LOCUS       sequence-21441-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Gallegos LL, Kunkel MT, Newton AC
  TITLE     Targeting protein kinase C activity reporter to discrete 
            intracellular regions reveals spatiotemporal differences in 
            agonist-dependent signaling.
  JOURNAL   J Biol Chem. 2006 Oct 13. 281(41):30947-56.
  PUBMED    16901905
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 Oct 13. 281(41):30947-56."
COMMENT     Sequence Label: YFP-DBD
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(337..358)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(598..617)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttcgccacc atgggcccgg cctctgatga cccacggagc
      121 aaacacaagt ttaagatcca cacctactcc agccctacct tctgtgacca ctgtggatcc
      181 ctgctgtggg ggctcatcca ccaggggatg aaatgcgaca cctgtatgat gaatgtccac
      241 aagcgctgcg tgatgaacgt ccccagcctc tgtggcggag gtctcgaggc actggtgagc
      301 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      361 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      421 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      481 accttcggct acggcctgaa gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      541 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      601 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      661 atcgagctg
//
