LOCUS       sequence-214190-       11995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Streptomyces/E.coli shuttle expression vector encoding creD, creE, 
            creM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Waldman AJ, Balskus EP
  TITLE     Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.
  JOURNAL   J Org Chem. 2018 May 29. doi: 10.1021/acs.joc.8b00367.
  PUBMED    29771512
REFERENCE   2  (bases 1 to 11995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Org Chem.
            2018 May 29. doi: 10.1021/acs.joc.8b00367."
COMMENT     Sequence Label: pUWL201PW-creDEM
FEATURES             Location/Qualifiers
     source          1..11995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      13..468
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(100..119)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     310..331
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     terminator      complement(692..740)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     primer_bind     complement(4803..4819)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             4839..4850
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(10000..10016)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10086..10103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10257..10845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10337..10356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11016..11807,11808..11876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11639..11658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11877..11981)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
       61 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      121 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      181 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      241 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      301 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      361 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
      421 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
      481 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagag
      541 cttggccgga tctaaagttt tgtcgtcttt ccagacgtta gtaaatgaat tttctgtatg
      601 aggttttgct aaacaacttt caacagtttc agcggagtga gaatagaaag gaacaactaa
      661 aggaattgcg aataataatt ttttcacgtt gaaaatctcc aaaaaaaaag gctccaaaag
      721 gagcctttaa ttgtatcggt ttatcagctt gctttcgagg tgaatttctt aaacagcttg
      781 ataccgatag ttgcgccgac aatgacaaca accatcgccc acgcataacc gatatattcg
      841 gtcgctgagg cttgcaggga gtcaaaggcc gcttttgcgg gatcatcact gacgaatcga
      901 ggtcgaggaa ccgagcgtcc gaggaacaga ggcgcttatc ggttggccgc gagattcctg
      961 tcgatcctct cgtgcagcgc gattccgagg gaaacggaaa cgttgagaga ctcggtctgg
     1021 ctcatcatgg ggatggaaac cgaggcggaa gacgcctcct cgaacaggtc ggaaggccca
     1081 cccttttcgc tgccgaacag caaggccagc cgatccggat tgtccccgag ttccttcacg
     1141 gaaatgtcgc catccgcctt gagcgtcatc agctgcatac cgctgtcccg aatgaaggcg
     1201 atggcctcct cgcgaccgga gagaacgacg ggaagggaga agacgtaacc tcggctggcc
     1261 ctttggagac gccggtccgc gatgctggtg atgtcactgt cgaccaggat gatccccgac
     1321 gctccgagcg cgagcgacgt gcgtactatc gcgccgatgt tcccgacgat cttcaccccg
     1381 tcgagaacga cgacgtcccc acgccggctc gcgatatcgc cgaacctggc cgggcgaggg
     1441 acgcgggcga tgccgaatgt cttggccttc cgctccccct tgaacaactg gttgacgatc
     1501 gaggagtcga tgaggcggac cggtatgttc tgccgcccgc acagatccag caactcagat
     1561 ggaaaaggac tgctgtcgct gccgtagacc tcgatgaact ccaccccggc cgcgatgctg
     1621 tgcatgaggg gctcgacgtc ctcgatcaac gttgtcttta tgttggatcg cgacggcttg
     1681 gtgacatcga tgatccgctg caccgcggga tcggacggat ttgcgatggt gtccaactca
     1741 gtcatggtcg tcctaccggc tgctgtgttc agtgacgcga ttcctggggt gtgacaccct
     1801 acgcgacgat ggcggatggc tgccctgacc ggcaatcacc aacgcaaggg gaagtcgtcg
     1861 ctctctggca aagctccccg ctcttccccg tccgggaccc gcgcggtcga tccccgcata
     1921 tatgaagtat tcgccttgat cagtcccggt ggacgcgcca gcggcccgcc ggagcgacgg
     1981 actccccgac ctcgatcgtg tcgccctgag cgtccacgta gacgttgcgt gagagcagga
     2041 ctgggccgcc gccgaccgca ccgccctcac caccgaccgc gaccgcgcca tggccgccgc
     2101 cgacggcctg gtcgccgccg ccgcccgccg gttcggcgcc tgacccgacc aacccccgcg
     2161 gggcgccggc acttcgtgct ggcgccccgc ccccacccac caggagaccg accatgaccg
     2221 acttcgacgg acgcctgacc gaggggaccg tgaacctggt ccaggacccc aacggcggtg
     2281 gctggtccgc ccactgcgct gagcccggtt gcgactgggc cgacttcgcc ggaccgctcg
     2341 gcttccaggg cctcgtggcc atcgctcgcc gacacacgca ctgaccgcac gtcaaagccc
     2401 cgccggatca ccggcggggc tctcttcggc cctccaagtc acaccagccc caaggggcgt
     2461 cgggagtggc ggagggaacc tctggcccga ttggtgccag gattcccacc agaccaaaga
     2521 gcaacgggcc ggacttcgca cctccgaccc gtccgctccc agactcgcgc cccttagccg
     2581 ggcgagacag gaacgttgct cgtgcccaga gtacggagcg atgccgaggc attgccagat
     2641 cggcccgccg ggccccgctg ccactgcggg accgcaattg cccacacacc gggcaaacgg
     2701 ccgcgtatct actgctcaga ccgctgccgg atggcagcga agcgggcgat cgcgcgtgtg
     2761 acgcgagatg ccgcccgagg caaaagcgaa caccttggga aagaaacaac agagtttccc
     2821 gcacccctcc gacctgcggt ttctccggac ggggtggatg gggagagccc gagaggcgac
     2881 agcctctcgg aagtaggaag cacgtcgcgg agcgacgctg cccgactgcg gaaagccgcc
     2941 cggtacagcc gccgccggac gctgtggcgg atcagcgggg acgccgcgtg caagggctgc
     3001 ggccgcgccc tgatggaccc tgcctccggc gtaatcgtcg cccagacggc ggccggaacg
     3061 tccgtggtcc tgggcctgat gcggtgcggg cggatctggc tctgcccggt ctgcgccgcc
     3121 acgatccggc acaagcgggc cgaggagatc accgccgccg tggtcgagtg gatcaagcgc
     3181 ggggggaccg cctacctggt caccttcacg gcccgccatg ggcacacgga ccggctcgcg
     3241 gacctcatgg acgccctcca gggcacccgg aagacgccgg acagcccccg gcggccgggc
     3301 gcctaccagc gactgatcac gggcggcacg tgggccggac gccgggccaa ggacgggcac
     3361 cgggccgccg accgcgaggg catccgagac cggatcgggt acgtcggcat gatccgcgcg
     3421 accgaagtca ccgtggggca gatcaacggc tggcacccgc acatccacgc gatcgtcctg
     3481 gtcggcggcc ggaccgaggg ggagcggtcc gcgaagcaga tcgtcgccac cttcgagccg
     3541 accggcgccg cgctcgacga gtggcagggg cactggcggt ccgtgtggac cgccgccctg
     3601 cgcaaggtca accccgcctt cacgcccgac gaccggcacg gcgtcgactt caagcggctg
     3661 gagaccgagc gcgacgccaa cgacctcgcc gagtacatcg ccaagaccca ggacgggaag
     3721 gcgcccgccc tcgaactcgc ccgcgccgac ctcaagacgg cgaccggcgg gaacgtcgcc
     3781 ccgttcgaac tcctcggacg gatcggggac ctgaccggcg gcatgaccga ggacgacgcc
     3841 gccggggtcg gctcgctgga gtggaacctc tcgcgctggc acgagtacga gcgggcaacc
     3901 cggggacgcc gggccatcga atggacccgc tacctgcggc agatgctcgg gctcgacggc
     3961 ggcgacaccg aggccgacga cctcgatctg ctcctggcgg ccgacgccga cggcggggag
     4021 ctgcgggccg gggtcgccgt gaccgaggac ggatggcacg cggtcacccg ccgcgccctc
     4081 gacctcgagg cgacccgggc cgccgaaggc aaggacggca acgaggattc ggcggccgtg
     4141 ggcgaacggg tgcgggaggt cctggcgctg gccgacgcgg ccgacacagt ggtggtgctc
     4201 acggcggggg aggtggccga ggcgtacgcc gacatgctcg ccgccctcgc ccagcgccgc
     4261 gaggaagcaa ctgcacgccg acggcgagag caggacgacg accaggacga cgacgccgac
     4321 gaccgccagg agcgggccgc ccggcacatc gcccggctcg caagtgggcc cacttcgcac
     4381 taactcgctc ccccccgccg tacgtcatcc cggtgacgta cggcgggggt cggtgacgta
     4441 cgcggcgacg gcggccgggg tcgaagccgc gggagtaatc ctgggattac tcgcccgggg
     4501 tcggccccgc cggcacttcg tgcaggcggt accagcccga cccgagcacg cgccggcacg
     4561 cctggtcgat gtcggaccgg agttcgaggt acgcggcttg caggtccagg aaggggacgt
     4621 ccatgcgagt gtccgttcga gtggcggctt gcgcccgatg ctagtcgcgg ttgatcggcg
     4681 atcgcaggtg cacgcggtcg atcttgacgg ctggcgagag gtgcggggag gatctgaccg
     4741 acgcggtcca cacgtggcac cgcgatgctg ttgtgggcac aatcgtgccg gttggtagga
     4801 tcgatccact agttctagaa ataattttgt ttaacattaa agaggagaaa ttacatatga
     4861 ccagaccccc cgcacccccg cccggcgccc ccggcgccga cgagctgctc gactgcgggc
     4921 tgctgtcccc cgtccgggcc ggcacccccg tcgaagcgct ggtctgcgac tccgcctggc
     4981 tccaggcgat gctcgacgcg gaggccgcgc tgacccgcgc ccaggccagg accggcttcc
     5041 tgcccgccgc cgcggccgag gcgatcaccg cggcggcgcg ggccgaccgg atcgacctgc
     5101 tcgcggtcgc ccggggcgcc cgggagaccg ccaaccccgt ggtggggctg gtggcggcgc
     5161 tcaccgccgc ggtccgccgg gacgacccgg cggcggccga gtacgtccac cggggctcca
     5221 ccagccagga cgtgctggac accggggcga tgctcgtcgc ccgccgggcg ctgcgcctga
     5281 tcggcgacga cctggaccgg gcggccgacg ccctcgcggc gctcgccgcc gaccaccgcg
     5341 acacccccat ggccggccgc acgctggccc tgcacgccgt gccgacgacg ttcgggctga
     5401 aggccgctgg ctggctggaa ctcgtcagcg aggccgcggg gcgggtggcg cggctgcgcg
     5461 acgggctgcc gttctcgctg ggcggcgcgg cgggcacgct cgccgggtac ttcggcgacc
     5521 gcacggaccg gggcgacccg gccgtcctcc tcgaccggct cctcgacgcc tacgcggcgg
     5581 agaccgggct cgcccggccg gtgctgccct ggcacgtgct gcgcaccccc gtggccgatc
     5641 tggccgccgt gctcgcgttc accgccgggg cgctcgggaa gatcgcggtg gacgtgcagt
     5701 cactggcccg cacggaggtg gcggaagtcg ccgagccggc cgtggagggg cgcggcgctt
     5761 cctccgcgat gccgcacaaa cgcaatcccg tcctgtcgac gctgatccgc agcgctgcgc
     5821 tccaagtgcc cgccctggcc acggggttga cccagtgcct ggtgtcggag gacgagcggt
     5881 cggcgggcgc ctggcacgcg gagtggcaac cgctgcgcga gtgcctgcgg ctgaccgggg
     5941 gcgccgcccg caccgccgtg gagctcgcgg cggggctgga ggtggacgcc gcccggatgc
     6001 gggccaacct cgacctcacg gacggccgga tcgtctcgga gtcggtcgcg gtggcgctca
     6061 ccccgctgct cggcaggcag gcggcgaagg aactgctgac ccgggccgcg ttcaccgccg
     6121 gccacgaggg ccggacgctg ggcgaggtgc tcggcgaact gcccgaactg gacggggtgc
     6181 tgccgaagga gcggtgggag gcgctgctcg atccggcacg tgccaccggg gtcgcgggag
     6241 cgctggtgga cggcgcgctc gcccggcgcc gcccgccggc ccggtgacct agcggaggag
     6301 cccatatgag cgtcagacgg ctcaccgtgt gcatcgtcgg tgcgggaccg cgcggactgt
     6361 cggtgctgga aaggttctgt gcccacgaac ggaagtccgc ctcgcacccg gcggtgacgg
     6421 ttcacgtcgt ggacccggcc cgcccgggcg cgggccgggt gtggcggacg ggtcagccgc
     6481 gccagctgct gatgaacacg gtcgcctcgc aggtgaccgt gttcaccgac gggtccgtcg
     6541 acatggcggg cccggtggag gccggcccca gcctccacga gtgggcgcgc gaactggccg
     6601 ccctcacccc cgtggaggag ctgctcggcg gccacgacga cgccaccctg gccgaggccc
     6661 gggccctggg cgccgactcg taccccaccc gggccttcta cgggtgctac ctggaggaga
     6721 tgttccgccg ggtggtctgc ggcgcgcccg cccacctgga ggtgcgggtg caccgctcga
     6781 ccgccgtctc cctggcggac gagacgccgg gctccggagg cgcgcagagc ctgctgctcg
     6841 ccgacggcac ccggctggcc gggctcgacg cggtcgtgct cgcgctcggc cacgtccgcg
     6901 cggaggagcc gggcgccccc gacccgcgcg ccgccgcgct cggcctggcc cacttcccgc
     6961 cggccaaccc cgccgacctc gacctgtccg gcatcgcgcc cggcaccccc gtcctgctgc
     7021 gcgggctcgg cctgaacttc ttcgaccaca tggcgctgtt caccctcggc cggggcggcg
     7081 ccttcagccg ccgcccgcac gggctgcggt accacccctc ggggctcgaa ccgcggctct
     7141 acgccggttc gcggcgcggg gtcccgtacc acgcgcgcgg ggagaacgag aagggcgtgg
     7201 acgggcgcca caccccgctg ctgctcaccc ccgagcgcat cgccgaactg accggccggc
     7261 accgcgaggg ccccggcctg tcgttcctcc ggacgctgtg gcccctgatc gcccgcgagg
     7321 tcgaatgcgt ctactacgga acgctgctcg cctcccgcgg ccgggcggcc gaacgggacg
     7381 ccttcgtgac ggcgtacctg gcgggcggcg acgacacgga ccggggcggg gtgctggaac
     7441 gtttcggcat cggcccggcc gaccggtggt gctgggagcg gaccgcgtcc ccgcaccccc
     7501 ggcacggctt caccgggccc gacgggcacc gccgctggct gctggagcac ctggcccagg
     7561 acgtgcgccg ggccagggcg ggcaatgtga gcgatccgca caaggccgcc ctggacgtgc
     7621 tgcgcgacct gcgcaacgag atccggctcg tggtggacca cggcgggctc gacggcctgt
     7681 cgcaccggga cgacctcgac ggctggtaca ccggcctgaa cgcgttcctc tcgatcggcc
     7741 cgcccgcctc ccgcatcgag gagatggcgg ccctgatcga ggcgggcgtg ctcgacgtcg
     7801 tcggccccgg cctggaggtc gacatcgacg aggcggacgc cgcgttcgtc gcccgctccc
     7861 cgctggtccc cggccggccg gtgcgcgccc acgtgctgat cgaggcccgg ctgccggtga
     7921 cggacctgcg ccgtaccgcc gatccgctgt tgcgcgacct gctgcgcagc gggcagtgcc
     7981 ggtcctaccg catccccgcc ggccgggcgc cggagggcta cgagaccggt ggcctcgaag
     8041 tcacccgtcg cccgtaccgc ctggtggacg cgctgggccg ggcgcacccg cggcgcttcg
     8101 ccttcggcgt gcccacggag gccgtgcact gggtcaccgc cgccggggcg cggcccggcg
     8161 tcaactcggt caccctgggg gacgccgacg cgatagccca cgccgtcgcc tcgctcacgc
     8221 cggccgccgc cccccggctg cccgcgtacg aggaccccgg cgtccgctgc ccctccgacg
     8281 accggctcac cgaggtgacg gcatgaccta gcggaggagc ccatatgcac gcactgctgc
     8341 gccgggtcac acgcgggaac ggcttctaca tcggcgacct cttccacgcc gccgccggcc
     8401 acgacgccac cacaccggtc acgctcgacc agccccttca gtacgccccc gagctcggca
     8461 cgcgcttcac cgtcgggcag ctcgccgagc agacggacga actggccgcc cggctctggg
     8521 ccgcgggcgt gcggcccacc gaacgggtcg cgctgtacaa gaaggacaac ttcgacatcg
     8581 cgctgcacgc ctgcgccgcc gcgcggatcg gcgcggtccc cgccctcctg tcgcccgccc
     8641 tggaggcccc ggtcgtacgg acgctgctcg accggctcga cgggccgtgg ctgctgaccg
     8701 acgcgacgac cctcgccggc cccctgggga cggtgctcgc accggcatcc gtacgagcgg
     8761 tcctgctgac gaccggcaca ccggcggagg gcgcgcttcc cgcagccggc gcgaccggac
     8821 ccgctcgcga aggggacgcg ccgccgcccg ctcccgtcgt gcggctggcc gaccaccggg
     8881 gcgccccgaa gcggccgccc gtcttcctgc acccgcgcca gccctcgctg atcacgcaca
     8941 gctcgggaac caccggcgtc cccaagctgg cggtgcactg ccccgaggcg ggctggcacc
     9001 ggctggttcc gcagaaggtc gtgtcctggc cgatccgcgg caaggagaag gcggcgctgt
     9061 gcatgacgtt cgtgcactcg cgcttctacc agggcctggc catgttcctc agccacggca
     9121 acccgatgct gatcgccgtc gaccccgacc cgagccggat cggcccgctg ttcgcgcgcg
     9181 aacggcccgg ctacatcgag acccacccca acacctacgt cgactgggaa gcgctggccg
     9241 acgccccggg cgaaccgctc gccggcgtac gggtcttcgg tgcgaccttc gacgcgatgc
     9301 acccccggac catccagcgc atgctcggcg cctcccggcg cacccgcccg ctgttcgtcc
     9361 agttctacgg ccagtcggag atcggcccga tggccggccg ctggtacacc cggcgctcgg
     9421 cggccaggat gaacggccgc tgcgtggggc tgccgctgcc cggcttcatc tcgctgcggg
     9481 tggtcgacga cgccgggaag cggctgcgcg gcgggcgcac cggccacctc gaagtgcgca
     9541 gcaggacgcg catcctcacc tacctcggcg aggaccagcg gtacgcggag caattgcacg
     9601 acggatggtg gcgggtgggc gacatgggcc gccgcgaccg ctggggcctg ctgcacctgc
     9661 tggaccgcga ggtcgaccgg atcggcgacc tggagagcag cctggcggtg gaggacctgc
     9721 tgatgagccg cctggaggaa ctgcgcgagg tggtcctggt gcccggggcg gacggcgaac
     9781 cggtccccgt cgtcgccacc gtcgacgaga gcccgctcga cgcggcgcgc tggcagcggg
     9841 cgaccagcga cctgccgacg atggcgccgg tgcgccagtt ccggttcgaa gacctgccgc
     9901 gcacgtccac ccgcaagatc cagaaaccgg aactggcccg gctgatccag ggcgtccggg
     9961 caaccgacca gggagtgggg gcatgaccta ggggaattgg atccactagt tctagaagct
    10021 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10981 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11041 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11101 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11161 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11221 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11281 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11341 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11401 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11461 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11521 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11581 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11641 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11701 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11761 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11821 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11881 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11941 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaa
//
