LOCUS       sequence-21443-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Kunkel MT, Ni Q, Tsien RY, Zhang J, Newton AC
  TITLE     Spatio-temporal dynamics of protein kinase B/Akt signaling revealed 
            by a genetically encoded fluorescent reporter.
  JOURNAL   J Biol Chem. 2005 Feb 18. 280(7):5581-7.
  PUBMED    15583002
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Feb 18. 280(7):5581-7."
COMMENT     Sequence Label: BKAR-T/A
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(77..79,80..592)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(119..140)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(380..399)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggtgaaaag ttaggaggat ccggaggtag gaagcgtgac agacttggag ctctaggaat
       61 cggtggatct ggtggagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      121 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      181 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      241 gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc
      301 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      361 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      421 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      481 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      541 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      601 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      661 gcccgacaac cactacctg
//
