LOCUS       sequence-214109-        8142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET28-derived plasmid that expresses an N-terminal maltose binding 
            fusion construct of CreM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Waldman AJ, Balskus EP
  TITLE     Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.
  JOURNAL   J Org Chem. 2018 May 29. doi: 10.1021/acs.joc.8b00367.
  PUBMED    29771512
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Org Chem.
            2018 May 29. doi: 10.1021/acs.joc.8b00367."
COMMENT     Sequence Label: pET-MBP-creM
FEATURES             Location/Qualifiers
     source          1..8142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      29..617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     518..537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     771..788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    803..942
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(868..887)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1028..1050
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1044..1235)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2044..3126)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3088..3107
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3127..3204)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3410..3429
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3513..3532
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3513..3531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3532..3556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3571..3593
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3612..3629
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3639..3656
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3660..4760
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      4617..4626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4734..4757
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4773..4790
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4806..4823
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             4824..4844
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             6519..6536
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6589..6607)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6603..6650
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6687..7142
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6774..6793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6984..7005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7234..8049)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7234..8049)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7957..7976
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8041..8060
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
       61 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
      181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      661 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      781 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      841 tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      901 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
      961 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     1021 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     1081 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     1141 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     1201 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     1261 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     1321 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     1381 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     1441 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     1501 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     1561 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     1621 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     1681 agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg
     1741 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     1801 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     1861 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     1921 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     1981 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
     2041 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag
     2161 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg
     2221 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat
     2281 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg
     2341 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag
     2401 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat
     2461 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt
     2521 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc
     2581 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg
     2641 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac
     2701 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat
     2761 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc
     2821 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat
     2881 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag
     2941 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc
     3001 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac
     3061 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg
     3121 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa
     3181 ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca
     3241 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
     3301 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     3361 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     3421 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     3481 cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg
     3541 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacca
     3601 tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata
     3661 tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc
     3721 tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc
     3781 cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta
     3841 tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca
     3901 ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca
     3961 acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag
     4021 atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga
     4081 aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc
     4141 tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag
     4201 acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta
     4261 aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag
     4321 gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag
     4381 tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg
     4441 gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggcg aaagagttcc
     4501 tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg
     4561 gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca
     4621 ccatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct
     4681 ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag
     4741 ccctgaaaga cgcgcagact aattcgagct cccaccatca ccatcaccac gcgaattcgg
     4801 taccgctggt tccgcgtgga tctgagaacc tgtacttcca atccggatcc gtgcacgcac
     4861 tgctgcgccg ggtcacacgc gggaacggct tctacatcgg cgacctcttc cacgccgccg
     4921 ccggccacga cgccaccaca ccggtcacgc tcgaccagcc ccttcagtac gcccccgagc
     4981 tcggcacgcg cttcaccgtc gggcagctcg ccgagcagac ggacgaactg gccgcccggc
     5041 tctgggccgc gggcgtgcgg cccaccgaac gggtcgcgct gtacaagaag gacaacttcg
     5101 acatcgcgct gcacgcctgc gccgccgcgc ggatcggcgc ggtccccgcc ctcctgtcgc
     5161 ccgccctgga ggccccggtc gtacggacgc tgctcgaccg gctcgacggg ccgtggctgc
     5221 tgaccgacgc gacgaccctc gccggccccc tggggacggt gctcgcaccg gcatccgtac
     5281 gagcggtcct gctgacgacc ggcacaccgg cggagggcgc gcttcccgca gccggcgcga
     5341 ccggacccgc tcgcgaaggg gacgcgccgc cgcccgctcc cgtcgtgcgg ctggccgacc
     5401 accggggcgc cccgaagcgg ccgcccgtct tcctgcaccc gcgccagccc tcgctgatca
     5461 cgcacagctc gggaaccacc ggcgtcccca agctggcggt gcactgcccc gaggcgggct
     5521 ggcaccggct ggttccgcag aaggtcgtgt cctggccgat ccgcggcaag gagaaggcgg
     5581 cgctgtgcat gacgttcgtg cactcgcgct tctaccaggg cctggccatg ttcctcagcc
     5641 acggcaaccc gatgctgatc gccgtcgacc ccgacccgag ccggatcggc ccgctgttcg
     5701 cgcgcgaacg gcccggctac atcgagaccc accccaacac ctacgtcgac tgggaagcgc
     5761 tggccgacgc cccgggcgaa ccgctcgccg gcgtacgggt cttcggtgcg accttcgacg
     5821 cgatgcaccc ccggaccatc cagcgcatgc tcggcgcctc ccggcgcacc cgcccgctgt
     5881 tcgtccagtt ctacggccag tcggagatcg gcccgatggc cggccgctgg tacacccggc
     5941 gctcggcggc caggatgaac ggccgctgcg tggggctgcc gctgcccggc ttcatctcgc
     6001 tgcgggtggt cgacgacgcc gggaagcggc tgcgcggcgg gcgcaccggc cacctcgaag
     6061 tgcgcagcag gacgcgcatc ctcacctacc tcggcgagga ccagcggtac gcggagcaat
     6121 tgcacgacgg atggtggcgg gtgggcgaca tgggccgccg cgaccgctgg ggcctgctgc
     6181 acctgctgga ccgcgaggtc gaccggatcg gcgacctgga gagcagcctg gcggtggagg
     6241 acctgctgat gagccgcctg gaggaactgc gcgaggtggt cctggtgccc ggggcggacg
     6301 gcgaaccggt ccccgtcgtc gccaccgtcg acgagagccc gctcgacgcg gcgcgctggc
     6361 agcgggcgac cagcgacctg ccgacgatgg cgccggtgcg ccagttccgg ttcgaagacc
     6421 tgccgcgcac gtccacccgc aagatccaga aaccggaact ggcccggctg atccagggcg
     6481 tccgggcaac cgaccaggga gtgggggcat gactcgagca ccaccaccac caccactgag
     6541 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     6601 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     6661 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6721 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6781 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6841 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6901 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6961 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7021 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7081 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7141 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7201 cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg
     7261 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     7321 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     7381 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     7441 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     7501 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     7561 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     7621 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     7681 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     7741 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     7801 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     7861 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     7921 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     7981 taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat
     8041 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     8101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc ag
//
