LOCUS       sequence-21426-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct for NF‐kappa-B activation. Contains two copies of
            immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream 
            of the a minimal promoter driving luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Pomerantz JL, Denny EM, Baltimore D
  TITLE     CARD11 mediates factor-specific activation of NF-kappaB by the T 
            cell receptor complex.
  JOURNAL   EMBO J. 2002 Oct 1. 21(19):5184-94.
  PUBMED    12356734
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Oct 1. 21(19):5184-94."
COMMENT     Sequence Label: IgK-IFN-luc
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    242..376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(266..285)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     320..339
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(385..404)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(386..404)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(411..428)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(411..427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(420..442)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccattt taccaacagt accggaatgc
       61 caagcttgcg aaaggttgca gttagaatgt cctttctcca tgggtatggc ctatttatat
      121 gagatggtcc tctctctatt cagcggatct cgacctgcag cccaagctcg ctctcggaaa
      181 gtcccctctg tagatctctc ggaaagtccc ctctgtgggc cgccaccgcg gtggggggat
      241 cgatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
      301 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
      361 ctgcaataaa caagttccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
      421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      601 ggtgtggtgg ttacgcgcag cgtgaccgct acactt
//
