LOCUS       sequence-214463-        9329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Zhang Q, Zheng YW, Coughlin SR, Shu X
  TITLE     A rapid fluorogenic GPCR-beta-arrestin interaction assay.
  JOURNAL   Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 
            2018 Feb 23.
  PUBMED    29411438
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3385"; 
            journalName: "Protein Sci"; date: "2018-04"; volume: "27"; issue: 
            "4"; pages: "874-879"
COMMENT     Sequence Label: PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCh...
FEATURES             Location/Qualifiers
     source          1..9329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2348..2401
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2408..2417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2504..2524
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2558..2827
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             4121..4174
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4181..4891
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4326..4344)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4594..4613
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4923..4940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4929..5153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5199..5627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5286..5305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5496..5517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5636..5656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5641..5970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5821..5956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5883..5902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6037..6831
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6091..6110)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6701..6720
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7005..7126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7042..7061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7096..7115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7175..7191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7175..7191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7188..7210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7199..7215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7223..7229,7230..7247,7248..7253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7268..7289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7406..7423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7577..8162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7657..7676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8333..9124,9125..9193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8956..8975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9194..9298)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgaagac gatcatcgcc ctgagctaca tcttctgcct
      961 ggtgttcgcc gactacaagg acgatgatga cgccatgggg caacccggga acggcagcgc
     1021 cttcttgctg gcacccaata gaagccatgc gccggaccac gacgtcacgc agcaaaggga
     1081 cgaggtgtgg gtggtgggca tgggcatcgt catgtctctc atcgtcctgg ccatcgtgtt
     1141 tggcaatgtg ctggtcatca cagccattgc caagttcgag cgtctgcaga cggtcaccaa
     1201 ctacttcatc acttcactgg cctgtgctga tctggtcatg ggcctggcag tggtgccctt
     1261 tggggccgcc catattctta tgaaaatgtg gacttttggc aacttctggt gcgagttttg
     1321 gacttccatt gatgtgctgt gcgtcacggc cagcattgag accctgtgcg tgatcgcagt
     1381 ggatcgctac tttgccatta cttcaccttt caagtaccag agcctgctga ccaagaataa
     1441 ggcccgggtg atcattctga tggtgtggat tgtgtcaggc cttacctcct tcttgcccat
     1501 tcagatgcac tggtatcggg ccacccacca ggaagccatc aactgctatg ccaatgagac
     1561 ctgctgtgac ttcttcacga accaagccta tgccattgcc tcttccatcg tgtccttcta
     1621 cgttcccctg gtgatcatgg tcttcgtcta ctccagggtc tttcaggagg ccaaaaggca
     1681 gctccagaag attgacaaat ctgagggccg cttccatgtc cagaacctta gccaggtgga
     1741 gcaggatggg cggacggggc atggactccg cagatcttcc aagttctgct tgaaggagca
     1801 caaagccctc aagacgttag gcatcatcat gggcactttc accctctgct ggctgccctt
     1861 cttcatcgtt aacattgtgc atgtgatcca ggataacctc atccgtaagg aagtttacat
     1921 cctcctaaat tggataggct atgtcaattc tggtttcaat ccccttatct actgccggag
     1981 cccagatttc aggattgcct tccaggagct tctgtgcctg cgcaggtctt ctttgaaggc
     2041 ctatgggaat ggctactcca gcaacggcaa cacaggggag cagagtggat atcacgtgga
     2101 acaggagaaa gaaaataaac tgctgtgtga agacctccca ggcacggaag actttgtggg
     2161 ccatcaaggt actgtgccta gcgataacat cgattcacaa gggaggaatt gtagtacaaa
     2221 tgactcactg ctggcattaa ttaactctgg tggcgggagt ggtggcgggg gtaccatgga
     2281 aaagcgagac catatggttt tgcttgagta tgttacagcg gctggcatta ccgatgcatc
     2341 aggatccgag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     2401 agaattcgcc accatggacc tgcctgacga ccactacctg tccacccaga ccatcctgtc
     2461 caaggacctg aactccggac tcagatctgg cagcggtgga ggcgaaaatc tctacttcca
     2521 gagtagcgca tccggcggaa gcggaagcct cgagatggag atgtggcacg agggactcga
     2581 agaggccagc aggctgtact ttggcgagag gaacgtcaag ggcatgttcg aagtgctgga
     2641 gcccctccat gcgatgatgg aaaggggccc acagaccctg aaggagacca gcttcaacca
     2701 ggcttacggc agggacctga tggaggcaca ggaatggtgc aggaagtaca tgaagagcgg
     2761 caacgtgaaa gacctgaccc aggcgtggga cctctactac cacgtgttca ggaggatcag
     2821 caagcagggc ggaggaggca gcgggcgcgc catgggcgac aaagggacac gagtgttcaa
     2881 gaaggcaagc cccaatggaa agctcactgt ctacctggga aagcgggact ttgtggacca
     2941 cattgacctc gtggaccccg tggatggtgt ggtcctggtg gatcctgagt atctcaaaga
     3001 aaggcgagtc tacgtgacac tgacctgcgc cttccggtat ggccgggaag acctggatgt
     3061 cttgggtctg acttttcgga aagacctgtt tgtggccaat gtgcagtcct tcccaccggc
     3121 ccccgaggac aagaagccac tgacacggct acaggagcga ctcatcaaga agctgggcga
     3181 gcatgcctgc cccttcacct ttgagatccc gccaaacctt ccatgctcag tcactctgca
     3241 gcctgggcct gaggacacag gaaaggcctg tggtgtggat tatgaagtca aagccttctg
     3301 tgctgagaac ctggaggaga agatccacaa aaggaactct gtgcggctag tcatccggaa
     3361 ggttcagtat gcccctgaga gacctggccc tcagcccacg gctgagacta ccagacagtt
     3421 ccttatgtca gacaagcccc tacaccttga ggcatcactg gataaggaga tctattacca
     3481 cggagaaccc atcagcgtta atgtccacgt caccaacaac accaacaaga ctgtgaagaa
     3541 gatcaagatc tcggtgcgcc agtatgcaga catctgtctc ttcaacacag ctcagtacaa
     3601 gtgcccagtg gccatggagg aggctgatga caatgtggca cccagctcaa cattctgcaa
     3661 ggtctatacg ctgactccct tcctggccaa caaccgagag aagcggggac tcgccctcga
     3721 cgggaagctc aagcatgagg acacgaatct ggcttccagc acgctgttgc gggaaggtgc
     3781 caaccgtgaa atcctgggca tcatcgtttc ctacaaagtc aaggtgaaac tggtggtgtc
     3841 ccggggcggc ctcttgggag accttgcatc cagtgatgtg gccgtggagc tgccttttac
     3901 cctaatgcac cccaagccta aggaggagcc cccacatcgg gaagttccag agagcgagac
     3961 tccagtagac accaatctca tagagcttga caccaatgat gacgacattg tatttgagga
     4021 ctttgctcgc cagcggctga aaggcatgaa ggatgacaag gacgaagagg atgacggcac
     4081 cggctctccg cacctcaaca acagaggcgg ccgctctaga gagggcagag gaagtctgct
     4141 aacatgcggt gacgtcgagg agaatcctgg cccagaattg atggtgagca agggcgagga
     4201 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     4261 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     4321 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     4381 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     4441 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     4501 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     4561 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     4621 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     4681 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     4741 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     4801 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     4861 caccggcggc atggacgagc tgtacaagta atctagaggg cccgtttaaa cccgctgatc
     4921 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4981 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5041 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5101 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5161 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5221 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5281 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5341 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5401 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5461 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5521 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5581 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5641 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5701 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5761 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5881 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5941 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6001 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6061 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6121 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6181 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6241 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6301 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6361 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6421 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6481 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6541 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6601 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6661 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6721 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6781 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6841 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6901 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6961 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7021 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7081 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7141 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7201 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7261 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8101 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9301 catttccccg aaaagtgcca cctgacgtc
//
