LOCUS       sequence-214249-        1208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of His-I3 V41A / W43 A in insect cells (Baculovirus 
            system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I,
            Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, 
            Meyer H
  TITLE     Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives
            PP1 Holoenzyme Formation.
  JOURNAL   Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 
            10.1016/j.molcel.2018.09.020.
  PUBMED    30344098
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 
            10.1016/j.molcel.2018.09.020."
COMMENT     Sequence Label: pFL His-I3 V41A / W43A
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..119
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             141..161
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    742..876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(779..798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     833..852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  986..1152
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tactgttttc gtacngtttt tgtataaaaa aacctataaa tattccggat tattcatacc
       61 gtcccaccat cgggcgcgga tctacaacca tgtcgtacta ccatcaccat caccatcacg
      121 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggga tccatggccg
      181 aggcaggggc tgggctgagc gagaccgtca ctgagacaac ggttaccgtg acaaccgagc
      241 ccgagaaccg gagccttacc atcaaacttc ggaaacggaa gccagagaaa aaggcagaag
      301 cgacaagtga cactgtggac aatgaacaca tgggccgccg ctcatccaaa tgctgctgta
      361 tttatgagaa acctcgggcc tttggcgaga gctccacgga aagtgatgag gaggaagaag
      421 agggctgtgg tcatacacac tgtgtacgtg gccaccgcaa aggacggcgt cgtgcaaccc
      481 taggaccgac ccccaccacc cctccccagc ctcctgaccc ttcccagccc cctccagggc
      541 caatgcagca ctaatgagtc gacgagctca ctagtcgcgg ccgctttcga atctagagcc
      601 tgcagtctcg acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
      661 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct ganacataaa
      721 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
      781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      841 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga
      901 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      961 actatcataa cccctaggag atccgaacca gataagtgaa atctagttcc aaactatttt
     1021 gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca
     1081 ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc caactanttt
     1141 tgtccgccca cagcggggnc atttttcttc ccggtnnggn ttttaatcaa acatcctggc
     1201 caactcca
//
