LOCUS       sequence-214291-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mouse BK channel alpha subunit expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Gruslova A, Semenov I, Wang B
  TITLE     An extracellular domain of the accessory beta1 subunit is required 
            for modulating BK channel voltage sensor and gate.
  JOURNAL   J Gen Physiol. 2012 Jan;139(1):57-67. doi: 10.1085/jgp.201110698. 
            Epub 2011 Dec 12.
  PUBMED    22155735
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1085/jgp.201110698"; journalName: "J Gen Physiol"; date: 
            "2012-01"; volume: "139"; issue: "1"; pages: "57-67"
COMMENT     Sequence Label: BK alpha subunit
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3918..3936)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3919..3936)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3956..3973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3962..4186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4232..4660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4319..4338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4529..4550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4669..4689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4674..5004
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4855..4990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4917..4936
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5071..5865
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5125..5144)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5735..5754
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6039..6160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6076..6095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6130..6149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6209..6225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6209..6225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6222..6244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6233..6249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6257..6263,6264..6281,6282..6287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6302..6323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6440..6457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6611..7199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6691..6710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7370..8161,8162..8230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7993..8012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8231..8335)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8410..8429)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8601..8980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8981..9184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9135..9155
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9229..9248
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9229..9247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcttggtac ctcgagcccc tgcgcctgcc gcccattgct agctatggca aacggtggcg
       61 gcggaggcgg cggcagcagc ggcggcggcg gcggcggcgg cggaggcagc ggtcttagaa
      121 tgagcagcaa tatccacgcg aaccatctca gcctagacgc gtcctcctcc tcctcttcct
      181 cctcctcttc ttcttcttcc tcctcctctt cctcctcgtc ctcggtccac gagcccaaga
      241 tggatgcgct catcataccg gtgaccatgg aggtgccgtg cgacagccgg ggccaacgca
      301 tgtggtgggc tttcttggcc tcctccatgg tgactttctt cgggggcctc ttcatcatct
      361 tgctctggcg gacgctcaag tacctgtgga ccgtttgctg ccactgcggg ggcaagacga
      421 aggaggccca gaagataaac aatggctcca gccaggcaga tggtactctc aagccagtgg
      481 acgaaaaaga ggaggtggtg gcagccgagg tcggctggat gacatctgtg aaggactggg
      541 caggggtgat gatatccgcc cagacactga ctggcagagt cctggttgtg ttagtctttg
      601 ctctcagcat tggtgccctc gtaatatact tcatagactc gtcaaaccca atagaatcct
      661 gccagaattt ctacaaagat ttcacattac agatcgacat ggctttcaac gtgttcttcc
      721 tcctctactt tggcttgcgg tttattgcag ccaacgataa gctgtggttc tggctggaag
      781 tgaattcagt agtagatttc ttcacagtcc ctcctgtgtt tgtgtctgtg tacttaaaca
      841 gaagttggct tggcttgaga tttttaagag ctctcagact gatacagttt tcagagattt
      901 tgcagtttct gaatatcctt aaaacaagta actccatcaa gctggtgaat ctgctctcca
      961 tatttatcag cacgtggctg actgcagctg gattcatcca cttggtggag aattcagggg
     1021 acccatggga aaatttccaa aacaaccagg cacttacgta ctgggaatgt gtctacttac
     1081 tcatggtcac aatgtctaca gtgggttatg gggacgttta tgcaaaaacc acacttggac
     1141 gcctcttcat ggtcttcttc atcctcgggg gactggccat gtttgcctcg tacgtccctg
     1201 aaatcataga gttaatagga aaccgcaaga aatacggggg ctcctatagc gcggttagtg
     1261 gaagaaagca cattgtagtc tgtggacaca ttactctgga gagtgtctct aacttcctga
     1321 aggactttct gcacaaggac cgggatgatg tcaacgtgga gattgtcttt cttcacaaca
     1381 tctcccctaa ccttgagctt gaagctctgt tcaaacggca tttcactcag gtggagtttt
     1441 atcagggctc tgtcctcaat ccacatgatc ttgccagagt caagatagag tcagcagatg
     1501 catgcctgat ccttgccaat aagtattgcg ctgacccgga tgcagaagat gcctccaaca
     1561 tcatgagagt gatctccatc aaaaactacc acccaaagat caggatcatc actcagatgc
     1621 tgcagtatca caacaaggcc catctgctca acatccccag ctggaactgg aaagagggtg
     1681 atgacgcaat atgccttgca gagctcaagt tgggtttcat agcccagagc tgtctggctc
     1741 aaggcctctc cacaatgctt gccaatctct tctctatgag gtcattcata aagattgagg
     1801 aagacacatg gcagaaatac tacttggaag gagtctccaa tgaaatgtac acagaatatc
     1861 tctccagtgc cttcgtgggt ctgtccttcc ctactgtttg tgagctgtgt tttgtgaagc
     1921 ttaagctcct gatgatagcc attgagtaca agtctgccaa cagagagagc cgaagccgaa
     1981 agcgaatatt aattaaccct gggaaccacc ttaagatcca agaaggtact ttaggatttt
     2041 tcatcgcaag tgatgccaaa gaagttaaaa gggcattttt ttactgcaag gcctgtcatg
     2101 atgacgtcac agatcccaaa agaattaaaa aatgtggctg caggcggctt gaagatgagc
     2161 agccgccaac cctgtcacca aaaaaaaaac aacgtaatgg gggcatgagg aactcgccca
     2221 acacctcccc gaagctgatg aggcatgacc ccttgttaat tcctggcaat gatcagattg
     2281 acaacatgga ctccaatgtg aaaaagtacg actccactgg aatgtttcac tggtgtgcac
     2341 ccaaggagat tgagaaagtc atcttgactc gaagtgaagc tgccatgact gtcctgagtg
     2401 gccatgtcgt agtctgcatc tttggggatg tcagctcagc cctgattggc ctccggaacc
     2461 tggtgatgcc acttcgtgct agcaactttc actatcatga gctcaaacac attgtgtttg
     2521 tgggctccat tgagtacctc aagagggagt gggaaacact gcacaacttc ccgaaagtgt
     2581 ccatattgcc tggtacacca ttaagtcggg ctgatttaag ggctgtcaac atcaacctct
     2641 gtgacatgtg cgttatcctg tcagccaatc agaataatat tgatgatact tcgcttcagg
     2701 acaaggaatg catcttggcg tcactcaaca tcaaatctat gcagtttgat gacagcatcg
     2761 gggtcttgca ggctaattcc caaggattca cacctcctgg aatggacaga tcatcacccg
     2821 acaacagccc agtgcacggg atgttacgcc agccgtccat cacaactggg gtcaacattc
     2881 ccatcatcac ggaactcgct aagccgggca agttgccttt ggtatcagtc aatcaggaaa
     2941 aaaacagtgg gacgcacatt ctaatgataa cggagttggt gaatgatacc aatgttcagt
     3001 ttttggacca agacgatgac gatgaccctg acacagagct gtacctcaca cagccctttg
     3061 cttgtgggac agcatttgcc gtcagcgtcc tggactcact catgagcgcg acatacttca
     3121 atgacaatat cctcacccta atacggaccc tggtgacagg aggagccaca ccagagctcg
     3181 aggctctaat agctgaggag aatgcacttc gaggaggcta cagcactccg cagacattgg
     3241 ccaacaggga ccgttgccga gtggcccagt tagccctgtt agatggtccc tttgcagact
     3301 taggggatgg tggttgttat ggtgatctgt tctgcaaagc tctgaaaaca tataatatgc
     3361 tttgttttgg aatttaccgg ctgagagatg cccacctcag cacccccagc cagtgtacaa
     3421 aaaggtacgt catcaccaat cctccctacg agtttgagct cgtaccaaca gacctgatct
     3481 tctgcctgat gcagtttgac cacaacgctg gccaatcccg ggccagtctg tctcattcct
     3541 cccactcctc acagtcgtcc agtaagaaga gctcctccgt ccactccatc ccgtccacag
     3601 caaatcggcc gaaccggccc aagtccaggg agtcccgcga caaacagaac agaaaagaaa
     3661 tggtttacag atgagccgga taatgcctat cccagaaaca ttcaaatcaa gcccatgagt
     3721 acccacatgg ctaaccagat caaccaatat aaatccacaa gcagcctgat ccctccaatc
     3781 agagaagttg aagatgaatg ttgactccca ggagaccaga actatttttt taaagcctga
     3841 caaacttcat aaatggtgac gtgacttttc ttcctcttac atgctgaatc actggtgaaa
     3901 attctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     3961 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4021 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4081 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4141 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4201 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4681 agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat
     4741 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4801 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4861 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4921 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     4981 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5041 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     5101 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5161 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5221 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5281 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5341 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5401 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5461 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5521 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5581 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5641 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5701 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5761 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5821 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5881 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5941 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6001 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6181 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6241 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6301 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6361 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6421 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6781 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     6841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7021 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8341 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc
     8401 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8461 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8521 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     8581 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     8641 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     8701 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     8761 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac
     8821 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     8881 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     8941 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     9001 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9061 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     9121 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9181 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     9241 ctatagggag acccaagct
//
