LOCUS       sequence-21439-e         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Gallegos LL, Kunkel MT, Newton AC
  TITLE     Targeting protein kinase C activity reporter to discrete 
            intracellular regions reveals spatiotemporal differences in 
            agonist-dependent signaling.
  JOURNAL   J Biol Chem. 2006 Oct 13. 281(41):30947-56.
  PUBMED    16901905
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 Oct 13. 281(41):30947-56."
COMMENT     Sequence Label: MitoCKAR
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(254..275)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcagagctct ctggctacta gagaaccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttatggtggg acggaacagc gccatcgctg caggagtgtg
      121 cggtgccctc ttcatagggt actgcatcta ctttgaccgc aaaaggagga gtgaccccaa
      181 cggtggaggt ggtggaggag gtggaggtgg tgtgagcaag ggcgaggagc tgttcaccgg
      241 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaggt tcagcgtgtc
      301 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      361 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
      421 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      481 aggctacgtc caggagcgta ccatcttctt caaggacgac ggcnactaca agacccgcgc
      541 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgact
//
