LOCUS       sequence-21413-e         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, 
            Cantley LC
  TITLE     The tumor suppressor LKB1 kinase directly activates AMP-activated 
            kinase and regulates apoptosis in response to energy stress.
  JOURNAL   Proc Natl Acad Sci U S A 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 
            25.
  PUBMED    14985505
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2004-03-9"; volume: "101(10)"; pages: 
            "3329-35. Epub 2004 Feb 25"
COMMENT     Sequence Label: pcDNA4-STRAD alpha
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..168
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             181..198
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             202..234
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(238..246,247..261)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcttagcgc agaggcttgg ggcagccgag cggcagccag gccccggccc gggcctcggt
       61 tccagaaggg agaggagccc gccaaggcgc gcaagagagc gggctgcctc gcagtccgag
      121 ccggagaggg agcgcgagcc gcgccggccc cggacggcct ccgaaaccat ggggggttct
      181 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
      241 ctgtacgacg atgacgataa ggtaccagga tccagtgtgg tggaattcct catgtcattt
      301 cttaccaatg atgcgagctc agagtcaata gcatccttct ctaaacagga ggtcatgagt
      361 agctttctgc cagagggagg gtgttacgag ctgctcactg tgataggcaa aggatttgag
      421 gacctgatga ctgtgaatct agcaaggtac aaaccaacag gagagtacgt gactgtacgg
      481 aggattaacc tagaagcttg ttccaatgag atggtaacat tcttgcaggg cgagctgcat
      541 gtctccaaac tcttcaacca tcccaatatc gtgccatatc gagccacttt tattgcagac
      601 aatgagctgt gggttgtcac atcattcatg gcatacggtt c
//
