LOCUS       sequence-214328-        8457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor
            away Vipp1 (sll0617) of Synecocystis PCC6803..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH50
FEATURES             Location/Qualifiers
     source          1..8457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1537..1863
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLET"
     CDS             complement(2316..3107)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(4782..4800)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4783..4800)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4818..4834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4818..4834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4831..4853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4842..4858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4866..4872,4873..4890,4891..4896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4911..4932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5049..5066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5220..5808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5300..5319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5979..6770,6771..6839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6602..6621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6840..6944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7012..7030
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7068..7090)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7190..7209
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7276..7731)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7413..7434)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7625..7644
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7857..7879
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7871..7888
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7872..7888
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7895..7914
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7895..7913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgggattat ttgaccgttt aggccgcgtc gtccgggcta acctcaacga cctcgtcagt
       61 aaagctgaag atccagaaaa agttctggaa caggccgtga tcgatatgca ggaagacctg
      121 gtgcaactcc gccaggccgt tgcccgcacg atcgccgaag aaaaacgaac agagcaacgt
      181 cttaaccaag acacccagga agccaagaaa tgggaagacc gggcaaaatt agccctcacc
      241 aatggggaag aaaatttggc tagggaagcc ctggcccgca aaaaaagtct gacagatacg
      301 gcggcggcgt accaaaccca gctagcccaa cagaggacca tgtcggagaa tctccgtcgc
      361 aacctcgcgg ctctagaagc aaaaatttcc gaagctaaaa ccaagaaaaa tatgttgcag
      421 gccagggcca aagcggccaa ggctaatgct gaactgcagc aaaccctcgg gggcttaggt
      481 actagcagtg ctactagtgc ttttgaacgg atggagaaca aggtactgga tatggaagcc
      541 acttcccaag cggcagggga gttagccggc tttggcatcg agaaccagtt tgcccagttg
      601 gaagccagca gtggggtaga agacgagttg gccgccctga aagcttccat ggccggtgga
      661 gcattaccgg gaacctctgc cgctacgccc caactcgaag cggctcctgt tgattcctca
      721 gtaccagcta ataatgccag tcaagatgat gcggtgattg accaggaatt ggacgaccta
      781 cgtcgtcggt taaataatct gagtggtggt ggaggtagca aaggcgagga gctctttact
      841 ggcgttgtcc ccattctcgt cgaactagac ggggatgtta atggccataa gttctccgtg
      901 agtggcgaag gggaaggtga tgccacgtat ggcaaactga cgctgaaatt tatctgtacc
      961 accgggaaat tgccggttcc ttggccaaca ctcgttacca cctttggcta tggcgtccaa
     1021 tgctttgccc gttaccccga tcacatgaag cagcatgact tcttcaaaag cgccatgcca
     1081 gagggttatg tccaagaacg cacgatcttc ttcaaggacg atggcaacta caagacccga
     1141 gctgaagtga agtttgaggg agatacgttg gtgaatcgca tcgaactgaa agggatcgac
     1201 tttaaagagg atggcaatat tctggggcat aaactggagt acaactacaa ttcgcataac
     1261 gtgtacatta tggcggataa gcagaagaat ggcatcaagg tcaacttcaa gattcggcac
     1321 aatatcgaag atggttcagt gcaactagcg gaccactatc agcagaacac accgattggc
     1381 gatggtcctg tcctgttgcc ggataaccac tatctgagca ctcaatcgaa actgtctaag
     1441 gatcccaacg agaaacgcga tcacatggtg ttgctcgaat ttgtgactgc tgcaggcatt
     1501 acccatggaa tggacgaact gtacaaagga agtggtggcg ttcaagtgga gacaatttct
     1561 cctggcgacg gtcgtacctt tcccaaacgg ggacaaacgt gcgtggtcca ttataccggt
     1621 atgctggaag atggcaaaaa atttgatagc agccgtgatc gtaataaacc ctttaaattt
     1681 atgctaggga aacaagaggt aattcggggg tgggaagagg gagtggccca aatgtctgtc
     1741 gggcagcgag ctaaattgac tatctctcct gattacgcat atggcgcgac tgggcatccc
     1801 ggcatcattc cccctcatgc caccctagtc tttgatgttg aattgttaaa attagaaaca
     1861 tagttggttt aactcagcca gtaaaaatcc cccattaacc taggttttgg gggaaattga
     1921 tcagattggc ggatttggcc ggggttcagg ttgtaaaccg ttttgtgaaa aaatttttaa
     1981 aataaaaaag gggacctcta gggtccccaa ttaattagta atataatcta ttaaaggtca
     2041 ttcaaaaggt catccaccgg atcagcttag taaagccctc gctagatttt aatgcggatg
     2101 ttgcgattac ttcgccaact attgcgataa caagaaaaag ccagcctttc atgatatatc
     2161 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
     2221 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc
     2281 gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
     2341 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     2401 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     2461 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     2521 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     2581 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     2641 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     2701 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
     2761 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     2821 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
     2881 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     2941 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     3001 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     3061 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacttt
     3121 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     3181 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa
     3241 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     3301 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag
     3361 gcttatgtcc actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     3421 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagga ttgaattaca
     3481 aatgctagtg ggtcagactc cagcaactat gattaggggt tcgagtttcc ctacaggaag
     3541 caatcaaagc ctcgatcacc cacagccaga actgggcaat attaccagct agacttggtt
     3601 acaccgggca aaagaccctg gtgggcccat tcccgtaaaa cattgcggca aaggccaaag
     3661 tcacggtagt aggaacgggg acgacctgta acctgacagc ggttacggcg acggttgggg
     3721 gcgctattgc ggggtaaatc ctgcaatttt tggtgaacag ctaatttgtc ttctagggtt
     3781 tcagcttgac ggaattcttc tttgagcgct tcccgtttag cagcatactt tgctaccaag
     3841 cggctacgac gcttatcccg ctcgatcatg gatttttttg ccatcggggt ttaactttat
     3901 ttctttagtc taaaaattga gcactcccca tcatatcaca ctgcttgggg acacaaaggt
     3961 ttatgaaagg taatggaact agacgaacac ggagggattc gaacccccga ccctcaggac
     4021 cggaacctga tgctctatcc actgagctac gtgtccagga cagacttggg ctgacccttt
     4081 catcttgcga aaagaattca caccagctat cagcgtaaca tttcctgcac cgattcggaa
     4141 gctaactggg caccattttc cagtaaccaa gcttcctgac tgggagctaa ccccggattg
     4201 tcaccaaaac tgagattttc cacctttgcg ccactgaaat tgacgttttg gagagtggct
     4261 ccccgcaaat ccgcttcccg taggtctgtg ccgattaaag ttgctagggc taaactactt
     4321 ttacgaaaat caacttggct gagattagcc ccttctaggt aagcgccact caggtctgca
     4381 ccccgaaaat tggaccggcg gagatcggta ttactcaaat cgctgtcggt cagaatagct
     4441 ccccggaaat tgctgtccat tagttggcca ttactaaggt caatgctgtt gaggttagca
     4501 cccacaaatt tgcctcccgc caaatcccgt tgcgggtcca aaccgactaa cttccccagg
     4561 gtaaggcaat ccaactgggg ggagttgatg attaattgca ggcgatcgcc aaggttcccg
     4621 gtgtctgctt ccgttacggt gcgattattt tcgacattgt atagaggttg ccagataatt
     4681 tgaggatcgg catcgactga tttgctcagg agccattgac cccaacaaag ggccggagtt
     4741 aggtaatttt ccgctataaa cttacatgca agcttgagta ttctatagtg tcacctaaat
     4801 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4861 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4921 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4981 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5041 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5641 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6961 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7021 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7081 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7141 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7201 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7261 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     7321 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     7381 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     7441 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     7501 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     7561 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     7621 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     7681 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     7741 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     7801 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     7861 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     7921 cgagctcggt acccgggtaa cgaaaggatt cggcgggtag ggcaaaatct tgtcagcaga
     7981 ctaggaatag caattaagcc caagcccaac tactttaact gttcaagttg tttttccctc
     8041 atttcactgg gctttttaat atttggcttt ggagccatcc ccaataattt ttgattaatt
     8101 tgttccctgg tgtttttgct ataactagcc cagggatcgg gcaaaaacat atcaccgatg
     8161 ctgataaaag ccagaccagc aatcaccgct ggaatggcaa gggagcgcaa aattttaaat
     8221 agacttgcca taacgctatg cctccacaat caaatctcga ttggactacc tgtgtttcca
     8281 ttctagggta gggcaccatg gcgaaagcca ctaaaaccaa ctccaaggct gtctagtgtc
     8341 tgcgggcttg gtttacactg gatcaacata gggccaactc cttagtcccc cttatgcgga
     8401 cttctgtaca gacccccatt cgttaacccc cagcgatttt gagaggataa gtaagac
//
