LOCUS       sequence-214407-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PE_4: Prhl_lac_bD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Chen Y, Ho JML, Shis DL, Gupta C, Long J, Wagner DS, Ott W, Josic K,
            Bennett MR
  TITLE     Tuning the dynamic range of bacterial promoters regulated by 
            ligand-inducible transcription factors.
  JOURNAL   Nat Commun. 2018 Jan 4;9(1):64. doi: 10.1038/s41467-017-02473-5.
  PUBMED    29302024
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-4"; volume: "9(1):64. doi"; pages: " 
            10.1038/s41467-017-02473-5"
COMMENT     Sequence Label: PE_4: Prhl_lac_bD
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..714
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
       61 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
      121 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg
      181 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
      241 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
      301 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
      361 aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag
      421 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
      481 attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat
      541 cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat
      601 ctgagctacc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg
      661 ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgatga
//
