LOCUS       sequence-214093-        8553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hA1-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-hA1-BE3
FEATURES             Location/Qualifiers
     source          1..8553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1165..4563,4564..4566,4567..5163,5164,5165..5167,
                     5168..5169,5170..5172,5173..5265)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1165..1188,1189..1191,1192..5265)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5278..5526
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5539..5559
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5568..5585
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5608..5625)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5614..5838
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5909..5925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5909..5925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5922..5944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5933..5949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5957..5963,5964..5981,5982..5987))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6002..6023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6140..6157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6311..6899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6391..6410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7070..7861,7862..7930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7693..7712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7931..8035)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8114..8133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8305..8553,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gacttctgag
      421 aaaggtcctt caaccggtga ccccactctg aggagaagaa tcgaaccctg ggagtttgac
      481 gtcttctatg accccagaga acttcgtaaa gaggcctgtc tgctctacga aatcaagtgg
      541 ggcatgagcc ggaagatctg gcgaagctca ggcaaaaaca ccaccaatca cgtggaagtt
      601 aattttataa aaaaatttac gtcagaaaga gattttcacc catccatgag ctgctccatc
      661 acctggttct tgtcctggag tccctgctgg gaatgctccc aggctattag agagtttctg
      721 agtcggcacc ctggtgtgac tctagtgatc tacgtagctc ggcttttttg gcacatggat
      781 caacaaaatc ggcaaggtct cagggacctt gttaacagtg gagtaactat tcagattatg
      841 agagcatcag agtattatca ctgctggagg aattttgtca actacccacc tggggatgaa
      901 gctcactggc cacaataccc acctctgtgg atgatgttgt acgcactgga gctgcactgc
      961 ataattctaa gtcttccacc ctgtttaaag atttcaagaa gatggcaaaa tcatcttaca
     1021 tttttcagac ttcatcttca aaactgccat taccaaacga ttccgccaca catcctttta
     1081 gctacagggc tgatacatcc ttctgtggct tggagaagcg gcagcgagac tcccgggacc
     1141 tcagagtccg ccacacccga aagtgataaa aagtattcta ttggtttagc catcggcact
     1201 aattccgttg gatgggctgt cataaccgat gaatacaaag taccttcaaa gaaatttaag
     1261 gtgttgggga acacagaccg tcattcgatt aaaaagaatc ttatcggtgc cctcctattc
     1321 gatagtggcg aaacggcaga ggcgactcgc ctgaaacgaa ccgctcggag aaggtataca
     1381 cgtcgcaaga accgaatatg ttacttacaa gaaattttta gcaatgagat ggccaaagtt
     1441 gacgattctt tctttcaccg tttggaagag tccttccttg tcgaagagga caagaaacat
     1501 gaacggcacc ccatctttgg aaacatagta gatgaggtgg catatcatga aaagtaccca
     1561 acgatttatc acctcagaaa aaagctagtt gactcaactg ataaagcgga cctgaggtta
     1621 atctacttgg ctcttgccca tatgataaag ttccgtgggc actttctcat tgagggtgat
     1681 ctaaatccgg acaactcgga tgtcgacaaa ctgttcatcc agttagtaca aacctataat
     1741 cagttgtttg aagagaaccc tataaatgca agtggcgtgg atgcgaaggc tattcttagc
     1801 gcccgcctct ctaaatcccg acggctagaa aacctgatcg cacaattacc cggagagaag
     1861 aaaaatgggt tgttcggtaa ccttatagcg ctctcactag gcctgacacc aaattttaag
     1921 tcgaacttcg acttagctga agatgccaaa ttgcagctta gtaaggacac gtacgatgac
     1981 gatctcgaca atctactggc acaaattgga gatcagtatg cggacttatt tttggctgcc
     2041 aaaaacctta gcgatgcaat cctcctatct gacatactga gagttaatac tgagattacc
     2101 aaggcgccgt tatccgcttc aatgatcaaa aggtacgatg aacatcacca agacttgaca
     2161 cttctcaagg ccctagtccg tcagcaactg cctgagaaat ataaggaaat attctttgat
     2221 cagtcgaaaa acgggtacgc aggttatatt gacggcggag cgagtcaaga ggaattctac
     2281 aagtttatca aacccatatt agagaagatg gatgggacgg aagagttgct tgtaaaactc
     2341 aatcgcgaag atctactgcg aaagcagcgg actttcgaca acggtagcat tccacatcaa
     2401 atccacttag gcgaattgca tgctatactt agaaggcagg aggattttta tccgttcctc
     2461 aaagacaatc gtgaaaagat tgagaaaatc ctaacctttc gcatacctta ctatgtggga
     2521 cccctggccc gagggaactc tcggttcgca tggatgacaa gaaagtccga agaaacgatt
     2581 actccatgga attttgagga agttgtcgat aaaggtgcgt cagctcaatc gttcatcgag
     2641 aggatgacca actttgacaa gaatttaccg aacgaaaaag tattgcctaa gcacagttta
     2701 ctttacgagt atttcacagt gtacaatgaa ctcacgaaag ttaagtatgt cactgagggc
     2761 atgcgtaaac ccgcctttct aagcggagaa cagaagaaag caatagtaga tctgttattc
     2821 aagaccaacc gcaaagtgac agttaagcaa ttgaaagagg actactttaa gaaaattgaa
     2881 tgcttcgatt ctgtcgagat ctccggggta gaagatcgat ttaatgcgtc acttggtacg
     2941 tatcatgacc tcctaaagat aattaaagat aaggacttcc tggataacga agagaatgaa
     3001 gatatcttag aagatatagt gttgactctt accctctttg aagatcggga aatgattgag
     3061 gaaagactaa aaacatacgc tcacctgttc gacgataagg ttatgaaaca gttaaagagg
     3121 cgtcgctata cgggctgggg acgattgtcg cggaaactta tcaacgggat aagagacaag
     3181 caaagtggta aaactattct cgattttcta aagagcgacg gcttcgccaa taggaacttt
     3241 atgcagctga tccatgatga ctctttaacc ttcaaagagg atatacaaaa ggcacaggtt
     3301 tccggacaag gggactcatt gcacgaacat attgcgaatc ttgctggttc gccagccatc
     3361 aaaaagggca tactccagac agtcaaagta gtggatgagc tagttaaggt catgggacgt
     3421 cacaaaccgg aaaacattgt aatcgagatg gcacgcgaaa atcaaacgac tcagaagggg
     3481 caaaaaaaca gtcgagagcg gatgaagaga atagaagagg gtattaaaga actgggcagc
     3541 cagatcttaa aggagcatcc tgtggaaaat acccaattgc agaacgagaa actttacctc
     3601 tattacctac aaaatggaag ggacatgtat gttgatcagg aactggacat aaaccgttta
     3661 tctgattacg acgtcgatca cattgtaccc caatcctttt tgaaggacga ttcaatcgac
     3721 aataaagtgc ttacacgctc ggataagaac cgagggaaaa gtgacaatgt tccaagcgag
     3781 gaagtcgtaa agaaaatgaa gaactattgg cggcagctcc taaatgcgaa actgataacg
     3841 caaagaaagt tcgataactt aactaaagct gagaggggtg gcttgtctga acttgacaag
     3901 gccggattta ttaaacgtca gctcgtggaa acccgccaaa tcacaaagca tgttgcacag
     3961 atactagatt cccgaatgaa tacgaaatac gacgagaacg ataagctgat tcgggaagtc
     4021 aaagtaatca ctttaaagtc aaaattggtg tcggacttca gaaaggattt tcaattctat
     4081 aaagttaggg agataaataa ctaccaccat gcgcacgacg cttatcttaa tgccgtcgta
     4141 gggaccgcac tcattaagaa atacccgaag ctagaaagtg agtttgtgta tggtgattac
     4201 aaagtttatg acgtccgtaa gatgatcgcg aaaagcgaac aggagatagg caaggctaca
     4261 gccaaatact tcttttattc taacattatg aatttcttta agacggaaat cactctggca
     4321 aacggagaga tacgcaaacg acctttaatt gaaaccaatg gggagacagg tgaaatcgta
     4381 tgggataagg gccgggactt cgcgacggtg agaaaagttt tgtccatgcc ccaagtcaac
     4441 atagtaaaga aaactgaggt gcagaccgga gggttttcaa aggaatcgat tcttccaaaa
     4501 aggaatagtg ataagctcat cgctcgtaaa aaggactggg acccgaaaaa gtacggtggc
     4561 ttcgatagcc ctacagttgc ctattctgtc ctagtagtgg caaaagttga gaagggaaaa
     4621 tccaagaaac tgaagtcagt caaagaatta ttggggataa cgattatgga gcgctcgtct
     4681 tttgaaaaga accccatcga cttccttgag gcgaaaggtt acaaggaagt aaaaaaggat
     4741 ctcataatta aactaccaaa gtatagtctg tttgagttag aaaatggccg aaaacggatg
     4801 ttggctagcg ccggagagct tcaaaagggg aacgaactcg cactaccgtc taaatacgtg
     4861 aatttcctgt atttagcgtc ccattacgag aagttgaaag gttcacctga agataacgaa
     4921 cagaagcaac tttttgttga gcagcacaaa cattatctcg acgaaatcat agagcaaatt
     4981 tcggaattca gtaagagagt catcctagct gatgccaatc tggacaaagt attaagcgca
     5041 tacaacaagc acagggataa acccatacgt gagcaggcgg aaaatattat ccatttgttt
     5101 actcttacca acctcggcgc tccagccgca ttcaagtatt ttgacacaac gatagatcgc
     5161 aaacgataca cttctaccaa ggaggtgcta gacgcgacac tgattcacca atccatcacg
     5221 ggattatatg aaactcggat agatttgtca cagcttgggg gtgactctgg tggttctact
     5281 aatctgtcag atattattga aaaggagacc ggtaagcaac tggttatcca ggaatccatc
     5341 ctcatgctcc cagaggaggt ggaagaagtc attgggaaca agccggaaag cgatatactc
     5401 gtgcacaccg cctacgacga gagcaccgac gagaatgtca tgcttctgac tagcgacgcc
     5461 cctgaataca agccttgggc tctggtcata caggatagca acggtgagaa caagattaag
     5521 atgctctctg gtggttctcc caagaagaag aggaaagtct aaccggtcat catcaccatc
     5581 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5641 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5701 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5761 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5821 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga
     5881 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5941 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct
     6001 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6061 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6121 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6181 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6241 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6301 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6361 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6421 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6481 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6541 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6601 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6661 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6721 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6781 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6841 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6901 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6961 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7021 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7081 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7141 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7201 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7261 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7321 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7381 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7441 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7501 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7561 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7621 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7681 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7741 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7801 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7861 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7921 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7981 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8041 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag
     8101 ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8161 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8221 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8281 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     8341 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     8401 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     8461 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     8521 aaactgccca cttggcagta catcaagtgt atc
//
