagaaagttc gataacttaa ctaaagctga gaggggtggc
     4321 ttgtctgaac ttgacaaggc cggatttatt aaacgtcagc tcgtggaaac ccgccaaatc
     4381 acaaagcatg ttgcacagat actagattcc cgaatgaata cgaaatacga cgagaacgat
     4441 aagctgattc gggaagtcaa agtaatcact ttaaagtcaa aattggtgtc ggacttcaga
     4501 aaggattttc aattctataa agttagggag ataaataact accaccatgc gcacgacgct
     4561 tatcttaatg ccgtcgtagg gaccgcactc attaagaaat acccgaagct agaaagtgag
     4621 tttgtgtatg gtgattacaa agtttatgac gtccgtaaga tgatcgcgaa aagcgaacag
     4681 gagataggca aggctacagc caaatacttc ttttattcta acattatgaa tttctttaag
     4741 acggaaatca ctctggcaaa cggagagata cgcaaacgac ctttaattga aaccaatggg
     4801 gagacaggtg aaatcgtatg ggataagggc cgggacttcg cgacggtgag aaaagttttg
     4861 tccatgcccc aagtcaacat agtaaagaaa actgaggtgc agaccggagg gttttcaaag
     4921 gaatcgattc ttccaaaaag gaatagtgat aagctcatcg ctcgtaaaaa ggactgggac
     4981 ccgaaaaagt acggtggctt cgatagccct acagttgcct attctgtcct agtagtggca
     5041 aaagttgaga agggaaaatc caagaaactg aagtcagtca aagaattatt ggggataacg
     5101 attatggagc gctcgtcttt tgaaaagaac cccatcgact tccttgaggc gaaaggttac
     5161 aaggaagtaa aaaaggatct cataattaaa ctaccaaagt atagtctgtt tgagttagaa
     5221 aatggccgaa aacggatgtt ggctagcgcc ggagagcttc aaaaggggaa cgaactcgca
     5281 ctaccgtcta aatacgtgaa tttcctgtat ttagcgtccc attacgagaa gttgaaaggt
     5341 tcacctgaag ataacgaaca gaagcaactt tttgttgagc agcacaaaca ttatctcgac
     5401 gaaatcatag agcaaatttc ggaattcagt aagagagtca tcctagctga tgccaatctg
     5461 gacaaagtat taagcgcata caacaagcac agggataaac ccatacgtga gcaggcggaa
     5521 aatattatcc atttgtttac tcttaccaac ctcggcgctc cagccgcatt caagtatttt
     5581 gacacaacga tagatcgcaa acgatacact tctaccaagg aggtgctaga cgcgacactg
     5641 attcaccaat ccatcacggg attatatgaa actcggatag atttgtcaca gcttgggggt
     5701 gactctggtg gttctactaa tctgtcagat attattgaaa aggagaccgg taagcaactg
     5761 gttatccagg aatccatcct catgctccca gaggaggtgg aagaagtcat tgggaacaag
     5821 ccggaaagcg atatactcgt gcacaccgcc tacgacgaga gcaccgacga gaatgtcatg
     5881 cttctgacta gcgacgcccc tgaatacaag ccttgggctc tggtcataca ggatagcaac
     5941 ggtgagaaca agattaagat gctctctggt ggttctccca agaagaagag gaaagtctaa
     6001 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     6061 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6121 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6181 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6241 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6301 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6361 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6421 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6481 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6541 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7801 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8101 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8521 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     8581 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     8641 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     8701 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     8761 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     8821 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     8881 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     8941 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat c
//
