LOCUS       sequence-214273-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MBP-GFP-DHFR; construct generated for pull-down and 
            localization experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Caldwell RM, Bermudez JG, Thai D, Aonbangkhen C, Schuster BS, 
            Courtney T, Deiters A, Hammer DA, Chenoweth DM, Good MC
  TITLE     Optochemical Control of Protein Localization and Activity within 
            Cell-like Compartments.
  JOURNAL   Biochemistry. 2018 May 8;57(18):2590-2596. doi: 
            10.1021/acs.biochem.8b00131. Epub 2018 Apr 19.
  PUBMED    29671583
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.8b00131"; journalName: "Biochemistry"; date: 
            "2018-05-8- 8"; volume: "57"; issue: "18"; pages: "2590-2596"
COMMENT     Sequence Label: pJB03
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..300
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     237..258
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             340..816
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
       61 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      121 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      181 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
      241 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      301 ctcgaggacg agctgtataa ggccggcctg gagtctagaa tcagtctgat tgcggcgtta
      361 gcggtagatc gcgttatcgg catggaaaac gccatgccgt ggaacctgcc tgccgatctc
      421 gcctggttta aacgcaacac cttaaataaa cccgtgatta tgggccgcca tacctgggaa
      481 tcaatcggtc gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgggtacg
      541 gacgatcgcg taacgtgggt gaagtcggtg gatgaagcca tcgcggcgtg tggtgacgta
      601 ccagaaatca tggtgattgg cggcggtcgc gtttatgaac agttcttgcc aaaagcgcaa
      661 aaactgtatc tgacgcatat cgacgcagaa gtggaaggcg acacccattt cccggattac
      721 gagccggatg actgggaatc ggtattcagc gaattccacg atgctgatgc gcagaactct
      781 cacagctatt gctttgagat tctggagcgg cggtaa
//
