LOCUS       sequence-214458-        9341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Zhang Q, Zheng YW, Coughlin SR, Shu X
  TITLE     A rapid fluorogenic GPCR-beta-arrestin interaction assay.
  JOURNAL   Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 
            2018 Feb 23.
  PUBMED    29411438
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3385"; 
            journalName: "Protein Sci"; date: "2018-04"; volume: "27"; issue: 
            "4"; pages: "874-879"
COMMENT     Sequence Label: PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCh...
FEATURES             Location/Qualifiers
     source          1..9341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2360..2413
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2420..2429
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2516..2536
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2570..2839
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             4133..4186
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4193..4903
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4338..4356)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4606..4625
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4935..4952)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4941..5165
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5211..5639
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5298..5317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5508..5529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5648..5668)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5653..5982
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5833..5968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6049..6843
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6103..6122)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6713..6732
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7017..7138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7054..7073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7108..7127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7187..7203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7187..7203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7200..7222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7211..7227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7235..7241,7242..7259,7260..7265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7280..7301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7418..7435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7589..8174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7669..7688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8345..9136,9137..9205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8968..8987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9206..9310)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggacta caaggacgat gatgacgcca tgatcgatat
      961 ggggccccgg cgcttgctga tcgtcgccct cggcctcagc ctgtgcggtc ccttgctgtc
     1021 ttcccgcgtc cctatgagcc agccagaatc agagaggaca gatgctacgg tgaacccccg
     1081 ctcattcttt ctaaggaatc ccagtgaaaa tacatttgaa ctggtccccc tgggggatga
     1141 ggaggaggag gagaaaaatg aaagcgtcct gctggagggt agggcagtct acttaaatat
     1201 aagcctccct cctcacacgc cgcctcctcc cttcatctcc gaggacgcct ccggatatct
     1261 gaccagcccc tggctgacgc tcttcatgcc ctccgtgtac acgattgtgt tcattgtcag
     1321 ccttcctctg aacgtcctgg ccatcgcagt gttcgtcttg aggatgaagg tcaagaagcc
     1381 ggccgtggtg tacatgctgc acctggccat ggccgacgtg ctcttcgtgt cggtgctccc
     1441 cttcaagatc agctactact tctccggcac tgattggcag ttcgggtctg gaatgtgccg
     1501 cttcgccacc gcagcgtttt acgggaacat gtacgcctcc atcatgctca tgacggtcat
     1561 aagcattgac cggttcctgg cggtggtgta tccgatccag tccctgtcct ggcgcactct
     1621 gggccgagcc aacttcactt gcgtggtcat ttgggtgatg gccatcatgg gggtggtgcc
     1681 ccttctcctc aaggagcaga ccacccgagt tccgggactc aacatcacca cctgccacga
     1741 cgtcctcagt gagaacctga tgcaaggctt ttactcgtac tacttctcgg ccttctccgc
     1801 catcttcttt cttgtgccgt tgatcgtttc cacggtctgc tacacgtcca tcatccggtg
     1861 cctgagctcc tccgcggttg ccaaccggag caagaagtcg cgggctttgt tcctgtctgc
     1921 cgcggtgttc tgcatcttca tcgtctgctt tgggcccacc aacgtcctcc tgattgtgca
     1981 ctaccttttc ctctccgaca gtcctggtac agaggcagcc tactttgctt acctcctctg
     2041 cgtctgtgtg agcagcgtga gctgctgcat cgatccgttg atttactact acgcctcctc
     2101 cgagtgccag aggcacctct acagcatctt gtgctgcaaa gaaagctctg atcccaacag
     2161 ttgcaacagc accggccagc tgatgccgag taaaatggat acctgctcta gtcacctgaa
     2221 taacagcata tacaaaaagc tattagcttt aattaactct ggtggcggga gtggtggcgg
     2281 gggtaccatg gaaaagcgag accatatggt tttgcttgag tatgttacag cggctggcat
     2341 taccgatgca tcaggatccg agggcagagg aagtctgcta acatgcggtg acgtcgagga
     2401 gaatcctggc ccagaattcg ccaccatgga cctgcctgac gaccactacc tgtccaccca
     2461 gaccatcctg tccaaggacc tgaactccgg actcagatct ggcagcggtg gaggcgaaaa
     2521 tctctacttc cagagtagcg catccggcgg aagcggaagc ctcgagatgg agatgtggca
     2581 cgagggactc gaagaggcca gcaggctgta ctttggcgag aggaacgtca agggcatgtt
     2641 cgaagtgctg gagcccctcc atgcgatgat ggaaaggggc ccacagaccc tgaaggagac
     2701 cagcttcaac caggcttacg gcagggacct gatggaggca caggaatggt gcaggaagta
     2761 catgaagagc ggcaacgtga aagacctgac ccaggcgtgg gacctctact accacgtgtt
     2821 caggaggatc agcaagcagg gcggaggagg cagcgggcgc gccatgggcg acaaagggac
     2881 acgagtgttc aagaaggcaa gccccaatgg aaagctcact gtctacctgg gaaagcggga
     2941 ctttgtggac cacattgacc tcgtggaccc cgtggatggt gtggtcctgg tggatcctga
     3001 gtatctcaaa gaaaggcgag tctacgtgac actgacctgc gccttccggt atggccggga
     3061 agacctggat gtcttgggtc tgacttttcg gaaagacctg tttgtggcca atgtgcagtc
     3121 cttcccaccg gcccccgagg acaagaagcc actgacacgg ctacaggagc gactcatcaa
     3181 gaagctgggc gagcatgcct gccccttcac ctttgagatc ccgccaaacc ttccatgctc
     3241 agtcactctg cagcctgggc ctgaggacac aggaaaggcc tgtggtgtgg attatgaagt
     3301 caaagccttc tgtgctgaga acctggagga gaagatccac aaaaggaact ctgtgcggct
     3361 agtcatccgg aaggttcagt atgcccctga gagacctggc cctcagccca cggctgagac
     3421 taccagacag ttccttatgt cagacaagcc cctacacctt gaggcatcac tggataagga
     3481 gatctattac cacggagaac ccatcagcgt taatgtccac gtcaccaaca acaccaacaa
     3541 gactgtgaag aagatcaaga tctcggtgcg ccagtatgca gacatctgtc tcttcaacac
     3601 agctcagtac aagtgcccag tggccatgga ggaggctgat gacaatgtgg cacccagctc
     3661 aacattctgc aaggtctata cgctgactcc cttcctggcc aacaaccgag agaagcgggg
     3721 actcgccctc gacgggaagc tcaagcatga ggacacgaat ctggcttcca gcacgctgtt
     3781 gcgggaaggt gccaaccgtg aaatcctggg catcatcgtt tcctacaaag tcaaggtgaa
     3841 actggtggtg tcccggggcg gcctcttggg agaccttgca tccagtgatg tggccgtgga
     3901 gctgcctttt accctaatgc accccaagcc taaggaggag cccccacatc gggaagttcc
     3961 agagagcgag actccagtag acaccaatct catagagctt gacaccaatg atgacgacat
     4021 tgtatttgag gactttgctc gccagcggct gaaaggcatg aaggatgaca aggacgaaga
     4081 ggatgacggc accggctctc cgcacctcaa caacagaggc ggccgctcta gagagggcag
     4141 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagaat tgatggtgag
     4201 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     4261 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4321 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     4381 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     4441 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4501 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     4561 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     4621 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     4681 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4741 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4801 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     4861 gggccgccac tccaccggcg gcatggacga gctgtacaag taatctagag ggcccgttta
     4921 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4981 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5041 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5101 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     5161 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     5221 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5281 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5341 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5401 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5461 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5521 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5581 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5641 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5701 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5761 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5821 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5881 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5941 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     6001 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     6061 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6121 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6181 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6241 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6301 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6361 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6421 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6481 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6541 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6601 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6661 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6721 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6781 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6841 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6901 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6961 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7141 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7201 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7261 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7321 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7381 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7441 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7501 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7561 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7621 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7681 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7741 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7801 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7861 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7921 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7981 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8101 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
