LOCUS       sequence-21418-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz 
            L, Nusse R, Weissman IL
  TITLE     A role for Wnt signalling in self-renewal of haematopoietic stem 
            cells.
  JOURNAL   Nature. 2003 May 22. 423(6938):409-14.
  PUBMED    12717450
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 22. 423(6938):409-14."
COMMENT     Sequence Label: MSCV-beta-catenin-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             82..117
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaattg gccaccatgg ctactcaagc tgacctgatg
       61 gagttggaca tggccatgga gccggacaga aaagctgctg tcagccactg gcagcagcag
      121 tcttacttgg atgctggaat ccatgctggt gccaccgcca cagctcctgc cctgagtggc
      181 aagggcaacc ctgaggaaga agatgttgac acctcccaag tcctttatga atgggagcaa
      241 ggcttttccc agtccttcac gcaagagcaa gtagctgata ttgacgggca gtatgcaatg
      301 actagggctc agagggtccg agctgccatg ttccctgaga cgctagatga gggcatgcag
      361 atcccatcca cgcagtttga cgctgctcat cccactaatg tccagcgctt ggctgaacca
      421 tcacagatgt tgaaacatgc agttgtcaat ttgattaact atcaggatga cgcggaactt
      481 gccacacgtg caattcctga gctgacaaaa ctgctaaacg atgaggacca ggtggtagtt
      541 aataaagctg ctgttatggt ccatcagctt tccaaaaagg aagcttccag acatgccatc
      601 atgcgctccc ctcagatggt gtctgccatt gtacgcacca tgcagaatac aaatgatgta
      661 gagacagctc gttgtactgc tgggactctg cacaaccttt ctcaccaccg cgagggcttg
      721 ctggccatct
//
