LOCUS       sequence-214284-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA
  TITLE     Differential WNT activity in colorectal cancer confers limited 
            tumorigenic potential and is regulated by MAPK signaling.
  JOURNAL   Cancer Res. 2012 Feb 8.
  PUBMED    22318865
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2012 Feb 8."
COMMENT     Sequence Label: TOP-GFP.mC
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     regulatory      2030..2039
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2055..2064
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2061..2063,2064..2066,2067..2780)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2061..2063,2064..2066,2067..2777)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2106..2127)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2367..2386)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2714..2735
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2947..3064
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3110..3620
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3539..3557
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3635..4012
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             4019..4729
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4164..4182)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4432..4451
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4745..5333
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4798..4818)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5216..5227)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5420..5653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5725..5846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5762..5781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5816..5835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5886..6021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6041..6060)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6042..6060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6070..6087)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6070..6086)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6079..6101)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6228..6683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6315..6334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6525..6546
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6709..6813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6814..6882,6883..7674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7032..7051)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7845..8433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8334..8353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8587..8604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8721..8742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8757..8762,8763..8780,8781..8787)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8795..8811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8800..8822
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8819..8835
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8819..8835
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8854..8874
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8856..8874
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttat
     1801 cacgagacta gcctcgatag cccgggctcg agcagctgaa gcttgcatgc ctgcaggatc
     1861 aaagggggta agatcaaagg gggtaagatc aaaggtctag atcaaagggg gtaagatcaa
     1921 agggggtaag atcaaagggg gtaagatcaa aggctagagg gtatataatg gatccggtac
     1981 tcgagatctg cgatctaagt aagcttggca ttccggtact gttggtaaag ccaccatggg
     2041 atgatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2101 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2161 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2221 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2281 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2341 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2401 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2461 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2521 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2581 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2641 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2701 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2761 tggacgagct gtacaagtaa gcttagccat ggcttcccgc cggaggtgga ggagcaggat
     2821 gatggcacgc tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc
     2881 tgtgcttctg ctaggatcaa tgtgtaggcg gccgcctgca ggttaacaat caacctctgg
     2941 attaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     3001 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     3061 ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattccacg gggttggggt
     3121 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     3181 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     3241 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     3301 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     3361 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     3421 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     3481 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     3541 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     3601 tcaccgacct ctctccccag ggggatccgc caccatgcca gagccagcga agtctgctcc
     3661 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag acggcaagaa
     3721 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     3781 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     3841 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     3901 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     3961 gcacgccgtg tccgagggta ctaaggccgt caccaagtac accagcgcta aggatccgat
     4021 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     4081 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     4141 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     4201 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     4261 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4321 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     4381 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4441 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4501 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     4561 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     4621 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     4681 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcgt
     4741 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4801 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4861 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4921 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4981 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5041 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5101 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     5161 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5221 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5281 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     5341 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5401 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5461 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5521 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5581 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5641 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5701 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5821 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     5881 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5941 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6001 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6061 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6181 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6241 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6301 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6361 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6421 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6481 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6541 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6601 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6661 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6721 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6781 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6841 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6901 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6961 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7021 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7081 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7141 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7201 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7261 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7321 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7381 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7441 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7501 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7561 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7621 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7681 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7741 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7801 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7861 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7921 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7981 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8041 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8101 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8161 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8221 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8281 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8341 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8401 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8461 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8521 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8581 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8641 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8701 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8761 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8821 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     8881 aagctggagc tgcaagctta a
//
