LOCUS       sequence-21436-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Gallegos LL, Kunkel MT, Newton AC
  TITLE     Targeting protein kinase C activity reporter to discrete 
            intracellular regions reveals spatiotemporal differences in 
            agonist-dependent signaling.
  JOURNAL   J Biol Chem. 2006 Oct 13. 281(41):30947-56.
  PUBMED    16901905
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 Oct 13. 281(41):30947-56."
COMMENT     Sequence Label: NucCKAR
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..257)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMEELY"
     primer_bind     complement(48..69)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     397..416
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgggctggt acagctcttc catgccgtga gtgatcccgg cggcggtcac gaactccagc
       61 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gcttggactg gtagctcagg
      121 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      181 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
      241 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
      301 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      361 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      421 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      481 tgcttcatgt ggtcggggta gcgggcgaag cacatcacgc cgtagccgaa ggtggtcacg
      541 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
      601 ccgtaagtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      661 ccgtccagct c
//
