LOCUS       sequence-214184-       12457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive 
            mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 12457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry
FEATURES             Location/Qualifiers
     source          1..12457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1612..1665
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1672..1674,1675..2388)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1672..1674,1675..2385)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1714..1735)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1975..1994)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2322..2343
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2511..3010
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2546..2569)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2943..2962
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3031..3741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3176..3194)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3444..3463
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3818..4406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3871..3891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4289..4300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4409..4425)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4410..4426)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4516..4749
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4774..4792)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4775..4792)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5082..5101)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5315..5334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5434..5456
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5494..5512)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5580..5684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5685..5753,5754..6545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5903..5922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6716..7304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7205..7224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7458..7475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7545..7565)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7550..7879
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7730..7865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9013..9078
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9208..9228
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9653..9787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9690..9709)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9744..9763
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9955..10588
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10635..10760
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11257..11490
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11675..11719
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12004..12121
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     12347..12371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      join(12452..12457,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggcatcg tggagccggg ttgcggagac atgctgacgg gcaccgagcc gatgccgggg
       61 agcgacgagg gccgggcgcc tggcgccgac ccgcagcacc gctacttcta cccggagccg
      121 ggcgcgcagg acgcggacga gcgtcgcggg ggcggcagcc tggggtctcc ctacccgggg
      181 ggcgccttgg tgcccgcccc gccgagccgc ttccttggag cctacgccta cccgccgcga
      241 ccccaggcgg ccggcttccc cggcgcgggc gagtccttcc cgccgcccgc ggacgccgag
      301 ggctaccagc cgggcgaggg ctacgccgcc ccggacccgc gcgccgggct ctacccgggg
      361 ccgcgtgagg actacgcgct acccgcggga ctggaggtgt cggggaaact gagggtcgcg
      421 ctcaacaacc acctgttgtg gtccaagttt aatcagcacc agacagagat gatcatcacc
      481 aagcagggac ggcggatgtt cccattcctg tcatttactg tggccgggct ggagcccacc
      541 agccactaca ggatgtttgt ggacgtggtc ttggtggacc agcaccactg gcggtaccag
      601 agcggcaagt gggtgcagtg tggaaaggcc gagggcagca tgccaggaaa ccgcctgtac
      661 gtccacccgg actcccccaa cacaggagcg cactggatgc gccaggaagt ttcatttggg
      721 aaactaaagc tcacaaacaa caagggggcg tccaacaatg tgacccagat gattgtgctc
      781 cagtccctcc ataagtacca gccccggctg catatcgttg aggtgaacga cggagagcca
      841 gaggcagcct gcaacgcttc caacacgcat atctttactt tccaagaaac ccagttcatt
      901 gccgtgactg cctaccagaa tgccgagatt actcagctga aaattgataa taaccccttt
      961 gccaaaggat tccgggagaa ctttgagtcc atgtacacat ctgttgacac cagcatcccc
     1021 tccccgcctg gacccaactg tcaattcctt gggggagatc actactctcc tctcctaccc
     1081 aaccagtatc ctgttcccag ccgcttctac cccgaccttc ctggccaggc gaaggatgtg
     1141 gttccccagg cttactggct gggggccccc cgggaccaca gctatgaggc tgagtttcga
     1201 gcagtcagca tgaagcctgc attcttgccc tctgcccctg ggcccaccat gtcctactac
     1261 cgaggccagg aggtcctggc acctggagct ggctggcctg tggcacccca gtaccctccc
     1321 aagatgggcc cggccagctg gttccgccct atgcggactc tgcccatgga acccggccct
     1381 ggaggctcag agggacgggg accagaggac cagggtcccc ccttggtgtg gactgagatt
     1441 gcccccatcc ggccggaatc cagtgattca ggactgggcg aaggagactc taagaggagg
     1501 cgcgtgtccc cctatccttc cagtggtgac agctcctccc ctgctggggc cccttctcct
     1561 tttgataagg aagctgaagg acagttttat aactattttc ccaacaccgg tgagggcaga
     1621 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gcccttccgg ggtgagcaag
     1681 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1741 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1801 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1861 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1921 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1981 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2041 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2101 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     2161 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2221 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2281 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2341 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaga tccttgactt
     2401 gcggccgcaa ctcccacctg caacatgcgt gactgactga ggccgcgact ctagagtcga
     2461 cctgcatcta ggcgccggaa ttagatctct cgaggttaac gaattctacc gggtagggga
     2521 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     2581 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     2641 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     2701 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     2761 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     2821 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     2881 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     2941 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     3001 gggcctttcg acctgcagcc caagcttacc atggtgagca agggcgagga ggataacatg
     3061 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     3121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     3181 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     3241 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     3301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     3361 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     3421 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     3481 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     3541 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     3601 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     3661 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     3721 atggacgagc tgtacaagta aagcggccgc gactctagag tcgacctgca ggcatgcaag
     3781 cttgcaggca tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat
     3841 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3901 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3961 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4021 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4081 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4141 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4201 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4261 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4321 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4381 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaattcga
     4441 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4501 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4561 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4621 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4681 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4741 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
     4801 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
     4861 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
     4921 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
     4981 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
     5041 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     5101 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     5161 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     5221 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     5281 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5341 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5401 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5461 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5521 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5581 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5641 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5701 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5761 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5821 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5881 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5941 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6001 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6061 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6121 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6181 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6241 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6301 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6361 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6421 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6481 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6541 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6601 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6661 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6721 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6781 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6841 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6901 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6961 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7021 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7081 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7141 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7201 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7261 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7321 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7381 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7441 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7501 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     7561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7801 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7861 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
     7921 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     7981 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     8041 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     8101 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     8161 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
     8221 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     8281 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     8341 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     8401 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     8461 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     8521 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     8581 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     8641 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     8701 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     8761 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     8821 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     8881 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
     8941 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     9001 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     9061 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     9121 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     9181 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     9241 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     9301 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     9361 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     9421 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     9481 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     9541 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     9601 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     9661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9781 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     9841 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     9901 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     9961 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
    10021 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
    10081 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
    10141 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
    10201 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
    10261 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
    10321 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
    10381 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    10441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10501 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10561 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    10621 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    10681 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    10741 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    10801 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    10861 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    10921 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    10981 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    11041 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    11101 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    11161 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    11221 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    11281 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    11341 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    11401 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    11461 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    11521 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    11581 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    11641 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    11701 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    11761 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    11821 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    11881 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    11941 cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca
    12001 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    12061 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    12121 tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt
    12181 cggagcactg tcctccgaac gtcggagcac tgtcctccga acgtcggagc actgtcctcc
    12241 gaacgtcgga gcactgtcct ccgaacggag catgtcctcc gaacgtcgga gcactgtcct
    12301 ccgaacgact agttaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
    12361 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
    12421 accgatccag cctctcgaca ttcgttggat cgccacc
//
